Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01735 and PS417_01745 are separated by 981 nucleotides PS417_01745 and PS417_01750 are separated by 38 nucleotides
PS417_01735: PS417_01735 - ABC transporter permease, at 385,105 to 385,956
_01735
PS417_01745: PS417_01745 - histidine ammonia-lyase, at 386,938 to 388,446
_01745
PS417_01750: PS417_01750 - histidine ammonia-lyase, at 388,485 to 390,017
_01750
Position (kb) 386
387
388
389 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 385.958 kb on + strand at 385.958 kb on + strand at 386.356 kb on + strand at 386.440 kb on - strand at 386.579 kb on - strand at 386.736 kb on + strand at 386.736 kb on + strand at 386.737 kb on - strand at 386.737 kb on - strand at 386.737 kb on - strand at 386.922 kb on + strand at 386.922 kb on + strand at 386.923 kb on - strand at 386.923 kb on - strand at 386.931 kb on + strand at 386.931 kb on + strand at 386.931 kb on + strand at 386.931 kb on + strand at 386.993 kb on + strand at 387.106 kb on + strand, within PS417_01745 at 387.269 kb on - strand, within PS417_01745 at 387.269 kb on - strand, within PS417_01745 at 387.269 kb on - strand at 387.269 kb on - strand, within PS417_01745 at 387.374 kb on - strand, within PS417_01745 at 387.385 kb on - strand, within PS417_01745 at 387.431 kb on - strand, within PS417_01745 at 387.821 kb on + strand, within PS417_01745 at 387.821 kb on + strand, within PS417_01745 at 387.822 kb on - strand, within PS417_01745 at 388.096 kb on + strand, within PS417_01745 at 388.096 kb on + strand, within PS417_01745 at 388.097 kb on - strand, within PS417_01745 at 388.363 kb on + strand at 388.364 kb on - strand at 388.478 kb on + strand at 388.479 kb on - strand at 388.538 kb on + strand at 388.539 kb on - strand at 388.542 kb on + strand at 388.879 kb on - strand, within PS417_01750 at 388.977 kb on + strand, within PS417_01750 at 388.978 kb on - strand, within PS417_01750 at 389.204 kb on + strand, within PS417_01750 at 389.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 385,958 + -1.7 385,958 + +2.8 386,356 + -0.3 386,440 - +0.8 386,579 - -0.1 386,736 + -1.2 386,736 + -1.3 386,737 - +3.4 386,737 - +1.7 386,737 - -0.8 386,922 + -0.3 386,922 + +1.6 386,923 - -0.5 386,923 - +0.0 386,931 + +0.2 386,931 + +1.4 386,931 + -1.4 386,931 + -0.5 386,993 + -2.3 387,106 + PS417_01745 0.11 -1.4 387,269 - PS417_01745 0.22 +3.0 387,269 - PS417_01745 0.22 -0.1 387,269 - -0.2 387,269 - PS417_01745 0.22 +0.7 387,374 - PS417_01745 0.29 -0.1 387,385 - PS417_01745 0.30 +0.2 387,431 - PS417_01745 0.33 +1.6 387,821 + PS417_01745 0.59 -1.7 387,821 + PS417_01745 0.59 +0.3 387,822 - PS417_01745 0.59 +0.2 388,096 + PS417_01745 0.77 +0.1 388,096 + PS417_01745 0.77 +1.3 388,097 - PS417_01745 0.77 -2.0 388,363 + +2.8 388,364 - -0.6 388,478 + -2.9 388,479 - +0.0 388,538 + +0.3 388,539 - -1.4 388,542 + -0.4 388,879 - PS417_01750 0.26 -0.5 388,977 + PS417_01750 0.32 +0.3 388,978 - PS417_01750 0.32 -0.4 389,204 + PS417_01750 0.47 -0.1 389,393 - -1.2
Or see this region's nucleotide sequence