Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23660 and PS417_23665 are separated by 41 nucleotides PS417_23665 and PS417_23670 are separated by 12 nucleotides
PS417_23660: PS417_23660 - Catechol 1,2-dioxygenase (EC 1.13.11.1) (from data) , at 5,116,586 to 5,117,515
_23660
PS417_23665: PS417_23665 - Muconolactone isomerase (EC 5.3.3.4) (from data) , at 5,117,557 to 5,117,847
_23665
PS417_23670: PS417_23670 - Muconate cycloisomerase (EC 5.5.1.1) (from data) , at 5,117,860 to 5,118,987
_23670
Position (kb)
5117
5118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5116.587 kb on + strand at 5117.029 kb on - strand, within PS417_23660 at 5117.029 kb on - strand, within PS417_23660 at 5117.029 kb on - strand, within PS417_23660 at 5117.032 kb on + strand, within PS417_23660 at 5117.187 kb on + strand, within PS417_23660 at 5117.187 kb on + strand, within PS417_23660 at 5117.187 kb on + strand, within PS417_23660 at 5117.188 kb on - strand, within PS417_23660 at 5117.188 kb on - strand, within PS417_23660 at 5117.188 kb on - strand, within PS417_23660 at 5117.188 kb on - strand, within PS417_23660 at 5117.268 kb on + strand, within PS417_23660 at 5117.269 kb on - strand, within PS417_23660 at 5117.322 kb on - strand, within PS417_23660 at 5117.334 kb on + strand at 5117.335 kb on - strand, within PS417_23660 at 5117.335 kb on - strand, within PS417_23660 at 5117.335 kb on - strand, within PS417_23660 at 5117.335 kb on - strand, within PS417_23660 at 5117.535 kb on - strand at 5117.538 kb on + strand at 5117.538 kb on + strand at 5117.539 kb on - strand at 5117.539 kb on - strand at 5117.539 kb on - strand at 5117.539 kb on - strand at 5117.541 kb on + strand at 5117.541 kb on + strand at 5117.541 kb on + strand at 5117.621 kb on + strand, within PS417_23665 at 5117.621 kb on + strand, within PS417_23665 at 5117.621 kb on + strand, within PS417_23665 at 5117.691 kb on - strand, within PS417_23665 at 5117.691 kb on - strand, within PS417_23665 at 5117.691 kb on - strand, within PS417_23665 at 5117.861 kb on + strand at 5117.862 kb on - strand at 5118.309 kb on + strand, within PS417_23670 at 5118.310 kb on - strand, within PS417_23670 at 5118.310 kb on - strand, within PS417_23670 at 5118.696 kb on + strand, within PS417_23670 at 5118.697 kb on - strand, within PS417_23670 at 5118.789 kb on + strand, within PS417_23670 at 5118.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,116,587 + +1.6 5,117,029 - PS417_23660 0.48 +0.4 5,117,029 - PS417_23660 0.48 -3.4 5,117,029 - PS417_23660 0.48 +0.5 5,117,032 + PS417_23660 0.48 -0.3 5,117,187 + PS417_23660 0.65 -1.2 5,117,187 + PS417_23660 0.65 -0.5 5,117,187 + PS417_23660 0.65 -1.0 5,117,188 - PS417_23660 0.65 -2.6 5,117,188 - PS417_23660 0.65 -2.0 5,117,188 - PS417_23660 0.65 +0.0 5,117,188 - PS417_23660 0.65 -3.1 5,117,268 + PS417_23660 0.73 +0.5 5,117,269 - PS417_23660 0.73 +2.0 5,117,322 - PS417_23660 0.79 -1.8 5,117,334 + -0.2 5,117,335 - PS417_23660 0.81 -0.0 5,117,335 - PS417_23660 0.81 -2.9 5,117,335 - PS417_23660 0.81 -0.1 5,117,335 - PS417_23660 0.81 +0.2 5,117,535 - +1.6 5,117,538 + -0.3 5,117,538 + +0.4 5,117,539 - +0.3 5,117,539 - +1.1 5,117,539 - -0.8 5,117,539 - -0.3 5,117,541 + -1.1 5,117,541 + +1.5 5,117,541 + -1.9 5,117,621 + PS417_23665 0.22 +0.0 5,117,621 + PS417_23665 0.22 +0.0 5,117,621 + PS417_23665 0.22 +0.8 5,117,691 - PS417_23665 0.46 +2.0 5,117,691 - PS417_23665 0.46 +2.1 5,117,691 - PS417_23665 0.46 +0.3 5,117,861 + +0.6 5,117,862 - -0.2 5,118,309 + PS417_23670 0.40 +1.8 5,118,310 - PS417_23670 0.40 -0.0 5,118,310 - PS417_23670 0.40 +0.4 5,118,696 + PS417_23670 0.74 -2.4 5,118,697 - PS417_23670 0.74 -0.2 5,118,789 + PS417_23670 0.82 +2.5 5,118,813 - -1.0
Or see this region's nucleotide sequence