Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21105 and PS417_21110 are separated by 229 nucleotides PS417_21110 and PS417_21115 overlap by 1 nucleotides PS417_21115 and PS417_21120 are separated by 122 nucleotides PS417_21120 and PS417_21125 are separated by 152 nucleotides
PS417_21105: PS417_21105 - aminodeoxychorismate synthase, at 4,593,632 to 4,594,975
_21105
PS417_21110: PS417_21110 - hypothetical protein, at 4,595,205 to 4,595,507
_21110
PS417_21115: PS417_21115 - transcriptional regulator, at 4,595,507 to 4,595,863
_21115
PS417_21120: PS417_21120 - phosphoserine phosphatase, at 4,595,986 to 4,596,603
_21120
PS417_21125: PS417_21125 - phosphoadenosine phosphosulfate reductase, at 4,596,756 to 4,597,490
_21125
Position (kb) 4595
4596 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 4594.778 kb on + strand, within PS417_21105 at 4594.796 kb on + strand, within PS417_21105 at 4594.866 kb on + strand at 4594.867 kb on - strand at 4594.867 kb on - strand at 4594.954 kb on - strand at 4595.044 kb on - strand at 4595.128 kb on - strand at 4595.128 kb on - strand at 4595.133 kb on + strand at 4595.213 kb on - strand at 4595.237 kb on - strand, within PS417_21110 at 4595.237 kb on - strand, within PS417_21110 at 4595.259 kb on + strand, within PS417_21110 at 4595.292 kb on + strand, within PS417_21110 at 4595.348 kb on + strand, within PS417_21110 at 4595.361 kb on - strand, within PS417_21110 at 4595.452 kb on + strand, within PS417_21110 at 4595.452 kb on + strand, within PS417_21110 at 4595.452 kb on + strand, within PS417_21110 at 4595.453 kb on - strand at 4595.453 kb on - strand, within PS417_21110 at 4595.479 kb on - strand at 4595.521 kb on + strand at 4595.521 kb on + strand at 4595.523 kb on + strand at 4595.831 kb on - strand at 4595.831 kb on - strand at 4595.837 kb on + strand at 4595.838 kb on - strand at 4595.867 kb on + strand at 4595.868 kb on - strand at 4595.878 kb on + strand at 4596.045 kb on + strand at 4596.045 kb on + strand at 4596.045 kb on + strand at 4596.046 kb on - strand at 4596.143 kb on + strand, within PS417_21120 at 4596.144 kb on - strand, within PS417_21120 at 4596.144 kb on - strand, within PS417_21120 at 4596.173 kb on + strand, within PS417_21120 at 4596.231 kb on - strand, within PS417_21120 at 4596.488 kb on + strand, within PS417_21120 at 4596.488 kb on + strand, within PS417_21120 at 4596.489 kb on - strand, within PS417_21120 at 4596.507 kb on + strand, within PS417_21120 at 4596.620 kb on + strand at 4596.621 kb on - strand at 4596.702 kb on - strand at 4596.717 kb on + strand at 4596.717 kb on + strand at 4596.717 kb on + strand at 4596.718 kb on - strand at 4596.795 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,594,778 + PS417_21105 0.85 +0.4 4,594,796 + PS417_21105 0.87 -1.2 4,594,866 + -1.2 4,594,867 - -0.9 4,594,867 - -1.3 4,594,954 - +0.1 4,595,044 - -2.5 4,595,128 - -2.6 4,595,128 - -0.0 4,595,133 + +0.2 4,595,213 - -3.0 4,595,237 - PS417_21110 0.11 +1.7 4,595,237 - PS417_21110 0.11 -1.3 4,595,259 + PS417_21110 0.18 -3.1 4,595,292 + PS417_21110 0.29 +2.2 4,595,348 + PS417_21110 0.47 -0.2 4,595,361 - PS417_21110 0.51 +1.9 4,595,452 + PS417_21110 0.82 +1.5 4,595,452 + PS417_21110 0.82 -0.2 4,595,452 + PS417_21110 0.82 -0.4 4,595,453 - +0.8 4,595,453 - PS417_21110 0.82 +0.4 4,595,479 - -0.7 4,595,521 + -1.7 4,595,521 + -1.7 4,595,523 + -3.1 4,595,831 - +1.0 4,595,831 - +0.3 4,595,837 + -1.6 4,595,838 - -1.8 4,595,867 + -1.8 4,595,868 - +0.0 4,595,878 + +0.6 4,596,045 + +2.0 4,596,045 + -0.1 4,596,045 + +0.3 4,596,046 - +1.6 4,596,143 + PS417_21120 0.25 -0.3 4,596,144 - PS417_21120 0.26 -2.2 4,596,144 - PS417_21120 0.26 -0.6 4,596,173 + PS417_21120 0.30 +0.3 4,596,231 - PS417_21120 0.40 +0.0 4,596,488 + PS417_21120 0.81 +0.2 4,596,488 + PS417_21120 0.81 +6.0 4,596,489 - PS417_21120 0.81 -0.2 4,596,507 + PS417_21120 0.84 +1.1 4,596,620 + -0.2 4,596,621 - +1.2 4,596,702 - -1.2 4,596,717 + -2.9 4,596,717 + -0.3 4,596,717 + -2.1 4,596,718 - -1.6 4,596,795 + +0.2
Or see this region's nucleotide sequence