Strain Fitness in Pseudomonas simiae WCS417 around PS417_17825

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_17820 and PS417_17825 are separated by 206 nucleotidesPS417_17825 and PS417_17830 are separated by 128 nucleotidesPS417_17830 and PS417_17835 overlap by 4 nucleotides PS417_17820: PS417_17820 - hypothetical protein, at 3,890,125 to 3,892,677 _17820 PS417_17825: PS417_17825 - AraC family transcriptional regulator, at 3,892,884 to 3,893,744 _17825 PS417_17830: PS417_17830 - 4-hydroxyphenylacetate isomerase, at 3,893,873 to 3,894,538 _17830 PS417_17835: PS417_17835 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 3,894,535 to 3,895,305 _17835 Position (kb) 3892 3893 3894Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3892.028 kb on + strand, within PS417_17820at 3892.095 kb on + strand, within PS417_17820at 3892.337 kb on - strand, within PS417_17820at 3892.457 kb on + strandat 3892.457 kb on + strandat 3892.745 kb on - strandat 3892.809 kb on - strandat 3893.141 kb on - strand, within PS417_17825at 3893.548 kb on + strand, within PS417_17825at 3893.549 kb on - strand, within PS417_17825at 3893.566 kb on + strand, within PS417_17825at 3893.593 kb on + strand, within PS417_17825at 3893.594 kb on - strand, within PS417_17825at 3893.594 kb on - strand, within PS417_17825at 3893.674 kb on + strandat 3893.674 kb on + strandat 3893.696 kb on - strandat 3893.696 kb on - strandat 3893.696 kb on - strandat 3893.707 kb on + strandat 3893.822 kb on - strandat 3893.842 kb on - strandat 3893.930 kb on + strandat 3893.930 kb on + strandat 3894.011 kb on - strand, within PS417_17830at 3894.161 kb on + strand, within PS417_17830at 3894.162 kb on - strand, within PS417_17830at 3894.200 kb on + strand, within PS417_17830at 3894.201 kb on - strand, within PS417_17830at 3894.201 kb on - strand, within PS417_17830at 3894.515 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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3,892,028 + PS417_17820 0.75 +1.2
3,892,095 + PS417_17820 0.77 +0.3
3,892,337 - PS417_17820 0.87 -2.4
3,892,457 + -0.8
3,892,457 + -0.7
3,892,745 - -1.3
3,892,809 - +0.3
3,893,141 - PS417_17825 0.30 -0.1
3,893,548 + PS417_17825 0.77 +1.3
3,893,549 - PS417_17825 0.77 -0.2
3,893,566 + PS417_17825 0.79 +1.0
3,893,593 + PS417_17825 0.82 +1.3
3,893,594 - PS417_17825 0.82 +3.0
3,893,594 - PS417_17825 0.82 +0.2
3,893,674 + -1.7
3,893,674 + +1.5
3,893,696 - +0.9
3,893,696 - +0.8
3,893,696 - +0.1
3,893,707 + -0.1
3,893,822 - +0.5
3,893,842 - -0.8
3,893,930 + +1.0
3,893,930 + +0.8
3,894,011 - PS417_17830 0.21 +1.6
3,894,161 + PS417_17830 0.43 -1.9
3,894,162 - PS417_17830 0.43 +1.8
3,894,200 + PS417_17830 0.49 +0.1
3,894,201 - PS417_17830 0.49 -2.6
3,894,201 - PS417_17830 0.49 -3.3
3,894,515 - +0.3

Or see this region's nucleotide sequence