Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22025 and PS417_22030 are separated by 96 nucleotides PS417_22030 and PS417_22040 are separated by 239 nucleotides PS417_22040 and PS417_22045 are separated by 127 nucleotides PS417_22045 and PS417_22050 are separated by 3 nucleotides
PS417_22025: PS417_22025 - LysR family transcriptional regulator, at 4,775,385 to 4,776,254
_22025
PS417_22030: PS417_22030 - glutathione S-transferase, at 4,776,351 to 4,776,959
_22030
PS417_22040: PS417_22040 - GNAT family acetyltransferase, at 4,777,199 to 4,777,639
_22040
PS417_22045: PS417_22045 - peptide deformylase, at 4,777,767 to 4,778,306
_22045
PS417_22050: PS417_22050 - ribonuclease BN, at 4,778,310 to 4,779,260
_22050
Position (kb) 4777
4778 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4776.261 kb on - strand at 4776.261 kb on - strand at 4776.360 kb on + strand at 4776.360 kb on + strand at 4776.361 kb on - strand at 4776.395 kb on + strand at 4776.395 kb on + strand at 4776.501 kb on + strand, within PS417_22030 at 4776.502 kb on - strand, within PS417_22030 at 4776.502 kb on - strand, within PS417_22030 at 4776.564 kb on + strand, within PS417_22030 at 4776.783 kb on + strand, within PS417_22030 at 4776.784 kb on - strand, within PS417_22030 at 4776.819 kb on + strand, within PS417_22030 at 4776.820 kb on - strand, within PS417_22030 at 4777.084 kb on - strand at 4777.122 kb on + strand at 4777.122 kb on + strand at 4777.122 kb on + strand at 4777.122 kb on + strand at 4777.122 kb on + strand at 4777.123 kb on - strand at 4777.123 kb on - strand at 4777.123 kb on - strand at 4777.134 kb on + strand at 4777.134 kb on + strand at 4777.135 kb on - strand at 4777.135 kb on - strand at 4777.141 kb on + strand at 4777.141 kb on + strand at 4777.142 kb on - strand at 4777.162 kb on + strand at 4777.163 kb on - strand at 4777.163 kb on - strand at 4777.260 kb on - strand, within PS417_22040 at 4777.499 kb on + strand, within PS417_22040 at 4777.499 kb on + strand, within PS417_22040 at 4777.500 kb on - strand, within PS417_22040 at 4777.500 kb on - strand at 4777.565 kb on + strand, within PS417_22040 at 4777.565 kb on + strand, within PS417_22040 at 4777.849 kb on + strand, within PS417_22045 at 4777.850 kb on - strand, within PS417_22045 at 4778.530 kb on + strand, within PS417_22050 at 4778.530 kb on + strand, within PS417_22050 at 4778.530 kb on + strand, within PS417_22050 at 4778.531 kb on - strand, within PS417_22050 at 4778.531 kb on - strand, within PS417_22050 at 4778.531 kb on - strand, within PS417_22050 at 4778.531 kb on - strand, within PS417_22050 at 4778.563 kb on + strand, within PS417_22050 at 4778.564 kb on - strand, within PS417_22050 at 4778.564 kb on - strand, within PS417_22050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,776,261 - -0.3 4,776,261 - -1.0 4,776,360 + +1.5 4,776,360 + -0.3 4,776,361 - -1.1 4,776,395 + -2.5 4,776,395 + -0.1 4,776,501 + PS417_22030 0.25 +1.3 4,776,502 - PS417_22030 0.25 +1.2 4,776,502 - PS417_22030 0.25 +0.3 4,776,564 + PS417_22030 0.35 -0.7 4,776,783 + PS417_22030 0.71 +0.5 4,776,784 - PS417_22030 0.71 -0.5 4,776,819 + PS417_22030 0.77 +2.3 4,776,820 - PS417_22030 0.77 -0.1 4,777,084 - -0.9 4,777,122 + -1.9 4,777,122 + +2.2 4,777,122 + +0.7 4,777,122 + -0.0 4,777,122 + +0.8 4,777,123 - -0.2 4,777,123 - -0.5 4,777,123 - -0.2 4,777,134 + +1.0 4,777,134 + +0.3 4,777,135 - +1.0 4,777,135 - +0.2 4,777,141 + -0.6 4,777,141 + -1.4 4,777,142 - -1.4 4,777,162 + +0.9 4,777,163 - -0.0 4,777,163 - +0.2 4,777,260 - PS417_22040 0.14 -3.3 4,777,499 + PS417_22040 0.68 -2.7 4,777,499 + PS417_22040 0.68 +0.2 4,777,500 - PS417_22040 0.68 -2.5 4,777,500 - -0.2 4,777,565 + PS417_22040 0.83 -0.8 4,777,565 + PS417_22040 0.83 -2.9 4,777,849 + PS417_22045 0.15 -0.1 4,777,850 - PS417_22045 0.15 +0.0 4,778,530 + PS417_22050 0.23 -1.4 4,778,530 + PS417_22050 0.23 -0.8 4,778,530 + PS417_22050 0.23 +0.8 4,778,531 - PS417_22050 0.23 -3.9 4,778,531 - PS417_22050 0.23 +2.0 4,778,531 - PS417_22050 0.23 -1.0 4,778,531 - PS417_22050 0.23 +3.0 4,778,563 + PS417_22050 0.27 +0.4 4,778,564 - PS417_22050 0.27 -1.2 4,778,564 - PS417_22050 0.27 -2.5
Or see this region's nucleotide sequence