Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22160 and PS417_22165 are separated by 52 nucleotides PS417_22165 and PS417_22170 are separated by 98 nucleotides PS417_22170 and PS417_22175 are separated by 5 nucleotides
PS417_22160: PS417_22160 - 3-beta hydroxysteroid dehydrogenase, at 4,801,293 to 4,802,291
_22160
PS417_22165: PS417_22165 - LysR family transcriptional regulator, at 4,802,344 to 4,803,237
_22165
PS417_22170: PS417_22170 - acetyltransferase, at 4,803,336 to 4,803,737
_22170
PS417_22175: PS417_22175 - amino acid transporter LysE, at 4,803,743 to 4,804,342
_22175
Position (kb)
4802
4803
4804 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4801.372 kb on + strand at 4801.372 kb on + strand at 4801.511 kb on - strand, within PS417_22160 at 4801.570 kb on + strand, within PS417_22160 at 4801.570 kb on + strand, within PS417_22160 at 4801.570 kb on + strand, within PS417_22160 at 4801.571 kb on - strand, within PS417_22160 at 4801.571 kb on - strand, within PS417_22160 at 4801.619 kb on - strand, within PS417_22160 at 4801.945 kb on + strand, within PS417_22160 at 4801.946 kb on - strand, within PS417_22160 at 4802.051 kb on - strand, within PS417_22160 at 4802.051 kb on - strand, within PS417_22160 at 4802.051 kb on - strand, within PS417_22160 at 4802.290 kb on + strand at 4802.293 kb on + strand at 4802.293 kb on + strand at 4802.293 kb on + strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.297 kb on - strand at 4802.438 kb on + strand, within PS417_22165 at 4802.461 kb on - strand, within PS417_22165 at 4802.482 kb on + strand, within PS417_22165 at 4802.560 kb on - strand, within PS417_22165 at 4802.581 kb on + strand, within PS417_22165 at 4802.582 kb on - strand at 4802.582 kb on - strand, within PS417_22165 at 4802.624 kb on + strand, within PS417_22165 at 4803.226 kb on - strand at 4803.280 kb on - strand at 4803.321 kb on + strand at 4803.322 kb on - strand at 4803.323 kb on + strand at 4803.324 kb on - strand at 4803.324 kb on - strand at 4803.402 kb on + strand, within PS417_22170 at 4803.402 kb on + strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.654 kb on + strand at 4803.706 kb on + strand at 4803.706 kb on + strand at 4803.707 kb on - strand at 4803.736 kb on - strand at 4803.742 kb on + strand at 4803.743 kb on - strand at 4803.820 kb on - strand, within PS417_22175 at 4804.178 kb on + strand, within PS417_22175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,801,372 + -1.5 4,801,372 + +0.6 4,801,511 - PS417_22160 0.22 -2.4 4,801,570 + PS417_22160 0.28 -0.1 4,801,570 + PS417_22160 0.28 +0.3 4,801,570 + PS417_22160 0.28 +1.4 4,801,571 - PS417_22160 0.28 -0.1 4,801,571 - PS417_22160 0.28 -0.2 4,801,619 - PS417_22160 0.33 +0.1 4,801,945 + PS417_22160 0.65 -0.9 4,801,946 - PS417_22160 0.65 +1.1 4,802,051 - PS417_22160 0.76 +0.4 4,802,051 - PS417_22160 0.76 -0.8 4,802,051 - PS417_22160 0.76 +0.9 4,802,290 + +0.6 4,802,293 + +0.1 4,802,293 + -2.3 4,802,293 + +1.0 4,802,294 - +0.9 4,802,294 - -0.7 4,802,294 - -1.5 4,802,294 - -0.5 4,802,294 - -0.8 4,802,294 - +0.4 4,802,294 - +1.3 4,802,294 - +1.2 4,802,297 - -0.2 4,802,438 + PS417_22165 0.11 +1.4 4,802,461 - PS417_22165 0.13 -0.4 4,802,482 + PS417_22165 0.15 +2.6 4,802,560 - PS417_22165 0.24 +0.7 4,802,581 + PS417_22165 0.27 +1.2 4,802,582 - +4.7 4,802,582 - PS417_22165 0.27 -0.4 4,802,624 + PS417_22165 0.31 +0.9 4,803,226 - +2.3 4,803,280 - +0.7 4,803,321 + +1.0 4,803,322 - +1.7 4,803,323 + -1.8 4,803,324 - +2.6 4,803,324 - -2.7 4,803,402 + PS417_22170 0.16 -0.1 4,803,402 + PS417_22170 0.16 -3.5 4,803,403 - PS417_22170 0.17 +0.9 4,803,403 - PS417_22170 0.17 +1.6 4,803,654 + +1.8 4,803,706 + +0.3 4,803,706 + +2.6 4,803,707 - +0.3 4,803,736 - +1.0 4,803,742 + +0.5 4,803,743 - +0.8 4,803,820 - PS417_22175 0.13 +0.2 4,804,178 + PS417_22175 0.72 +2.8
Or see this region's nucleotide sequence