Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02265 and PS417_02270 are separated by 675 nucleotides PS417_02270 and PS417_02275 are separated by 0 nucleotides PS417_02275 and PS417_02280 overlap by 4 nucleotides
PS417_02265: PS417_02265 - TonB-dependent receptor, at 515,415 to 517,499
_02265
PS417_02270: PS417_02270 - carbamoyltransferase, at 518,175 to 519,932
_02270
PS417_02275: PS417_02275 - acetyltransferase, at 519,933 to 520,829
_02275
PS417_02280: PS417_02280 - GlcNAc-PI de-N-acetylase, at 520,826 to 522,226
_02280
Position (kb) 518
519
520 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 517.246 kb on + strand, within PS417_02265 at 517.247 kb on - strand, within PS417_02265 at 517.247 kb on - strand, within PS417_02265 at 517.464 kb on + strand at 517.532 kb on + strand at 517.533 kb on - strand at 517.631 kb on - strand at 517.633 kb on + strand at 517.633 kb on + strand at 517.672 kb on + strand at 517.689 kb on + strand at 517.689 kb on + strand at 517.705 kb on + strand at 517.706 kb on - strand at 517.714 kb on + strand at 517.715 kb on - strand at 517.746 kb on + strand at 517.746 kb on + strand at 517.747 kb on - strand at 517.994 kb on + strand at 517.995 kb on - strand at 518.027 kb on - strand at 518.079 kb on - strand at 518.147 kb on + strand at 518.147 kb on + strand at 518.156 kb on + strand at 518.156 kb on + strand at 518.235 kb on + strand at 518.721 kb on + strand, within PS417_02270 at 518.721 kb on + strand, within PS417_02270 at 518.922 kb on + strand, within PS417_02270 at 519.012 kb on + strand, within PS417_02270 at 519.012 kb on + strand, within PS417_02270 at 519.012 kb on + strand, within PS417_02270 at 519.240 kb on + strand, within PS417_02270 at 519.732 kb on + strand, within PS417_02270 at 520.642 kb on - strand, within PS417_02275 at 520.825 kb on + strand at 520.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 517,246 + PS417_02265 0.88 +0.7 517,247 - PS417_02265 0.88 -0.3 517,247 - PS417_02265 0.88 +0.1 517,464 + -2.6 517,532 + +0.1 517,533 - -0.9 517,631 - +1.0 517,633 + -2.0 517,633 + +1.3 517,672 + -0.7 517,689 + -1.2 517,689 + +0.3 517,705 + +0.0 517,706 - -1.4 517,714 + +3.7 517,715 - +0.3 517,746 + +0.5 517,746 + +4.1 517,747 - -2.5 517,994 + +1.2 517,995 - -0.8 518,027 - +2.8 518,079 - -1.4 518,147 + +1.3 518,147 + +0.4 518,156 + +3.0 518,156 + +2.6 518,235 + +5.9 518,721 + PS417_02270 0.31 +4.8 518,721 + PS417_02270 0.31 +3.7 518,922 + PS417_02270 0.42 +6.3 519,012 + PS417_02270 0.48 +5.0 519,012 + PS417_02270 0.48 +4.5 519,012 + PS417_02270 0.48 +5.5 519,240 + PS417_02270 0.61 +6.0 519,732 + PS417_02270 0.89 +5.4 520,642 - PS417_02275 0.79 -1.5 520,825 + +1.2 520,825 + +0.8
Or see this region's nucleotide sequence