Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17900 and PS417_17905 are separated by 186 nucleotides PS417_17905 and PS417_17910 overlap by 38 nucleotides PS417_17910 and PS417_17915 are separated by 217 nucleotides
PS417_17900: PS417_17900 - transcriptional regulator, at 3,906,013 to 3,906,954
_17900
PS417_17905: PS417_17905 - hypothetical protein, at 3,907,141 to 3,907,707
_17905
PS417_17910: PS417_17910 - APC family amino acid permease, at 3,907,670 to 3,909,169
_17910
PS417_17915: PS417_17915 - hypothetical protein, at 3,909,387 to 3,910,709
_17915
Position (kb)
3907
3908
3909
3910 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3906.901 kb on + strand at 3906.901 kb on + strand at 3907.013 kb on + strand at 3907.049 kb on + strand at 3907.049 kb on + strand at 3907.056 kb on - strand at 3907.116 kb on - strand at 3907.220 kb on + strand, within PS417_17905 at 3907.266 kb on + strand, within PS417_17905 at 3907.851 kb on + strand, within PS417_17910 at 3907.851 kb on + strand, within PS417_17910 at 3907.851 kb on + strand, within PS417_17910 at 3907.851 kb on + strand at 3907.851 kb on + strand, within PS417_17910 at 3907.851 kb on + strand, within PS417_17910 at 3907.852 kb on - strand, within PS417_17910 at 3907.852 kb on - strand, within PS417_17910 at 3907.852 kb on - strand, within PS417_17910 at 3907.852 kb on - strand, within PS417_17910 at 3908.086 kb on - strand, within PS417_17910 at 3908.160 kb on + strand, within PS417_17910 at 3908.160 kb on + strand, within PS417_17910 at 3908.161 kb on - strand, within PS417_17910 at 3908.407 kb on - strand, within PS417_17910 at 3908.407 kb on - strand, within PS417_17910 at 3908.830 kb on + strand, within PS417_17910 at 3908.830 kb on + strand, within PS417_17910 at 3908.831 kb on - strand, within PS417_17910 at 3908.922 kb on + strand, within PS417_17910 at 3908.923 kb on - strand at 3908.923 kb on - strand, within PS417_17910 at 3908.923 kb on - strand, within PS417_17910 at 3908.928 kb on + strand, within PS417_17910 at 3908.929 kb on - strand, within PS417_17910 at 3909.000 kb on - strand, within PS417_17910 at 3909.280 kb on - strand at 3909.382 kb on + strand at 3909.477 kb on + strand at 3909.478 kb on - strand at 3909.478 kb on - strand at 3909.522 kb on + strand, within PS417_17915 at 3909.643 kb on - strand, within PS417_17915 at 3909.794 kb on - strand, within PS417_17915 at 3909.912 kb on + strand, within PS417_17915 at 3909.912 kb on + strand, within PS417_17915 at 3909.913 kb on - strand, within PS417_17915 at 3909.976 kb on + strand, within PS417_17915 at 3909.976 kb on + strand, within PS417_17915 at 3909.976 kb on + strand, within PS417_17915 at 3910.086 kb on + strand, within PS417_17915 at 3910.087 kb on - strand, within PS417_17915 at 3910.129 kb on + strand, within PS417_17915 at 3910.130 kb on - strand, within PS417_17915 at 3910.158 kb on + strand, within PS417_17915 at 3910.158 kb on + strand, within PS417_17915 at 3910.159 kb on - strand, within PS417_17915 at 3910.161 kb on - strand, within PS417_17915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,906,901 + +1.6 3,906,901 + -0.2 3,907,013 + -1.3 3,907,049 + +1.8 3,907,049 + +0.3 3,907,056 - -0.3 3,907,116 - +0.6 3,907,220 + PS417_17905 0.14 +0.8 3,907,266 + PS417_17905 0.22 -0.1 3,907,851 + PS417_17910 0.12 -3.0 3,907,851 + PS417_17910 0.12 -2.4 3,907,851 + PS417_17910 0.12 -0.7 3,907,851 + -1.2 3,907,851 + PS417_17910 0.12 -0.9 3,907,851 + PS417_17910 0.12 +0.5 3,907,852 - PS417_17910 0.12 -0.7 3,907,852 - PS417_17910 0.12 -0.4 3,907,852 - PS417_17910 0.12 +1.7 3,907,852 - PS417_17910 0.12 +0.5 3,908,086 - PS417_17910 0.28 +1.1 3,908,160 + PS417_17910 0.33 +0.2 3,908,160 + PS417_17910 0.33 -2.5 3,908,161 - PS417_17910 0.33 -0.0 3,908,407 - PS417_17910 0.49 -2.2 3,908,407 - PS417_17910 0.49 -0.5 3,908,830 + PS417_17910 0.77 -1.6 3,908,830 + PS417_17910 0.77 -3.2 3,908,831 - PS417_17910 0.77 +0.2 3,908,922 + PS417_17910 0.83 -0.7 3,908,923 - -1.3 3,908,923 - PS417_17910 0.84 -0.3 3,908,923 - PS417_17910 0.84 -0.0 3,908,928 + PS417_17910 0.84 -0.0 3,908,929 - PS417_17910 0.84 -0.9 3,909,000 - PS417_17910 0.89 +0.8 3,909,280 - +0.2 3,909,382 + +0.3 3,909,477 + +0.5 3,909,478 - +0.9 3,909,478 - -1.5 3,909,522 + PS417_17915 0.10 +0.8 3,909,643 - PS417_17915 0.19 +0.8 3,909,794 - PS417_17915 0.31 -2.8 3,909,912 + PS417_17915 0.40 +0.1 3,909,912 + PS417_17915 0.40 +2.3 3,909,913 - PS417_17915 0.40 -0.0 3,909,976 + PS417_17915 0.45 -1.4 3,909,976 + PS417_17915 0.45 +0.6 3,909,976 + PS417_17915 0.45 -3.1 3,910,086 + PS417_17915 0.53 -0.7 3,910,087 - PS417_17915 0.53 -2.5 3,910,129 + PS417_17915 0.56 -1.2 3,910,130 - PS417_17915 0.56 -1.2 3,910,158 + PS417_17915 0.58 +0.1 3,910,158 + PS417_17915 0.58 +0.7 3,910,159 - PS417_17915 0.58 -1.5 3,910,161 - PS417_17915 0.59 -2.3
Or see this region's nucleotide sequence