Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22275 and PS417_22280 are separated by 87 nucleotides PS417_22280 and PS417_22285 are separated by 1 nucleotides PS417_22285 and PS417_22290 are separated by 106 nucleotides PS417_22290 and PS417_22295 are separated by 7 nucleotides
PS417_22275: PS417_22275 - phosphoesterase, at 4,826,366 to 4,827,229
_22275
PS417_22280: PS417_22280 - septation protein A, at 4,827,317 to 4,827,913
_22280
PS417_22285: PS417_22285 - hypothetical protein, at 4,827,915 to 4,828,214
_22285
PS417_22290: PS417_22290 - translation initiation factor 2, at 4,828,321 to 4,828,728
_22290
PS417_22295: PS417_22295 - chemotaxis protein CheY, at 4,828,736 to 4,829,413
_22295
Position (kb) 4827
4828
4829 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4826.915 kb on + strand, within PS417_22275 at 4826.997 kb on + strand, within PS417_22275 at 4827.239 kb on + strand at 4827.282 kb on + strand at 4827.282 kb on + strand at 4827.283 kb on - strand at 4827.285 kb on + strand at 4827.285 kb on + strand at 4827.922 kb on - strand at 4828.152 kb on + strand, within PS417_22285 at 4828.152 kb on + strand, within PS417_22285 at 4828.153 kb on - strand, within PS417_22285 at 4828.153 kb on - strand, within PS417_22285 at 4828.153 kb on - strand, within PS417_22285 at 4828.171 kb on - strand, within PS417_22285 at 4828.247 kb on + strand at 4828.297 kb on - strand at 4828.297 kb on - strand at 4828.329 kb on - strand at 4828.329 kb on - strand at 4828.355 kb on + strand at 4828.519 kb on - strand, within PS417_22290 at 4828.755 kb on + strand at 4828.755 kb on + strand at 4828.756 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,826,915 + PS417_22275 0.64 -3.3 4,826,997 + PS417_22275 0.73 +0.5 4,827,239 + +0.7 4,827,282 + -1.8 4,827,282 + -1.7 4,827,283 - +0.2 4,827,285 + +0.3 4,827,285 + +0.6 4,827,922 - -1.6 4,828,152 + PS417_22285 0.79 -0.4 4,828,152 + PS417_22285 0.79 +0.5 4,828,153 - PS417_22285 0.79 -1.3 4,828,153 - PS417_22285 0.79 -1.2 4,828,153 - PS417_22285 0.79 -1.0 4,828,171 - PS417_22285 0.85 -0.8 4,828,247 + +1.2 4,828,297 - -1.7 4,828,297 - +0.4 4,828,329 - +0.3 4,828,329 - +0.2 4,828,355 + +0.2 4,828,519 - PS417_22290 0.49 +1.0 4,828,755 + -2.4 4,828,755 + -3.2 4,828,756 - -1.5
Or see this region's nucleotide sequence