Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01870 and PS417_01875 are separated by 144 nucleotides PS417_01875 and PS417_01880 are separated by 105 nucleotides PS417_01880 and PS417_01885 are separated by 12 nucleotides
PS417_01870: PS417_01870 - ubiquinone biosynthesis methyltransferase UbiE, at 415,012 to 415,782
_01870
PS417_01875: PS417_01875 - polyhydroxyalkanoic acid system protein, at 415,927 to 416,202
_01875
PS417_01880: PS417_01880 - poly(3-hydroxyalkanoate) granule-associated protein PhaI, at 416,308 to 416,730
_01880
PS417_01885: PS417_01885 - poly(3-hydroxyalkanoate) granule-associated protein PhaF, at 416,743 to 417,726
_01885
Position (kb) 416
417 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 415.783 kb on - strand at 415.783 kb on - strand at 415.817 kb on - strand at 415.831 kb on - strand at 415.855 kb on + strand at 415.855 kb on + strand at 415.855 kb on + strand at 415.856 kb on - strand at 415.856 kb on - strand at 415.936 kb on - strand at 415.936 kb on - strand at 416.049 kb on + strand, within PS417_01875 at 416.087 kb on + strand, within PS417_01875 at 416.289 kb on - strand at 416.367 kb on + strand, within PS417_01880 at 416.368 kb on - strand, within PS417_01880 at 416.411 kb on - strand, within PS417_01880 at 416.547 kb on + strand, within PS417_01880 at 416.590 kb on - strand, within PS417_01880 at 416.705 kb on + strand at 416.705 kb on + strand at 416.706 kb on - strand at 416.706 kb on - strand at 416.706 kb on - strand at 416.721 kb on + strand at 416.728 kb on + strand at 416.728 kb on + strand at 416.760 kb on + strand at 416.869 kb on + strand, within PS417_01885 at 416.870 kb on - strand, within PS417_01885 at 416.870 kb on - strand, within PS417_01885 at 417.132 kb on + strand, within PS417_01885 at 417.621 kb on + strand, within PS417_01885 at 417.622 kb on - strand, within PS417_01885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 415,783 - -1.4 415,783 - -2.0 415,817 - +0.7 415,831 - -0.7 415,855 + -2.1 415,855 + +1.7 415,855 + +0.9 415,856 - +0.4 415,856 - +1.2 415,936 - +1.9 415,936 - -2.1 416,049 + PS417_01875 0.44 +1.1 416,087 + PS417_01875 0.58 -1.5 416,289 - +0.9 416,367 + PS417_01880 0.14 +0.5 416,368 - PS417_01880 0.14 +1.5 416,411 - PS417_01880 0.24 +0.1 416,547 + PS417_01880 0.57 -0.8 416,590 - PS417_01880 0.67 +1.0 416,705 + -1.0 416,705 + +0.9 416,706 - +0.9 416,706 - +0.2 416,706 - +0.5 416,721 + +0.2 416,728 + -0.8 416,728 + -1.4 416,760 + +0.4 416,869 + PS417_01885 0.13 -1.7 416,870 - PS417_01885 0.13 +0.8 416,870 - PS417_01885 0.13 +0.3 417,132 + PS417_01885 0.40 -3.0 417,621 + PS417_01885 0.89 +0.8 417,622 - PS417_01885 0.89 +0.5
Or see this region's nucleotide sequence