Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23835 and PS417_23840 are separated by 192 nucleotides PS417_23840 and PS417_23845 are separated by 251 nucleotides PS417_23845 and PS417_23850 overlap by 1 nucleotides
PS417_23835: PS417_23835 - hypothetical protein, at 5,159,192 to 5,159,536
_23835
PS417_23840: PS417_23840 - competence protein TfoX, at 5,159,729 to 5,160,001
_23840
PS417_23845: PS417_23845 - hemin ABC transporter ATP-binding protein, at 5,160,253 to 5,161,020
_23845
PS417_23850: PS417_23850 - ABC transporter permease, at 5,161,020 to 5,162,057
_23850
Position (kb) 5160
5161
5162 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 5159.338 kb on + strand, within PS417_23835 at 5159.366 kb on + strand, within PS417_23835 at 5159.456 kb on + strand, within PS417_23835 at 5159.456 kb on + strand, within PS417_23835 at 5159.456 kb on + strand, within PS417_23835 at 5159.457 kb on - strand, within PS417_23835 at 5159.610 kb on + strand at 5159.662 kb on - strand at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.891 kb on + strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5159.892 kb on - strand, within PS417_23840 at 5160.025 kb on - strand at 5160.169 kb on + strand at 5160.169 kb on + strand at 5160.170 kb on - strand at 5160.170 kb on - strand at 5160.226 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.230 kb on + strand at 5160.232 kb on + strand at 5160.319 kb on + strand at 5160.319 kb on + strand at 5160.320 kb on - strand at 5160.320 kb on - strand at 5160.320 kb on - strand at 5160.360 kb on + strand, within PS417_23845 at 5160.630 kb on - strand, within PS417_23845 at 5160.897 kb on + strand, within PS417_23845 at 5160.898 kb on - strand, within PS417_23845 at 5160.898 kb on - strand, within PS417_23845 at 5160.898 kb on - strand, within PS417_23845 at 5160.973 kb on + strand at 5161.038 kb on + strand at 5161.045 kb on + strand at 5161.307 kb on + strand, within PS417_23850 at 5161.307 kb on + strand, within PS417_23850 at 5161.442 kb on - strand, within PS417_23850 at 5161.442 kb on - strand, within PS417_23850 at 5161.442 kb on - strand, within PS417_23850 at 5161.510 kb on + strand, within PS417_23850 at 5161.514 kb on + strand, within PS417_23850 at 5161.626 kb on - strand, within PS417_23850 at 5161.666 kb on + strand, within PS417_23850 at 5161.666 kb on + strand, within PS417_23850 at 5161.667 kb on - strand, within PS417_23850 at 5161.821 kb on + strand, within PS417_23850 at 5161.822 kb on - strand, within PS417_23850 at 5161.940 kb on + strand, within PS417_23850
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,159,338 + PS417_23835 0.42 -1.0 5,159,366 + PS417_23835 0.50 -1.3 5,159,456 + PS417_23835 0.77 +1.3 5,159,456 + PS417_23835 0.77 +2.5 5,159,456 + PS417_23835 0.77 +0.6 5,159,457 - PS417_23835 0.77 +1.1 5,159,610 + +0.7 5,159,662 - -2.4 5,159,891 + PS417_23840 0.59 -0.2 5,159,891 + PS417_23840 0.59 +0.1 5,159,891 + PS417_23840 0.59 -1.0 5,159,891 + PS417_23840 0.59 +1.3 5,159,891 + PS417_23840 0.59 -1.4 5,159,892 - PS417_23840 0.60 +0.0 5,159,892 - PS417_23840 0.60 -0.3 5,159,892 - PS417_23840 0.60 -0.8 5,159,892 - PS417_23840 0.60 +2.8 5,160,025 - -1.8 5,160,169 + +1.1 5,160,169 + +1.1 5,160,170 - +0.9 5,160,170 - -5.3 5,160,226 + +0.3 5,160,230 + -0.9 5,160,230 + +0.3 5,160,230 + +2.3 5,160,230 + +1.9 5,160,230 + +0.2 5,160,230 + +0.2 5,160,232 + +1.6 5,160,319 + +1.8 5,160,319 + -0.2 5,160,320 - -0.8 5,160,320 - +2.0 5,160,320 - +0.5 5,160,360 + PS417_23845 0.14 -0.5 5,160,630 - PS417_23845 0.49 -3.6 5,160,897 + PS417_23845 0.84 -1.5 5,160,898 - PS417_23845 0.84 -2.0 5,160,898 - PS417_23845 0.84 -0.3 5,160,898 - PS417_23845 0.84 -1.2 5,160,973 + -1.0 5,161,038 + -1.8 5,161,045 + -0.9 5,161,307 + PS417_23850 0.28 -2.8 5,161,307 + PS417_23850 0.28 +0.8 5,161,442 - PS417_23850 0.41 +0.1 5,161,442 - PS417_23850 0.41 +0.0 5,161,442 - PS417_23850 0.41 +0.0 5,161,510 + PS417_23850 0.47 +0.5 5,161,514 + PS417_23850 0.48 -2.2 5,161,626 - PS417_23850 0.58 +0.3 5,161,666 + PS417_23850 0.62 +1.4 5,161,666 + PS417_23850 0.62 +0.3 5,161,667 - PS417_23850 0.62 +0.7 5,161,821 + PS417_23850 0.77 +1.1 5,161,822 - PS417_23850 0.77 +0.2 5,161,940 + PS417_23850 0.89 -2.1
Or see this region's nucleotide sequence