Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24250 and PS417_24255 are separated by 70 nucleotides PS417_24255 and PS417_24260 overlap by 4 nucleotides PS417_24260 and PS417_24265 are separated by 347 nucleotides
PS417_24250: PS417_24250 - hypothetical protein, at 5,260,811 to 5,261,167
_24250
PS417_24255: PS417_24255 - elongation factor GreAB, at 5,261,238 to 5,261,735
_24255
PS417_24260: PS417_24260 - glutamate dehydrogenase, at 5,261,732 to 5,263,069
_24260
PS417_24265: PS417_24265 - ABC transporter ATP-binding protein, at 5,263,417 to 5,265,081
_24265
Position (kb) 5261
5262
5263
5264 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5260.737 kb on + strand at 5260.737 kb on + strand at 5260.738 kb on - strand at 5260.769 kb on + strand at 5260.769 kb on + strand at 5260.769 kb on + strand at 5260.769 kb on + strand at 5260.770 kb on - strand at 5260.805 kb on + strand at 5261.194 kb on - strand at 5261.223 kb on + strand at 5261.223 kb on + strand at 5261.224 kb on - strand at 5261.382 kb on + strand, within PS417_24255 at 5261.382 kb on + strand, within PS417_24255 at 5261.383 kb on - strand, within PS417_24255 at 5261.383 kb on - strand, within PS417_24255 at 5261.454 kb on + strand, within PS417_24255 at 5261.516 kb on - strand, within PS417_24255 at 5261.516 kb on - strand, within PS417_24255 at 5261.733 kb on + strand at 5261.802 kb on + strand at 5261.803 kb on - strand at 5261.803 kb on - strand at 5261.803 kb on - strand at 5262.055 kb on + strand, within PS417_24260 at 5262.055 kb on + strand, within PS417_24260 at 5262.339 kb on + strand, within PS417_24260 at 5262.474 kb on + strand, within PS417_24260 at 5262.475 kb on - strand, within PS417_24260 at 5262.529 kb on - strand, within PS417_24260 at 5262.529 kb on - strand, within PS417_24260 at 5262.529 kb on - strand, within PS417_24260 at 5262.804 kb on + strand, within PS417_24260 at 5262.821 kb on - strand, within PS417_24260 at 5262.971 kb on + strand at 5262.971 kb on + strand at 5262.972 kb on - strand at 5262.972 kb on - strand at 5262.972 kb on - strand at 5262.981 kb on - strand at 5262.992 kb on + strand at 5263.187 kb on + strand at 5263.188 kb on - strand at 5263.192 kb on - strand at 5263.245 kb on - strand at 5263.245 kb on - strand at 5263.245 kb on - strand at 5263.248 kb on + strand at 5263.249 kb on - strand at 5263.249 kb on - strand at 5263.282 kb on + strand at 5263.282 kb on + strand at 5263.506 kb on + strand at 5263.798 kb on + strand, within PS417_24265 at 5263.802 kb on + strand, within PS417_24265 at 5263.802 kb on + strand, within PS417_24265 at 5263.802 kb on + strand, within PS417_24265 at 5263.803 kb on - strand, within PS417_24265 at 5263.823 kb on + strand, within PS417_24265 at 5263.823 kb on + strand, within PS417_24265 at 5263.824 kb on - strand, within PS417_24265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,260,737 + +0.5 5,260,737 + -1.0 5,260,738 - +0.8 5,260,769 + -1.0 5,260,769 + -0.0 5,260,769 + +0.5 5,260,769 + +1.3 5,260,770 - -1.5 5,260,805 + +0.3 5,261,194 - -0.5 5,261,223 + +0.3 5,261,223 + +2.0 5,261,224 - +0.5 5,261,382 + PS417_24255 0.29 +0.5 5,261,382 + PS417_24255 0.29 +2.0 5,261,383 - PS417_24255 0.29 +1.0 5,261,383 - PS417_24255 0.29 +1.2 5,261,454 + PS417_24255 0.43 -0.9 5,261,516 - PS417_24255 0.56 +1.1 5,261,516 - PS417_24255 0.56 -1.3 5,261,733 + +0.0 5,261,802 + -3.5 5,261,803 - -3.1 5,261,803 - -0.5 5,261,803 - -1.0 5,262,055 + PS417_24260 0.24 -1.4 5,262,055 + PS417_24260 0.24 -0.7 5,262,339 + PS417_24260 0.45 +0.5 5,262,474 + PS417_24260 0.55 +0.2 5,262,475 - PS417_24260 0.56 -0.3 5,262,529 - PS417_24260 0.60 -2.4 5,262,529 - PS417_24260 0.60 -0.2 5,262,529 - PS417_24260 0.60 +0.5 5,262,804 + PS417_24260 0.80 -3.3 5,262,821 - PS417_24260 0.81 -3.0 5,262,971 + +0.0 5,262,971 + -2.4 5,262,972 - +1.3 5,262,972 - -2.3 5,262,972 - -1.9 5,262,981 - -0.2 5,262,992 + -1.5 5,263,187 + -1.3 5,263,188 - -1.2 5,263,192 - +0.4 5,263,245 - +0.5 5,263,245 - -0.5 5,263,245 - -4.0 5,263,248 + -1.7 5,263,249 - +1.8 5,263,249 - +0.2 5,263,282 + +0.4 5,263,282 + -1.4 5,263,506 + -0.8 5,263,798 + PS417_24265 0.23 -0.3 5,263,802 + PS417_24265 0.23 -1.5 5,263,802 + PS417_24265 0.23 +0.5 5,263,802 + PS417_24265 0.23 -1.2 5,263,803 - PS417_24265 0.23 -2.2 5,263,823 + PS417_24265 0.24 -2.0 5,263,823 + PS417_24265 0.24 -0.1 5,263,824 - PS417_24265 0.24 +0.2
Or see this region's nucleotide sequence