Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22050 and PS417_22055 are separated by 176 nucleotides PS417_22055 and PS417_22060 are separated by 199 nucleotides PS417_22060 and PS417_22065 are separated by 114 nucleotides
PS417_22050: PS417_22050 - ribonuclease BN, at 4,778,310 to 4,779,260
_22050
PS417_22055: PS417_22055 - hypothetical protein, at 4,779,437 to 4,779,628
_22055
PS417_22060: PS417_22060 - hypothetical protein, at 4,779,828 to 4,780,205
_22060
PS417_22065: PS417_22065 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (from data) , at 4,780,320 to 4,782,011
_22065
Position (kb)
4779
4780
4781 Strain fitness (log2 ratio)
-2
-1
0
1 at 4779.172 kb on + strand at 4779.173 kb on - strand at 4779.261 kb on - strand at 4779.333 kb on + strand at 4779.334 kb on - strand at 4779.334 kb on - strand at 4779.365 kb on + strand at 4779.631 kb on + strand at 4779.631 kb on + strand at 4779.667 kb on - strand at 4779.827 kb on + strand at 4779.981 kb on + strand, within PS417_22060 at 4780.089 kb on - strand, within PS417_22060 at 4780.316 kb on - strand at 4780.321 kb on + strand at 4780.669 kb on + strand, within PS417_22065 at 4780.759 kb on + strand, within PS417_22065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,779,172 + -0.9 4,779,173 - +1.4 4,779,261 - +1.4 4,779,333 + -1.5 4,779,334 - -0.5 4,779,334 - -1.9 4,779,365 + -2.5 4,779,631 + -2.1 4,779,631 + +0.1 4,779,667 - +0.1 4,779,827 + +0.4 4,779,981 + PS417_22060 0.40 -2.7 4,780,089 - PS417_22060 0.69 +0.2 4,780,316 - +0.8 4,780,321 + -0.4 4,780,669 + PS417_22065 0.21 -1.0 4,780,759 + PS417_22065 0.26 -2.0
Or see this region's nucleotide sequence