Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23705 and PS417_23710 overlap by 4 nucleotides PS417_23710 and PS417_23715 are separated by 385 nucleotides PS417_23715 and PS417_23720 are separated by 48 nucleotides
PS417_23705: PS417_23705 - Tryptophan 2,3-dioxygenase (EC 1.13.11.11) (from data) , at 5,125,994 to 5,126,851
_23705
PS417_23710: PS417_23710 - Kynurenine formamidase, bacterial (EC 3.5.1.9) (from data) , at 5,126,848 to 5,127,498
_23710
PS417_23715: PS417_23715 - cupin, at 5,127,884 to 5,128,228
_23715
PS417_23720: PS417_23720 - 3-hydroxyisobutyrate dehydrogenase, at 5,128,277 to 5,129,164
_23720
Position (kb)
5126
5127
5128 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5125.899 kb on - strand at 5125.995 kb on + strand at 5126.058 kb on + strand at 5126.058 kb on + strand at 5126.185 kb on - strand, within PS417_23705 at 5126.214 kb on + strand, within PS417_23705 at 5126.422 kb on + strand, within PS417_23705 at 5126.505 kb on + strand, within PS417_23705 at 5126.505 kb on + strand, within PS417_23705 at 5126.757 kb on + strand, within PS417_23705 at 5127.158 kb on + strand, within PS417_23710 at 5127.189 kb on + strand, within PS417_23710 at 5127.189 kb on + strand, within PS417_23710 at 5127.269 kb on + strand, within PS417_23710 at 5127.269 kb on + strand, within PS417_23710 at 5127.269 kb on + strand, within PS417_23710 at 5127.270 kb on - strand, within PS417_23710 at 5127.314 kb on + strand, within PS417_23710 at 5127.315 kb on - strand, within PS417_23710 at 5127.532 kb on + strand at 5127.533 kb on - strand at 5127.594 kb on + strand at 5127.622 kb on + strand at 5127.622 kb on + strand at 5127.626 kb on + strand at 5127.627 kb on - strand at 5127.627 kb on - strand at 5127.627 kb on - strand at 5127.627 kb on - strand at 5127.629 kb on - strand at 5127.629 kb on - strand at 5127.696 kb on + strand at 5127.710 kb on + strand at 5127.710 kb on + strand at 5127.710 kb on + strand at 5127.710 kb on + strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.903 kb on + strand at 5128.052 kb on + strand, within PS417_23715 at 5128.052 kb on + strand, within PS417_23715 at 5128.052 kb on + strand, within PS417_23715 at 5128.052 kb on + strand at 5128.053 kb on - strand, within PS417_23715 at 5128.061 kb on + strand, within PS417_23715 at 5128.062 kb on - strand, within PS417_23715 at 5128.062 kb on - strand, within PS417_23715 at 5128.098 kb on - strand, within PS417_23715 at 5128.207 kb on + strand at 5128.208 kb on - strand at 5128.279 kb on - strand at 5128.300 kb on + strand at 5128.301 kb on - strand at 5128.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,125,899 - +1.1 5,125,995 + -0.8 5,126,058 + -0.4 5,126,058 + +1.4 5,126,185 - PS417_23705 0.22 -1.3 5,126,214 + PS417_23705 0.26 -2.2 5,126,422 + PS417_23705 0.50 -0.4 5,126,505 + PS417_23705 0.60 -1.0 5,126,505 + PS417_23705 0.60 +1.5 5,126,757 + PS417_23705 0.89 -1.8 5,127,158 + PS417_23710 0.48 +0.3 5,127,189 + PS417_23710 0.52 -2.1 5,127,189 + PS417_23710 0.52 -0.8 5,127,269 + PS417_23710 0.65 -2.0 5,127,269 + PS417_23710 0.65 -0.4 5,127,269 + PS417_23710 0.65 +0.9 5,127,270 - PS417_23710 0.65 -0.9 5,127,314 + PS417_23710 0.72 -0.2 5,127,315 - PS417_23710 0.72 -0.4 5,127,532 + -0.6 5,127,533 - -0.2 5,127,594 + -0.1 5,127,622 + +0.2 5,127,622 + +0.4 5,127,626 + +1.2 5,127,627 - +3.7 5,127,627 - -1.5 5,127,627 - +1.3 5,127,627 - +1.2 5,127,629 - -0.3 5,127,629 - +0.3 5,127,696 + +0.4 5,127,710 + -0.8 5,127,710 + -1.4 5,127,710 + -0.3 5,127,710 + -0.7 5,127,711 - +0.8 5,127,711 - +0.7 5,127,711 - -0.9 5,127,711 - -0.0 5,127,711 - -1.7 5,127,903 + +2.3 5,128,052 + PS417_23715 0.49 -2.7 5,128,052 + PS417_23715 0.49 -1.9 5,128,052 + PS417_23715 0.49 -2.4 5,128,052 + +1.8 5,128,053 - PS417_23715 0.49 -0.3 5,128,061 + PS417_23715 0.51 +0.2 5,128,062 - PS417_23715 0.52 -0.7 5,128,062 - PS417_23715 0.52 -1.9 5,128,098 - PS417_23715 0.62 +0.2 5,128,207 + -2.5 5,128,208 - +1.9 5,128,279 - -0.1 5,128,300 + -0.1 5,128,301 - +0.5 5,128,304 - -0.5
Or see this region's nucleotide sequence