Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22480 and PS417_22485 are separated by 415 nucleotides PS417_22485 and PS417_22490 are separated by 69 nucleotides PS417_22490 and PS417_22495 are separated by 3 nucleotides
PS417_22480: PS417_22480 - ferritin, at 4,866,803 to 4,867,276
_22480
PS417_22485: PS417_22485 - cold-shock protein, at 4,867,692 to 4,868,294
_22485
PS417_22490: PS417_22490 - hypothetical protein, at 4,868,364 to 4,868,570
_22490
PS417_22495: PS417_22495 - histidine triad (HIT) protein, at 4,868,574 to 4,868,999
_22495
Position (kb)
4867
4868
4869 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4866.734 kb on + strand at 4866.956 kb on + strand, within PS417_22480 at 4866.957 kb on - strand, within PS417_22480 at 4866.957 kb on - strand, within PS417_22480 at 4867.026 kb on + strand, within PS417_22480 at 4867.027 kb on - strand, within PS417_22480 at 4867.035 kb on + strand, within PS417_22480 at 4867.036 kb on - strand, within PS417_22480 at 4867.036 kb on - strand, within PS417_22480 at 4867.110 kb on + strand, within PS417_22480 at 4867.111 kb on - strand, within PS417_22480 at 4867.174 kb on + strand, within PS417_22480 at 4867.189 kb on - strand, within PS417_22480 at 4867.193 kb on + strand, within PS417_22480 at 4867.193 kb on + strand, within PS417_22480 at 4867.193 kb on + strand, within PS417_22480 at 4867.193 kb on + strand, within PS417_22480 at 4867.193 kb on + strand, within PS417_22480 at 4867.193 kb on + strand, within PS417_22480 at 4867.193 kb on + strand, within PS417_22480 at 4867.194 kb on - strand, within PS417_22480 at 4867.194 kb on - strand, within PS417_22480 at 4867.194 kb on - strand, within PS417_22480 at 4867.194 kb on - strand, within PS417_22480 at 4867.194 kb on - strand, within PS417_22480 at 4867.194 kb on - strand, within PS417_22480 at 4867.194 kb on - strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.197 kb on + strand, within PS417_22480 at 4867.198 kb on - strand, within PS417_22480 at 4867.198 kb on - strand, within PS417_22480 at 4867.198 kb on - strand, within PS417_22480 at 4867.198 kb on - strand, within PS417_22480 at 4867.198 kb on - strand, within PS417_22480 at 4867.198 kb on - strand, within PS417_22480 at 4867.198 kb on - strand, within PS417_22480 at 4867.280 kb on + strand at 4867.329 kb on - strand at 4867.674 kb on + strand at 4868.362 kb on + strand at 4868.365 kb on + strand at 4868.366 kb on - strand at 4868.368 kb on + strand at 4868.368 kb on + strand at 4868.368 kb on + strand at 4868.369 kb on - strand at 4868.369 kb on - strand at 4868.654 kb on - strand at 4868.745 kb on + strand, within PS417_22495 at 4868.745 kb on + strand, within PS417_22495 at 4868.746 kb on - strand, within PS417_22495 at 4868.988 kb on + strand at 4868.989 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,866,734 + -0.2 4,866,956 + PS417_22480 0.32 +0.1 4,866,957 - PS417_22480 0.32 -0.3 4,866,957 - PS417_22480 0.32 -0.7 4,867,026 + PS417_22480 0.47 -0.3 4,867,027 - PS417_22480 0.47 -0.4 4,867,035 + PS417_22480 0.49 +1.1 4,867,036 - PS417_22480 0.49 -0.2 4,867,036 - PS417_22480 0.49 +0.3 4,867,110 + PS417_22480 0.65 +2.7 4,867,111 - PS417_22480 0.65 -0.1 4,867,174 + PS417_22480 0.78 +1.0 4,867,189 - PS417_22480 0.81 -0.7 4,867,193 + PS417_22480 0.82 +0.0 4,867,193 + PS417_22480 0.82 +0.7 4,867,193 + PS417_22480 0.82 +1.5 4,867,193 + PS417_22480 0.82 +0.0 4,867,193 + PS417_22480 0.82 -0.3 4,867,193 + PS417_22480 0.82 -0.1 4,867,193 + PS417_22480 0.82 +0.1 4,867,194 - PS417_22480 0.82 -0.4 4,867,194 - PS417_22480 0.82 +0.5 4,867,194 - PS417_22480 0.82 -1.4 4,867,194 - PS417_22480 0.82 +0.6 4,867,194 - PS417_22480 0.82 -0.1 4,867,194 - PS417_22480 0.82 -2.9 4,867,194 - PS417_22480 0.82 -0.1 4,867,197 + PS417_22480 0.83 -0.0 4,867,197 + PS417_22480 0.83 -0.3 4,867,197 + PS417_22480 0.83 -2.5 4,867,197 + PS417_22480 0.83 +0.2 4,867,197 + PS417_22480 0.83 -1.4 4,867,197 + PS417_22480 0.83 -1.9 4,867,197 + PS417_22480 0.83 +0.7 4,867,197 + PS417_22480 0.83 +0.5 4,867,197 + PS417_22480 0.83 +0.3 4,867,197 + PS417_22480 0.83 -0.1 4,867,197 + PS417_22480 0.83 -2.5 4,867,197 + PS417_22480 0.83 +0.4 4,867,197 + PS417_22480 0.83 -0.1 4,867,197 + PS417_22480 0.83 -0.5 4,867,197 + PS417_22480 0.83 -2.5 4,867,197 + -1.2 4,867,197 + PS417_22480 0.83 -0.1 4,867,197 + PS417_22480 0.83 +0.2 4,867,197 + PS417_22480 0.83 -3.4 4,867,198 - PS417_22480 0.83 -1.3 4,867,198 - PS417_22480 0.83 +1.0 4,867,198 - PS417_22480 0.83 +0.8 4,867,198 - PS417_22480 0.83 +0.3 4,867,198 - PS417_22480 0.83 -0.5 4,867,198 - PS417_22480 0.83 -0.5 4,867,198 - PS417_22480 0.83 +0.7 4,867,280 + +0.0 4,867,329 - -1.3 4,867,674 + +0.4 4,868,362 + +1.4 4,868,365 + -3.1 4,868,366 - -1.4 4,868,368 + +1.1 4,868,368 + +1.7 4,868,368 + -1.0 4,868,369 - -1.2 4,868,369 - -1.6 4,868,654 - -0.8 4,868,745 + PS417_22495 0.40 +1.6 4,868,745 + PS417_22495 0.40 +2.1 4,868,746 - PS417_22495 0.40 +1.6 4,868,988 + +1.7 4,868,989 - +1.8
Or see this region's nucleotide sequence