Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02065 and PS417_02070 overlap by 11 nucleotides PS417_02070 and PS417_02075 are separated by 29 nucleotides PS417_02075 and PS417_02080 overlap by 8 nucleotides
PS417_02065: PS417_02065 - membrane protein, at 462,463 to 464,775
_02065
PS417_02070: PS417_02070 - membrane protein, at 464,765 to 465,427
_02070
PS417_02075: PS417_02075 - thioesterase, at 465,457 to 465,882
_02075
PS417_02080: PS417_02080 - histidine ammonia-lyase, at 465,875 to 467,416
_02080
Position (kb) 464
465
466 Strain fitness (log2 ratio) -2
-1
0
1 at 463.811 kb on + strand, within PS417_02065 at 463.812 kb on - strand, within PS417_02065 at 463.835 kb on - strand, within PS417_02065 at 463.879 kb on - strand, within PS417_02065 at 463.982 kb on - strand, within PS417_02065 at 463.982 kb on - strand, within PS417_02065 at 464.083 kb on + strand, within PS417_02065 at 464.084 kb on - strand, within PS417_02065 at 464.573 kb on - strand at 464.578 kb on + strand at 464.610 kb on + strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.828 kb on - strand at 464.828 kb on - strand at 465.330 kb on - strand, within PS417_02070 at 465.476 kb on - strand at 465.596 kb on + strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 466.142 kb on + strand, within PS417_02080 at 466.142 kb on + strand, within PS417_02080 at 466.317 kb on + strand, within PS417_02080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 463,811 + PS417_02065 0.58 +0.6 463,812 - PS417_02065 0.58 -1.2 463,835 - PS417_02065 0.59 +0.8 463,879 - PS417_02065 0.61 +0.4 463,982 - PS417_02065 0.66 +0.6 463,982 - PS417_02065 0.66 -0.3 464,083 + PS417_02065 0.70 -0.0 464,084 - PS417_02065 0.70 +1.0 464,573 - +1.2 464,578 + +1.3 464,610 + -0.0 464,611 - -1.0 464,611 - -0.5 464,611 - -2.3 464,611 - +0.3 464,828 - +1.0 464,828 - -0.9 465,330 - PS417_02070 0.85 -2.3 465,476 - +0.9 465,596 + PS417_02075 0.33 +1.1 465,597 - PS417_02075 0.33 -2.7 465,597 - PS417_02075 0.33 +0.7 465,734 + PS417_02075 0.65 -0.4 465,734 + PS417_02075 0.65 -2.1 465,734 + PS417_02075 0.65 +1.1 465,735 - PS417_02075 0.65 -1.9 465,735 - PS417_02075 0.65 -1.1 466,142 + PS417_02080 0.17 -0.9 466,142 + PS417_02080 0.17 +0.7 466,317 + PS417_02080 0.29 -0.2
Or see this region's nucleotide sequence