Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18905 and PS417_18910 are separated by 165 nucleotides PS417_18910 and PS417_18915 overlap by 4 nucleotides PS417_18915 and PS417_18920 are separated by 9 nucleotides
PS417_18905: PS417_18905 - acyl-CoA dehydrogenase, at 4,094,498 to 4,095,739
_18905
PS417_18910: PS417_18910 - acetylornithine deacetylase, at 4,095,905 to 4,097,062
_18910
PS417_18915: PS417_18915 - hypothetical protein, at 4,097,059 to 4,097,733
_18915
PS417_18920: PS417_18920 - hypothetical protein, at 4,097,743 to 4,098,474
_18920
Position (kb)
4095
4096
4097
4098 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4094.996 kb on + strand, within PS417_18905 at 4095.172 kb on - strand, within PS417_18905 at 4095.441 kb on + strand, within PS417_18905 at 4095.441 kb on + strand at 4095.441 kb on + strand at 4095.656 kb on + strand at 4095.735 kb on + strand at 4095.735 kb on + strand at 4095.735 kb on + strand at 4095.736 kb on - strand at 4095.736 kb on - strand at 4095.874 kb on + strand at 4095.874 kb on + strand at 4095.899 kb on + strand at 4095.899 kb on + strand at 4095.901 kb on + strand at 4095.902 kb on - strand at 4095.902 kb on - strand at 4095.902 kb on - strand at 4095.906 kb on + strand at 4095.906 kb on + strand at 4095.982 kb on + strand at 4096.494 kb on + strand, within PS417_18910 at 4096.494 kb on + strand, within PS417_18910 at 4096.671 kb on + strand, within PS417_18910 at 4096.750 kb on - strand, within PS417_18910 at 4096.750 kb on - strand, within PS417_18910 at 4096.779 kb on + strand, within PS417_18910 at 4096.936 kb on - strand, within PS417_18910 at 4096.971 kb on + strand at 4096.972 kb on - strand at 4097.124 kb on - strand at 4097.385 kb on + strand, within PS417_18915 at 4097.386 kb on - strand, within PS417_18915 at 4097.821 kb on - strand, within PS417_18920 at 4097.821 kb on - strand, within PS417_18920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,094,996 + PS417_18905 0.40 +0.2 4,095,172 - PS417_18905 0.54 +1.2 4,095,441 + PS417_18905 0.76 +0.9 4,095,441 + +0.8 4,095,441 + +1.8 4,095,656 + +0.3 4,095,735 + -1.2 4,095,735 + -1.5 4,095,735 + -1.7 4,095,736 - -0.8 4,095,736 - -3.3 4,095,874 + +0.3 4,095,874 + +0.6 4,095,899 + +0.1 4,095,899 + -1.0 4,095,901 + -0.6 4,095,902 - -1.0 4,095,902 - -3.6 4,095,902 - +0.1 4,095,906 + -0.2 4,095,906 + -0.5 4,095,982 + -0.4 4,096,494 + PS417_18910 0.51 -1.0 4,096,494 + PS417_18910 0.51 -0.2 4,096,671 + PS417_18910 0.66 -0.2 4,096,750 - PS417_18910 0.73 +0.2 4,096,750 - PS417_18910 0.73 +0.2 4,096,779 + PS417_18910 0.75 +0.4 4,096,936 - PS417_18910 0.89 +0.0 4,096,971 + -2.3 4,096,972 - -0.5 4,097,124 - -2.7 4,097,385 + PS417_18915 0.48 -3.2 4,097,386 - PS417_18915 0.48 -0.0 4,097,821 - PS417_18920 0.11 -1.8 4,097,821 - PS417_18920 0.11 -2.8
Or see this region's nucleotide sequence