Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22785 and PS417_22790 are separated by 50 nucleotides PS417_22790 and PS417_22795 are separated by 246 nucleotides PS417_22795 and PS417_22800 are separated by 66 nucleotides
PS417_22785: PS417_22785 - molecular chaperone GroEL, at 4,937,405 to 4,939,051
_22785
PS417_22790: PS417_22790 - molecular chaperone GroES, at 4,939,102 to 4,939,398
_22790
PS417_22795: PS417_22795 - exclusion suppressor FxsA, at 4,939,645 to 4,940,121
_22795
PS417_22800: PS417_22800 - heme iron utilization protein, at 4,940,188 to 4,940,919
_22800
Position (kb)
4939
4940 Strain fitness (log2 ratio)
-2
-1
0
1 at 4939.539 kb on - strand at 4939.553 kb on - strand at 4939.553 kb on - strand at 4939.562 kb on - strand at 4939.562 kb on - strand at 4939.603 kb on + strand at 4939.843 kb on + strand, within PS417_22795 at 4940.069 kb on - strand, within PS417_22795 at 4940.163 kb on + strand at 4940.164 kb on - strand at 4940.164 kb on - strand at 4940.254 kb on + strand at 4940.297 kb on + strand, within PS417_22800 at 4940.297 kb on + strand, within PS417_22800 at 4940.299 kb on + strand, within PS417_22800 at 4940.313 kb on + strand, within PS417_22800 at 4940.390 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,939,539 - +0.1 4,939,553 - -1.8 4,939,553 - -1.9 4,939,562 - -0.2 4,939,562 - -2.7 4,939,603 + -0.4 4,939,843 + PS417_22795 0.42 +0.8 4,940,069 - PS417_22795 0.89 +0.4 4,940,163 + -0.9 4,940,164 - +0.0 4,940,164 - -0.1 4,940,254 + +0.4 4,940,297 + PS417_22800 0.15 +0.5 4,940,297 + PS417_22800 0.15 +0.8 4,940,299 + PS417_22800 0.15 -1.2 4,940,313 + PS417_22800 0.17 -1.5 4,940,390 + -1.2
Or see this region's nucleotide sequence