Strain Fitness in Pseudomonas simiae WCS417 around PS417_22195

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.003 mg/ml
4,806,880	+		PS417_22190	0.60	+1.1
4,806,881	-		PS417_22190	0.60	+0.6
4,806,974	-		PS417_22190	0.65	+1.0
4,807,320	+				+0.8
4,807,524	+				+0.5
4,807,582	+				-1.5
4,807,583	-				+1.2
4,807,583	-				+0.8
4,807,583	-				+2.2
4,807,583	-				-1.4
4,807,624	+				+0.2
4,807,734	-				+0.8
4,807,837	+				-0.9
4,807,838	-				-3.4
4,808,163	-		PS417_22195	0.25	-3.0
4,808,163	-		PS417_22195	0.25	-0.5
4,808,312	-		PS417_22195	0.36	-1.0
4,808,513	+		PS417_22195	0.51	-2.7
4,808,646	-		PS417_22195	0.61	-1.7
4,808,822	+		PS417_22195	0.74	-1.4
4,809,017	+		PS417_22195	0.88	-0.3
4,809,188	+				-0.8
4,809,266	+				-1.8
4,809,305	+				-0.8
4,809,305	+				-0.1
4,809,329	+				+0.4
4,809,330	-				-1.4
4,809,330	-				-0.4
4,809,388	+				+0.0
4,809,426	+				-1.3
4,809,503	-				-4.1
4,809,700	-		PS417_22200	0.11	-1.0
4,809,727	+		PS417_22200	0.11	+0.7
4,809,727	+		PS417_22200	0.11	-1.5
4,809,728	-		PS417_22200	0.11	+0.2
4,809,728	-		PS417_22200	0.11	-1.7
4,809,728	-		PS417_22200	0.11	+0.8
4,809,883	+				-1.2
4,809,883	+		PS417_22200	0.15	-0.5
4,809,883	+		PS417_22200	0.15	-0.9
4,809,884	-		PS417_22200	0.15	-0.1
4,809,884	-		PS417_22200	0.15	+0.8
4,809,884	-		PS417_22200	0.15	+0.9
4,809,884	-		PS417_22200	0.15	+1.5
4,809,884	-		PS417_22200	0.15	-0.1
4,809,884	-		PS417_22200	0.15	-0.7
4,809,973	+				+0.8
4,809,974	-		PS417_22200	0.17	-0.5
4,809,974	-		PS417_22200	0.17	-1.3
4,809,974	-		PS417_22200	0.17	+0.9
4,810,012	+		PS417_22200	0.18	+1.1
4,810,013	-		PS417_22200	0.18	-0.4
4,810,044	+		PS417_22200	0.19	-2.0
4,810,045	-		PS417_22200	0.19	-2.5
4,810,045	-		PS417_22200	0.19	+0.4
4,810,108	+		PS417_22200	0.20	+0.3
4,810,108	+		PS417_22200	0.20	-1.0
4,810,108	+				-1.7
4,810,108	+		PS417_22200	0.20	-0.1
4,810,109	-		PS417_22200	0.20	-2.7
4,810,109	-		PS417_22200	0.20	+0.6