Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19925 and PS417_19930 are separated by 132 nucleotides PS417_19930 and PS417_19935 are separated by 41 nucleotides PS417_19935 and PS417_19940 are separated by 84 nucleotides
PS417_19925: PS417_19925 - membrane protein, at 4,326,499 to 4,327,383
_19925
PS417_19930: PS417_19930 - DNA methyltransferase, at 4,327,516 to 4,327,797
_19930
PS417_19935: PS417_19935 - hypothetical protein, at 4,327,839 to 4,328,126
_19935
PS417_19940: PS417_19940 - membrane protein, at 4,328,211 to 4,330,400
_19940
Position (kb)
4327
4328
4329 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 4327.386 kb on + strand at 4327.389 kb on - strand at 4327.393 kb on + strand at 4327.393 kb on + strand at 4327.414 kb on - strand at 4327.512 kb on + strand at 4327.513 kb on - strand at 4327.513 kb on - strand at 4327.632 kb on - strand, within PS417_19930 at 4327.719 kb on + strand, within PS417_19930 at 4327.720 kb on - strand, within PS417_19930 at 4327.720 kb on - strand, within PS417_19930 at 4327.847 kb on + strand at 4327.893 kb on + strand at 4327.941 kb on - strand, within PS417_19935 at 4328.034 kb on - strand, within PS417_19935 at 4328.092 kb on + strand, within PS417_19935 at 4328.093 kb on - strand, within PS417_19935 at 4328.093 kb on - strand, within PS417_19935 at 4328.126 kb on + strand at 4328.126 kb on + strand at 4328.173 kb on - strand at 4328.174 kb on + strand at 4328.174 kb on + strand at 4328.175 kb on - strand at 4328.200 kb on - strand at 4328.672 kb on + strand, within PS417_19940 at 4328.672 kb on + strand, within PS417_19940 at 4328.672 kb on + strand, within PS417_19940 at 4328.673 kb on - strand, within PS417_19940 at 4328.768 kb on - strand, within PS417_19940 at 4328.960 kb on - strand, within PS417_19940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,327,386 + +0.2 4,327,389 - +1.1 4,327,393 + +0.2 4,327,393 + -0.4 4,327,414 - -2.1 4,327,512 + +0.3 4,327,513 - -0.1 4,327,513 - +1.7 4,327,632 - PS417_19930 0.41 -0.6 4,327,719 + PS417_19930 0.72 -0.9 4,327,720 - PS417_19930 0.72 +1.3 4,327,720 - PS417_19930 0.72 +0.9 4,327,847 + -2.2 4,327,893 + +0.1 4,327,941 - PS417_19935 0.35 +1.2 4,328,034 - PS417_19935 0.68 -2.7 4,328,092 + PS417_19935 0.88 +0.1 4,328,093 - PS417_19935 0.88 -1.8 4,328,093 - PS417_19935 0.88 -1.5 4,328,126 + -0.5 4,328,126 + -0.1 4,328,173 - +1.3 4,328,174 + +5.1 4,328,174 + +0.1 4,328,175 - -0.6 4,328,200 - +0.6 4,328,672 + PS417_19940 0.21 +0.4 4,328,672 + PS417_19940 0.21 +0.0 4,328,672 + PS417_19940 0.21 -0.1 4,328,673 - PS417_19940 0.21 +0.3 4,328,768 - PS417_19940 0.25 +0.2 4,328,960 - PS417_19940 0.34 -0.7
Or see this region's nucleotide sequence