Strain Fitness in Pseudomonas simiae WCS417 around PS417_22495

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_22485 and PS417_22490 are separated by 69 nucleotidesPS417_22490 and PS417_22495 are separated by 3 nucleotidesPS417_22495 and PS417_22500 are separated by 886 nucleotides PS417_22485: PS417_22485 - cold-shock protein, at 4,867,692 to 4,868,294 _22485 PS417_22490: PS417_22490 - hypothetical protein, at 4,868,364 to 4,868,570 _22490 PS417_22495: PS417_22495 - histidine triad (HIT) protein, at 4,868,574 to 4,868,999 _22495 PS417_22500: PS417_22500 - porin, at 4,869,886 to 4,871,184 _22500 Position (kb) 4868 4869Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4867.674 kb on + strandat 4868.362 kb on + strandat 4868.365 kb on + strandat 4868.366 kb on - strandat 4868.368 kb on + strandat 4868.368 kb on + strandat 4868.368 kb on + strandat 4868.369 kb on - strandat 4868.369 kb on - strandat 4868.654 kb on - strandat 4868.745 kb on + strand, within PS417_22495at 4868.745 kb on + strand, within PS417_22495at 4868.746 kb on - strand, within PS417_22495at 4868.988 kb on + strandat 4868.989 kb on - strandat 4869.364 kb on + strandat 4869.365 kb on - strandat 4869.418 kb on + strandat 4869.615 kb on - strandat 4869.718 kb on + strandat 4869.794 kb on - strandat 4869.794 kb on - strandat 4869.936 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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4,867,674 + +0.4
4,868,362 + +1.4
4,868,365 + -3.1
4,868,366 - -1.4
4,868,368 + +1.1
4,868,368 + +1.7
4,868,368 + -1.0
4,868,369 - -1.2
4,868,369 - -1.6
4,868,654 - -0.8
4,868,745 + PS417_22495 0.40 +1.6
4,868,745 + PS417_22495 0.40 +2.1
4,868,746 - PS417_22495 0.40 +1.6
4,868,988 + +1.7
4,868,989 - +1.8
4,869,364 + -1.4
4,869,365 - +0.0
4,869,418 + +0.3
4,869,615 - +3.5
4,869,718 + -0.0
4,869,794 - -0.9
4,869,794 - -1.0
4,869,936 + +0.2

Or see this region's nucleotide sequence