Strain Fitness in Pseudomonas simiae WCS417 around PS417_23660

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_23650 and PS417_23655 are separated by 144 nucleotidesPS417_23655 and PS417_23660 are separated by 104 nucleotidesPS417_23660 and PS417_23665 are separated by 41 nucleotidesPS417_23665 and PS417_23670 are separated by 12 nucleotides PS417_23650: PS417_23650 - Kynureninase (EC 3.7.1.3) (from data), at 5,114,613 to 5,115,863 _23650 PS417_23655: PS417_23655 - AsnC family transcriptional regulator, at 5,116,008 to 5,116,481 _23655 PS417_23660: PS417_23660 - Catechol 1,2-dioxygenase (EC 1.13.11.1) (from data), at 5,116,586 to 5,117,515 _23660 PS417_23665: PS417_23665 - Muconolactone isomerase (EC 5.3.3.4) (from data), at 5,117,557 to 5,117,847 _23665 PS417_23670: PS417_23670 - Muconate cycloisomerase (EC 5.5.1.1) (from data), at 5,117,860 to 5,118,987 _23670 Position (kb) 5116 5117 5118Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5115.678 kb on - strand, within PS417_23650at 5115.772 kb on + strandat 5115.773 kb on - strandat 5115.886 kb on + strandat 5115.956 kb on - strandat 5116.003 kb on + strandat 5116.299 kb on + strand, within PS417_23655at 5116.300 kb on - strand, within PS417_23655at 5116.323 kb on + strand, within PS417_23655at 5116.323 kb on + strand, within PS417_23655at 5116.323 kb on + strand, within PS417_23655at 5116.323 kb on + strand, within PS417_23655at 5116.323 kb on + strand, within PS417_23655at 5116.324 kb on - strand, within PS417_23655at 5116.324 kb on - strand, within PS417_23655at 5116.324 kb on - strand, within PS417_23655at 5116.410 kb on - strand, within PS417_23655at 5116.411 kb on + strand, within PS417_23655at 5116.411 kb on + strand, within PS417_23655at 5116.411 kb on + strand, within PS417_23655at 5116.411 kb on + strand, within PS417_23655at 5116.412 kb on - strand, within PS417_23655at 5116.528 kb on + strandat 5116.529 kb on - strandat 5116.587 kb on + strandat 5117.029 kb on - strand, within PS417_23660at 5117.029 kb on - strand, within PS417_23660at 5117.029 kb on - strand, within PS417_23660at 5117.032 kb on + strand, within PS417_23660at 5117.187 kb on + strand, within PS417_23660at 5117.187 kb on + strand, within PS417_23660at 5117.187 kb on + strand, within PS417_23660at 5117.188 kb on - strand, within PS417_23660at 5117.188 kb on - strand, within PS417_23660at 5117.188 kb on - strand, within PS417_23660at 5117.188 kb on - strand, within PS417_23660at 5117.268 kb on + strand, within PS417_23660at 5117.269 kb on - strand, within PS417_23660at 5117.322 kb on - strand, within PS417_23660at 5117.334 kb on + strandat 5117.335 kb on - strand, within PS417_23660at 5117.335 kb on - strand, within PS417_23660at 5117.335 kb on - strand, within PS417_23660at 5117.335 kb on - strand, within PS417_23660at 5117.535 kb on - strandat 5117.538 kb on + strandat 5117.538 kb on + strandat 5117.539 kb on - strandat 5117.539 kb on - strandat 5117.539 kb on - strandat 5117.539 kb on - strandat 5117.541 kb on + strandat 5117.541 kb on + strandat 5117.541 kb on + strandat 5117.621 kb on + strand, within PS417_23665at 5117.621 kb on + strand, within PS417_23665at 5117.621 kb on + strand, within PS417_23665at 5117.691 kb on - strand, within PS417_23665at 5117.691 kb on - strand, within PS417_23665at 5117.691 kb on - strand, within PS417_23665at 5117.861 kb on + strandat 5117.862 kb on - strandat 5118.309 kb on + strand, within PS417_23670at 5118.310 kb on - strand, within PS417_23670at 5118.310 kb on - strand, within PS417_23670

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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5,115,678 - PS417_23650 0.85 -0.3
5,115,772 + -4.2
5,115,773 - +0.9
5,115,886 + -1.1
5,115,956 - -1.2
5,116,003 + -0.4
5,116,299 + PS417_23655 0.61 -1.0
5,116,300 - PS417_23655 0.62 +0.6
5,116,323 + PS417_23655 0.66 -2.0
5,116,323 + PS417_23655 0.66 -1.7
5,116,323 + PS417_23655 0.66 -0.3
5,116,323 + PS417_23655 0.66 +0.3
5,116,323 + PS417_23655 0.66 -1.9
5,116,324 - PS417_23655 0.67 +0.2
5,116,324 - PS417_23655 0.67 +0.5
5,116,324 - PS417_23655 0.67 -1.9
5,116,410 - PS417_23655 0.85 +0.6
5,116,411 + PS417_23655 0.85 +1.6
5,116,411 + PS417_23655 0.85 -0.3
5,116,411 + PS417_23655 0.85 +1.1
5,116,411 + PS417_23655 0.85 -1.1
5,116,412 - PS417_23655 0.85 +0.6
5,116,528 + +2.5
5,116,529 - -2.4
5,116,587 + +1.6
5,117,029 - PS417_23660 0.48 +0.4
5,117,029 - PS417_23660 0.48 -3.4
5,117,029 - PS417_23660 0.48 +0.5
5,117,032 + PS417_23660 0.48 -0.3
5,117,187 + PS417_23660 0.65 -1.2
5,117,187 + PS417_23660 0.65 -0.5
5,117,187 + PS417_23660 0.65 -1.0
5,117,188 - PS417_23660 0.65 -2.6
5,117,188 - PS417_23660 0.65 -2.0
5,117,188 - PS417_23660 0.65 +0.0
5,117,188 - PS417_23660 0.65 -3.1
5,117,268 + PS417_23660 0.73 +0.5
5,117,269 - PS417_23660 0.73 +2.0
5,117,322 - PS417_23660 0.79 -1.8
5,117,334 + -0.2
5,117,335 - PS417_23660 0.81 -0.0
5,117,335 - PS417_23660 0.81 -2.9
5,117,335 - PS417_23660 0.81 -0.1
5,117,335 - PS417_23660 0.81 +0.2
5,117,535 - +1.6
5,117,538 + -0.3
5,117,538 + +0.4
5,117,539 - +0.3
5,117,539 - +1.1
5,117,539 - -0.8
5,117,539 - -0.3
5,117,541 + -1.1
5,117,541 + +1.5
5,117,541 + -1.9
5,117,621 + PS417_23665 0.22 +0.0
5,117,621 + PS417_23665 0.22 +0.0
5,117,621 + PS417_23665 0.22 +0.8
5,117,691 - PS417_23665 0.46 +2.0
5,117,691 - PS417_23665 0.46 +2.1
5,117,691 - PS417_23665 0.46 +0.3
5,117,861 + +0.6
5,117,862 - -0.2
5,118,309 + PS417_23670 0.40 +1.8
5,118,310 - PS417_23670 0.40 -0.0
5,118,310 - PS417_23670 0.40 +0.4

Or see this region's nucleotide sequence