Strain Fitness in Pseudomonas simiae WCS417 around PS417_24205

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_24195 and PS417_24200 are separated by 144 nucleotidesPS417_24200 and PS417_24205 are separated by 167 nucleotidesPS417_24205 and PS417_24210 are separated by 124 nucleotides PS417_24195: PS417_24195 - GNAT family acetyltransferase, at 5,248,201 to 5,248,749 _24195 PS417_24200: PS417_24200 - GCN5 family acetyltransferase, at 5,248,894 to 5,249,331 _24200 PS417_24205: PS417_24205 - hypothetical protein, at 5,249,499 to 5,250,524 _24205 PS417_24210: PS417_24210 - dolichyl-phosphate-mannose-protein mannosyltransferase, at 5,250,649 to 5,252,082 _24210 Position (kb) 5249 5250 5251Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5248.588 kb on + strand, within PS417_24195at 5248.667 kb on - strand, within PS417_24195at 5248.667 kb on - strand, within PS417_24195at 5248.747 kb on + strandat 5248.779 kb on + strandat 5248.780 kb on - strandat 5248.780 kb on - strandat 5248.802 kb on - strandat 5248.815 kb on - strandat 5248.815 kb on - strandat 5248.838 kb on + strandat 5248.975 kb on + strandat 5248.984 kb on - strand, within PS417_24200at 5248.987 kb on - strand, within PS417_24200at 5248.990 kb on - strand, within PS417_24200at 5248.990 kb on - strand, within PS417_24200at 5248.990 kb on - strand, within PS417_24200at 5249.064 kb on - strand, within PS417_24200at 5249.118 kb on + strand, within PS417_24200at 5249.119 kb on - strand, within PS417_24200at 5249.187 kb on - strand, within PS417_24200at 5249.224 kb on + strand, within PS417_24200at 5249.307 kb on + strandat 5249.307 kb on + strandat 5249.377 kb on - strandat 5249.409 kb on - strandat 5249.409 kb on - strandat 5249.409 kb on - strandat 5249.603 kb on + strand, within PS417_24205at 5249.643 kb on + strand, within PS417_24205at 5249.643 kb on + strand, within PS417_24205at 5249.647 kb on - strand, within PS417_24205at 5249.649 kb on + strand, within PS417_24205at 5249.649 kb on + strand, within PS417_24205at 5249.650 kb on - strand, within PS417_24205at 5249.650 kb on - strand, within PS417_24205at 5249.758 kb on + strand, within PS417_24205at 5249.758 kb on + strand, within PS417_24205at 5249.758 kb on + strand, within PS417_24205at 5249.758 kb on + strand, within PS417_24205at 5249.759 kb on - strand, within PS417_24205at 5249.850 kb on + strand, within PS417_24205at 5249.850 kb on + strand, within PS417_24205at 5249.850 kb on + strand, within PS417_24205at 5249.850 kb on + strand, within PS417_24205at 5249.851 kb on - strand, within PS417_24205at 5249.851 kb on - strand, within PS417_24205at 5249.920 kb on - strand, within PS417_24205at 5249.920 kb on - strand, within PS417_24205at 5249.984 kb on + strand, within PS417_24205at 5250.042 kb on + strand, within PS417_24205at 5250.043 kb on - strand, within PS417_24205at 5250.045 kb on - strand, within PS417_24205at 5250.114 kb on + strand, within PS417_24205at 5250.115 kb on - strand, within PS417_24205at 5250.273 kb on + strand, within PS417_24205at 5250.363 kb on + strand, within PS417_24205at 5250.363 kb on + strand, within PS417_24205at 5250.363 kb on + strand, within PS417_24205at 5250.364 kb on - strand, within PS417_24205at 5250.453 kb on - strandat 5250.453 kb on - strandat 5250.540 kb on + strandat 5250.541 kb on - strandat 5250.938 kb on - strand, within PS417_24210at 5251.015 kb on + strand, within PS417_24210at 5251.015 kb on + strand, within PS417_24210at 5251.290 kb on + strand, within PS417_24210at 5251.290 kb on + strand, within PS417_24210at 5251.295 kb on + strand, within PS417_24210at 5251.296 kb on - strand, within PS417_24210at 5251.426 kb on + strand, within PS417_24210at 5251.427 kb on - strand, within PS417_24210at 5251.439 kb on - strand, within PS417_24210at 5251.439 kb on - strand, within PS417_24210at 5251.439 kb on - strand, within PS417_24210at 5251.441 kb on + strandat 5251.441 kb on + strand, within PS417_24210at 5251.441 kb on + strand, within PS417_24210at 5251.442 kb on - strand, within PS417_24210at 5251.442 kb on - strand, within PS417_24210at 5251.444 kb on + strand, within PS417_24210at 5251.444 kb on + strand, within PS417_24210at 5251.444 kb on + strand, within PS417_24210at 5251.445 kb on - strand, within PS417_24210at 5251.445 kb on - strand, within PS417_24210at 5251.445 kb on - strand, within PS417_24210at 5251.492 kb on + strand, within PS417_24210

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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5,248,588 + PS417_24195 0.70 +2.3
5,248,667 - PS417_24195 0.85 +0.9
5,248,667 - PS417_24195 0.85 -0.1
5,248,747 + +0.5
5,248,779 + -0.9
5,248,780 - +1.4
5,248,780 - +0.3
5,248,802 - +0.8
5,248,815 - +0.7
5,248,815 - -2.7
5,248,838 + -2.7
5,248,975 + +0.4
5,248,984 - PS417_24200 0.21 -2.6
5,248,987 - PS417_24200 0.21 -1.6
5,248,990 - PS417_24200 0.22 -0.1
5,248,990 - PS417_24200 0.22 -1.2
5,248,990 - PS417_24200 0.22 -0.8
5,249,064 - PS417_24200 0.39 -1.1
5,249,118 + PS417_24200 0.51 +1.4
5,249,119 - PS417_24200 0.51 -0.3
5,249,187 - PS417_24200 0.67 -2.7
5,249,224 + PS417_24200 0.75 -2.4
5,249,307 + +0.4
5,249,307 + +0.8
5,249,377 - -1.0
5,249,409 - +0.0
5,249,409 - +1.7
5,249,409 - +0.7
5,249,603 + PS417_24205 0.10 +0.3
5,249,643 + PS417_24205 0.14 +0.7
5,249,643 + PS417_24205 0.14 +0.5
5,249,647 - PS417_24205 0.14 +0.7
5,249,649 + PS417_24205 0.15 -2.5
5,249,649 + PS417_24205 0.15 +0.4
5,249,650 - PS417_24205 0.15 -0.1
5,249,650 - PS417_24205 0.15 -0.4
5,249,758 + PS417_24205 0.25 -0.1
5,249,758 + PS417_24205 0.25 +0.3
5,249,758 + PS417_24205 0.25 -2.4
5,249,758 + PS417_24205 0.25 -0.8
5,249,759 - PS417_24205 0.25 -2.8
5,249,850 + PS417_24205 0.34 -0.2
5,249,850 + PS417_24205 0.34 +1.9
5,249,850 + PS417_24205 0.34 -0.2
5,249,850 + PS417_24205 0.34 +0.8
5,249,851 - PS417_24205 0.34 +1.4
5,249,851 - PS417_24205 0.34 +0.9
5,249,920 - PS417_24205 0.41 -1.0
5,249,920 - PS417_24205 0.41 -1.4
5,249,984 + PS417_24205 0.47 -2.9
5,250,042 + PS417_24205 0.53 -2.2
5,250,043 - PS417_24205 0.53 -0.9
5,250,045 - PS417_24205 0.53 -0.1
5,250,114 + PS417_24205 0.60 +1.1
5,250,115 - PS417_24205 0.60 +0.5
5,250,273 + PS417_24205 0.75 -1.5
5,250,363 + PS417_24205 0.84 +0.3
5,250,363 + PS417_24205 0.84 -1.4
5,250,363 + PS417_24205 0.84 +0.8
5,250,364 - PS417_24205 0.84 -2.6
5,250,453 - +1.7
5,250,453 - +1.2
5,250,540 + -0.5
5,250,541 - +0.9
5,250,938 - PS417_24210 0.20 -1.4
5,251,015 + PS417_24210 0.26 -1.7
5,251,015 + PS417_24210 0.26 -3.6
5,251,290 + PS417_24210 0.45 -2.3
5,251,290 + PS417_24210 0.45 -1.1
5,251,295 + PS417_24210 0.45 -0.9
5,251,296 - PS417_24210 0.45 +1.0
5,251,426 + PS417_24210 0.54 +0.8
5,251,427 - PS417_24210 0.54 -0.8
5,251,439 - PS417_24210 0.55 -0.6
5,251,439 - PS417_24210 0.55 +1.0
5,251,439 - PS417_24210 0.55 -0.7
5,251,441 + +0.8
5,251,441 + PS417_24210 0.55 +0.5
5,251,441 + PS417_24210 0.55 -0.3
5,251,442 - PS417_24210 0.55 -0.5
5,251,442 - PS417_24210 0.55 +2.4
5,251,444 + PS417_24210 0.55 -0.7
5,251,444 + PS417_24210 0.55 +0.3
5,251,444 + PS417_24210 0.55 +0.3
5,251,445 - PS417_24210 0.56 -1.2
5,251,445 - PS417_24210 0.56 -1.0
5,251,445 - PS417_24210 0.56 +0.4
5,251,492 + PS417_24210 0.59 +0.2

Or see this region's nucleotide sequence