Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23110 and PS417_23115 are separated by 176 nucleotides PS417_23115 and PS417_23120 overlap by 13 nucleotides
PS417_23110: PS417_23110 - 2-isopropylmalate synthase, at 5,008,765 to 5,010,444
_23110
PS417_23115: PS417_23115 - carbon-nitrogen hydrolase, at 5,010,621 to 5,011,412
_23115
PS417_23120: PS417_23120 - aminotransferase, at 5,011,400 to 5,012,548
_23120
Position (kb)
5010
5011
5012 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5009.641 kb on + strand at 5009.641 kb on + strand, within PS417_23110 at 5009.641 kb on + strand, within PS417_23110 at 5009.642 kb on - strand, within PS417_23110 at 5009.642 kb on - strand, within PS417_23110 at 5009.642 kb on - strand, within PS417_23110 at 5009.642 kb on - strand, within PS417_23110 at 5010.050 kb on - strand, within PS417_23110 at 5010.070 kb on + strand, within PS417_23110 at 5010.084 kb on + strand, within PS417_23110 at 5010.259 kb on + strand, within PS417_23110 at 5010.259 kb on + strand, within PS417_23110 at 5010.259 kb on + strand, within PS417_23110 at 5010.308 kb on - strand at 5010.458 kb on + strand at 5010.502 kb on - strand at 5010.561 kb on + strand at 5010.638 kb on + strand at 5010.829 kb on + strand, within PS417_23115 at 5010.829 kb on + strand, within PS417_23115 at 5010.965 kb on - strand, within PS417_23115 at 5011.174 kb on + strand, within PS417_23115 at 5011.175 kb on - strand, within PS417_23115 at 5011.216 kb on + strand, within PS417_23115 at 5011.412 kb on + strand at 5011.497 kb on + strand at 5011.590 kb on + strand, within PS417_23120 at 5011.609 kb on - strand, within PS417_23120 at 5011.628 kb on - strand, within PS417_23120 at 5011.776 kb on + strand, within PS417_23120 at 5011.776 kb on + strand, within PS417_23120 at 5011.776 kb on + strand, within PS417_23120 at 5011.777 kb on - strand, within PS417_23120 at 5011.777 kb on - strand, within PS417_23120 at 5011.827 kb on + strand, within PS417_23120 at 5011.828 kb on - strand, within PS417_23120 at 5011.854 kb on + strand, within PS417_23120 at 5011.854 kb on + strand, within PS417_23120 at 5011.855 kb on - strand, within PS417_23120 at 5011.940 kb on + strand, within PS417_23120 at 5011.974 kb on + strand, within PS417_23120 at 5011.974 kb on + strand, within PS417_23120 at 5011.975 kb on - strand, within PS417_23120 at 5012.184 kb on + strand, within PS417_23120 at 5012.184 kb on + strand, within PS417_23120 at 5012.184 kb on + strand, within PS417_23120 at 5012.185 kb on - strand, within PS417_23120 at 5012.185 kb on - strand, within PS417_23120 at 5012.185 kb on - strand, within PS417_23120 at 5012.185 kb on - strand, within PS417_23120 at 5012.185 kb on - strand, within PS417_23120 at 5012.193 kb on + strand, within PS417_23120 at 5012.194 kb on - strand, within PS417_23120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,009,641 + -0.5 5,009,641 + PS417_23110 0.52 -1.3 5,009,641 + PS417_23110 0.52 +0.1 5,009,642 - PS417_23110 0.52 +1.5 5,009,642 - PS417_23110 0.52 -3.2 5,009,642 - PS417_23110 0.52 -2.0 5,009,642 - PS417_23110 0.52 +0.1 5,010,050 - PS417_23110 0.76 +0.9 5,010,070 + PS417_23110 0.78 +0.7 5,010,084 + PS417_23110 0.79 -0.1 5,010,259 + PS417_23110 0.89 +0.7 5,010,259 + PS417_23110 0.89 +0.2 5,010,259 + PS417_23110 0.89 -0.1 5,010,308 - -0.5 5,010,458 + +0.0 5,010,502 - +0.1 5,010,561 + +0.6 5,010,638 + -0.0 5,010,829 + PS417_23115 0.26 +0.8 5,010,829 + PS417_23115 0.26 +0.3 5,010,965 - PS417_23115 0.43 +1.6 5,011,174 + PS417_23115 0.70 +0.8 5,011,175 - PS417_23115 0.70 +0.2 5,011,216 + PS417_23115 0.75 -0.3 5,011,412 + +0.4 5,011,497 + +1.2 5,011,590 + PS417_23120 0.17 +0.2 5,011,609 - PS417_23120 0.18 +0.1 5,011,628 - PS417_23120 0.20 -2.0 5,011,776 + PS417_23120 0.33 -0.5 5,011,776 + PS417_23120 0.33 -0.6 5,011,776 + PS417_23120 0.33 -1.2 5,011,777 - PS417_23120 0.33 +0.0 5,011,777 - PS417_23120 0.33 +0.1 5,011,827 + PS417_23120 0.37 +0.2 5,011,828 - PS417_23120 0.37 -3.5 5,011,854 + PS417_23120 0.40 -2.3 5,011,854 + PS417_23120 0.40 -0.2 5,011,855 - PS417_23120 0.40 -2.1 5,011,940 + PS417_23120 0.47 -0.2 5,011,974 + PS417_23120 0.50 -0.6 5,011,974 + PS417_23120 0.50 +0.3 5,011,975 - PS417_23120 0.50 +0.1 5,012,184 + PS417_23120 0.68 -0.3 5,012,184 + PS417_23120 0.68 -2.2 5,012,184 + PS417_23120 0.68 -0.3 5,012,185 - PS417_23120 0.68 -0.0 5,012,185 - PS417_23120 0.68 -1.8 5,012,185 - PS417_23120 0.68 +0.1 5,012,185 - PS417_23120 0.68 +1.1 5,012,185 - PS417_23120 0.68 -1.2 5,012,193 + PS417_23120 0.69 +0.5 5,012,194 - PS417_23120 0.69 +1.7
Or see this region's nucleotide sequence