Strain Fitness in Pseudomonas simiae WCS417 around PS417_00240

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00230 and PS417_00235 are separated by 86 nucleotidesPS417_00235 and PS417_00240 overlap by 4 nucleotidesPS417_00240 and PS417_00245 are separated by 102 nucleotides PS417_00230: PS417_00230 - aminopeptidase, at 44,748 to 45,791 _00230 PS417_00235: PS417_00235 - HAD family hydrolase, at 45,878 to 46,537 _00235 PS417_00240: PS417_00240 - anhydrase, at 46,534 to 47,076 _00240 PS417_00245: PS417_00245 - oligopeptidase A, at 47,179 to 49,230 _00245 Position (kb) 46 47 48Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 45.586 kb on - strand, within PS417_00230at 45.642 kb on + strand, within PS417_00230at 45.642 kb on + strand, within PS417_00230at 45.643 kb on - strand, within PS417_00230at 45.643 kb on - strand, within PS417_00230at 45.875 kb on - strandat 45.892 kb on + strandat 46.137 kb on + strand, within PS417_00235at 46.137 kb on + strand, within PS417_00235at 46.218 kb on + strand, within PS417_00235at 46.218 kb on + strand, within PS417_00235at 46.218 kb on + strand, within PS417_00235at 46.219 kb on - strand, within PS417_00235at 46.288 kb on - strand, within PS417_00235at 46.360 kb on - strand, within PS417_00235at 46.468 kb on + strand, within PS417_00235at 46.580 kb on + strandat 46.595 kb on + strand, within PS417_00240at 46.654 kb on + strand, within PS417_00240at 46.654 kb on + strand, within PS417_00240at 46.654 kb on + strand, within PS417_00240at 46.654 kb on + strand, within PS417_00240at 46.654 kb on + strand, within PS417_00240at 46.655 kb on - strand, within PS417_00240at 46.655 kb on - strand, within PS417_00240at 46.658 kb on + strand, within PS417_00240at 46.658 kb on + strand, within PS417_00240at 46.658 kb on + strand, within PS417_00240at 46.658 kb on + strand, within PS417_00240at 46.658 kb on + strand, within PS417_00240at 46.658 kb on + strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 46.659 kb on - strand, within PS417_00240at 47.079 kb on + strandat 47.102 kb on - strandat 47.102 kb on - strandat 47.123 kb on + strandat 47.124 kb on - strandat 47.128 kb on - strandat 47.128 kb on - strandat 47.150 kb on + strandat 47.154 kb on + strandat 47.154 kb on + strandat 47.155 kb on - strandat 47.155 kb on - strandat 47.210 kb on - strandat 47.210 kb on - strandat 47.444 kb on - strand, within PS417_00245at 47.473 kb on + strand, within PS417_00245at 47.474 kb on - strand, within PS417_00245at 47.843 kb on - strand, within PS417_00245at 47.843 kb on - strand, within PS417_00245at 47.843 kb on - strand, within PS417_00245at 47.843 kb on - strand, within PS417_00245at 47.843 kb on - strand, within PS417_00245at 47.846 kb on - strand, within PS417_00245at 47.860 kb on + strand, within PS417_00245at 47.861 kb on - strand, within PS417_00245at 47.902 kb on + strand, within PS417_00245at 47.902 kb on + strand, within PS417_00245at 47.908 kb on - strand, within PS417_00245at 48.011 kb on - strand, within PS417_00245at 48.011 kb on - strand, within PS417_00245

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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45,586 - PS417_00230 0.80 +1.2
45,642 + PS417_00230 0.86 -1.0
45,642 + PS417_00230 0.86 -1.8
45,643 - PS417_00230 0.86 +2.2
45,643 - PS417_00230 0.86 +1.1
45,875 - -2.6
45,892 + -0.4
46,137 + PS417_00235 0.39 -3.6
46,137 + PS417_00235 0.39 +1.2
46,218 + PS417_00235 0.52 +0.2
46,218 + PS417_00235 0.52 -0.7
46,218 + PS417_00235 0.52 +0.9
46,219 - PS417_00235 0.52 +0.4
46,288 - PS417_00235 0.62 +2.3
46,360 - PS417_00235 0.73 +0.1
46,468 + PS417_00235 0.89 -2.4
46,580 + -0.3
46,595 + PS417_00240 0.11 -1.9
46,654 + PS417_00240 0.22 -1.9
46,654 + PS417_00240 0.22 -0.2
46,654 + PS417_00240 0.22 +0.0
46,654 + PS417_00240 0.22 +1.9
46,654 + PS417_00240 0.22 +0.2
46,655 - PS417_00240 0.22 +0.3
46,655 - PS417_00240 0.22 +0.4
46,658 + PS417_00240 0.23 -0.7
46,658 + PS417_00240 0.23 -0.1
46,658 + PS417_00240 0.23 +1.5
46,658 + PS417_00240 0.23 +0.2
46,658 + PS417_00240 0.23 -0.4
46,658 + PS417_00240 0.23 -1.4
46,659 - PS417_00240 0.23 +1.1
46,659 - PS417_00240 0.23 +1.3
46,659 - PS417_00240 0.23 -0.5
46,659 - PS417_00240 0.23 +0.9
46,659 - PS417_00240 0.23 +0.1
46,659 - PS417_00240 0.23 -3.7
46,659 - PS417_00240 0.23 +0.1
46,659 - PS417_00240 0.23 -2.1
46,659 - PS417_00240 0.23 +2.1
46,659 - PS417_00240 0.23 -0.4
46,659 - PS417_00240 0.23 -0.2
47,079 + -0.2
47,102 - -2.6
47,102 - -1.9
47,123 + -0.3
47,124 - -0.4
47,128 - -0.4
47,128 - -0.0
47,150 + +0.6
47,154 + -0.5
47,154 + +2.5
47,155 - -1.1
47,155 - -0.3
47,210 - -0.3
47,210 - -0.3
47,444 - PS417_00245 0.13 -1.9
47,473 + PS417_00245 0.14 -2.5
47,474 - PS417_00245 0.14 +1.2
47,843 - PS417_00245 0.32 +2.5
47,843 - PS417_00245 0.32 +1.5
47,843 - PS417_00245 0.32 -2.2
47,843 - PS417_00245 0.32 +1.0
47,843 - PS417_00245 0.32 +0.0
47,846 - PS417_00245 0.33 +0.8
47,860 + PS417_00245 0.33 +1.7
47,861 - PS417_00245 0.33 +0.2
47,902 + PS417_00245 0.35 +1.6
47,902 + PS417_00245 0.35 +1.8
47,908 - PS417_00245 0.36 +2.8
48,011 - PS417_00245 0.41 -0.3
48,011 - PS417_00245 0.41 +1.2

Or see this region's nucleotide sequence