Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19000 and PS417_19005 are separated by 121 nucleotides PS417_19005 and PS417_19010 overlap by 98 nucleotides PS417_19010 and PS417_19015 are separated by 9 nucleotides
PS417_19000: PS417_19000 - MFS transporter, at 4,112,285 to 4,113,502
_19000
PS417_19005: PS417_19005 - TetR family transcriptional regulator, at 4,113,624 to 4,114,328
_19005
PS417_19010: PS417_19010 - LysR family transcriptional regulator, at 4,114,231 to 4,115,154
_19010
PS417_19015: PS417_19015 - LysR family transcriptional regulator, at 4,115,164 to 4,116,060
_19015
Position (kb)
4114
4115
4116 Strain fitness (log2 ratio)
-1
0
1 at 4113.321 kb on + strand, within PS417_19000 at 4113.487 kb on + strand at 4113.488 kb on - strand at 4113.488 kb on - strand at 4113.597 kb on + strand at 4114.316 kb on + strand at 4114.316 kb on + strand at 4114.317 kb on - strand at 4114.317 kb on - strand at 4114.317 kb on - strand at 4114.327 kb on - strand at 4114.327 kb on - strand at 4114.410 kb on - strand, within PS417_19010 at 4114.621 kb on - strand, within PS417_19010 at 4114.625 kb on + strand, within PS417_19010 at 4114.721 kb on + strand, within PS417_19010 at 4114.832 kb on + strand, within PS417_19010 at 4114.833 kb on - strand, within PS417_19010 at 4115.262 kb on - strand, within PS417_19015 at 4115.262 kb on - strand, within PS417_19015 at 4115.262 kb on - strand, within PS417_19015 at 4115.348 kb on + strand, within PS417_19015 at 4115.348 kb on + strand, within PS417_19015 at 4115.454 kb on - strand, within PS417_19015 at 4115.498 kb on + strand, within PS417_19015 at 4115.612 kb on + strand, within PS417_19015 at 4115.613 kb on - strand, within PS417_19015 at 4115.712 kb on - strand, within PS417_19015 at 4115.825 kb on - strand, within PS417_19015 at 4115.825 kb on - strand, within PS417_19015 at 4116.069 kb on + strand at 4116.135 kb on - strand at 4116.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,113,321 + PS417_19000 0.85 +1.2 4,113,487 + -0.5 4,113,488 - +1.3 4,113,488 - +0.8 4,113,597 + +0.2 4,114,316 + +1.1 4,114,316 + +0.7 4,114,317 - +0.7 4,114,317 - +0.2 4,114,317 - +0.1 4,114,327 - +1.5 4,114,327 - -0.1 4,114,410 - PS417_19010 0.19 -0.7 4,114,621 - PS417_19010 0.42 -1.5 4,114,625 + PS417_19010 0.43 -0.7 4,114,721 + PS417_19010 0.53 -1.1 4,114,832 + PS417_19010 0.65 -0.0 4,114,833 - PS417_19010 0.65 -0.3 4,115,262 - PS417_19015 0.11 -1.2 4,115,262 - PS417_19015 0.11 +0.7 4,115,262 - PS417_19015 0.11 -1.5 4,115,348 + PS417_19015 0.21 +0.3 4,115,348 + PS417_19015 0.21 +0.9 4,115,454 - PS417_19015 0.32 +0.7 4,115,498 + PS417_19015 0.37 -1.0 4,115,612 + PS417_19015 0.50 +1.3 4,115,613 - PS417_19015 0.50 -1.0 4,115,712 - PS417_19015 0.61 -0.1 4,115,825 - PS417_19015 0.74 -0.1 4,115,825 - PS417_19015 0.74 +0.2 4,116,069 + +0.9 4,116,135 - +1.1 4,116,135 - -0.3
Or see this region's nucleotide sequence