Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19340 and PS417_19345 are separated by 75 nucleotides PS417_19345 and PS417_19350 are separated by 78 nucleotides PS417_19350 and PS417_19355 are separated by 101 nucleotides PS417_19355 and PS417_19360 are separated by 22 nucleotides PS417_19360 and PS417_19365 overlap by 4 nucleotides
PS417_19340: PS417_19340 - hypothetical protein, at 4,193,945 to 4,194,265
_19340
PS417_19345: PS417_19345 - DNA mismatch repair protein MutS, at 4,194,341 to 4,194,898
_19345
PS417_19350: PS417_19350 - GTP cyclohydrolase, at 4,194,977 to 4,195,522
_19350
PS417_19355: PS417_19355 - glutaredoxin, at 4,195,624 to 4,195,995
_19355
PS417_19360: PS417_19360 - hypothetical protein, at 4,196,018 to 4,196,338
_19360
PS417_19365: PS417_19365 - ABC transporter substrate-binding protein, at 4,196,335 to 4,197,660
_19365
Position (kb)
4194
4195
4196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4194.045 kb on + strand, within PS417_19340 at 4194.181 kb on - strand, within PS417_19340 at 4194.312 kb on + strand at 4194.313 kb on - strand at 4194.313 kb on - strand at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.854 kb on + strand at 4194.855 kb on - strand at 4194.855 kb on - strand at 4194.855 kb on - strand at 4194.855 kb on - strand at 4194.995 kb on + strand at 4194.996 kb on - strand at 4195.185 kb on - strand, within PS417_19350 at 4195.233 kb on + strand, within PS417_19350 at 4195.238 kb on + strand, within PS417_19350 at 4195.238 kb on + strand, within PS417_19350 at 4195.239 kb on - strand, within PS417_19350 at 4195.239 kb on - strand, within PS417_19350 at 4195.239 kb on - strand, within PS417_19350 at 4195.295 kb on + strand, within PS417_19350 at 4195.520 kb on + strand at 4195.584 kb on + strand at 4195.585 kb on - strand at 4195.912 kb on - strand, within PS417_19355 at 4195.942 kb on + strand, within PS417_19355 at 4195.942 kb on + strand at 4195.942 kb on + strand, within PS417_19355 at 4195.942 kb on + strand, within PS417_19355 at 4195.942 kb on + strand, within PS417_19355 at 4195.943 kb on - strand, within PS417_19355 at 4195.943 kb on - strand at 4195.943 kb on - strand at 4195.967 kb on - strand at 4196.002 kb on + strand at 4196.002 kb on + strand at 4196.020 kb on - strand at 4196.020 kb on - strand at 4196.250 kb on + strand, within PS417_19360 at 4196.281 kb on + strand, within PS417_19360 at 4196.282 kb on - strand, within PS417_19360 at 4196.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,194,045 + PS417_19340 0.31 +0.5 4,194,181 - PS417_19340 0.74 +2.4 4,194,312 + -1.4 4,194,313 - -1.3 4,194,313 - -2.5 4,194,854 + +0.2 4,194,854 + +1.2 4,194,854 + +0.9 4,194,854 + +0.8 4,194,854 + -0.1 4,194,855 - -3.0 4,194,855 - +0.9 4,194,855 - -0.7 4,194,855 - +0.1 4,194,995 + -0.3 4,194,996 - +0.7 4,195,185 - PS417_19350 0.38 -1.4 4,195,233 + PS417_19350 0.47 -1.4 4,195,238 + PS417_19350 0.48 -1.0 4,195,238 + PS417_19350 0.48 -2.2 4,195,239 - PS417_19350 0.48 -2.9 4,195,239 - PS417_19350 0.48 -0.6 4,195,239 - PS417_19350 0.48 -3.2 4,195,295 + PS417_19350 0.58 -2.6 4,195,520 + +2.6 4,195,584 + +0.0 4,195,585 - +0.8 4,195,912 - PS417_19355 0.77 -0.8 4,195,942 + PS417_19355 0.85 +0.7 4,195,942 + +2.5 4,195,942 + PS417_19355 0.85 +0.1 4,195,942 + PS417_19355 0.85 -2.7 4,195,942 + PS417_19355 0.85 +1.2 4,195,943 - PS417_19355 0.86 -1.4 4,195,943 - +3.3 4,195,943 - -1.3 4,195,967 - +0.4 4,196,002 + -0.6 4,196,002 + +0.6 4,196,020 - -0.6 4,196,020 - -2.1 4,196,250 + PS417_19360 0.72 +0.0 4,196,281 + PS417_19360 0.82 +0.0 4,196,282 - PS417_19360 0.82 +0.7 4,196,405 - -0.5
Or see this region's nucleotide sequence