Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01935 and PS417_01940 are separated by 216 nucleotides PS417_01940 and PS417_01945 are separated by 35 nucleotides PS417_01945 and PS417_01950 are separated by 181 nucleotides
PS417_01935: PS417_01935 - primosomal protein N', at 428,386 to 430,605
_01935
PS417_01940: PS417_01940 - 50S ribosomal protein L31, at 430,822 to 431,040
_01940
PS417_01945: PS417_01945 - nuclease, at 431,076 to 431,870
_01945
PS417_01950: PS417_01950 - malate dehydrogenase, at 432,052 to 433,320
_01950
Position (kb) 431
432 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 430.620 kb on - strand at 430.654 kb on + strand at 430.655 kb on - strand at 431.038 kb on + strand at 431.302 kb on - strand, within PS417_01945 at 431.419 kb on - strand, within PS417_01945 at 431.508 kb on - strand, within PS417_01945 at 431.922 kb on - strand at 431.935 kb on - strand at 431.955 kb on - strand at 432.007 kb on + strand at 432.035 kb on - strand at 432.051 kb on + strand at 432.051 kb on + strand at 432.116 kb on + strand at 432.117 kb on - strand at 432.117 kb on - strand at 432.163 kb on + strand at 432.164 kb on - strand at 432.227 kb on - strand, within PS417_01950 at 432.227 kb on - strand, within PS417_01950 at 432.327 kb on + strand, within PS417_01950 at 432.328 kb on - strand, within PS417_01950 at 432.697 kb on + strand, within PS417_01950 at 432.698 kb on - strand at 432.698 kb on - strand, within PS417_01950 at 432.751 kb on + strand, within PS417_01950
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 430,620 - +1.0 430,654 + -3.8 430,655 - -0.5 431,038 + +1.8 431,302 - PS417_01945 0.28 -0.1 431,419 - PS417_01945 0.43 -2.9 431,508 - PS417_01945 0.54 -2.2 431,922 - +0.0 431,935 - +0.0 431,955 - -0.9 432,007 + +0.3 432,035 - +0.5 432,051 + +1.6 432,051 + -0.0 432,116 + -2.5 432,117 - +1.4 432,117 - -0.8 432,163 + +0.0 432,164 - +0.5 432,227 - PS417_01950 0.14 +0.4 432,227 - PS417_01950 0.14 +0.6 432,327 + PS417_01950 0.22 -0.1 432,328 - PS417_01950 0.22 -2.9 432,697 + PS417_01950 0.51 -2.7 432,698 - +2.2 432,698 - PS417_01950 0.51 +1.0 432,751 + PS417_01950 0.55 -1.5
Or see this region's nucleotide sequence