Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01725 and PS417_01730 are separated by 69 nucleotides PS417_01730 and PS417_01735 are separated by 71 nucleotides
PS417_01725: PS417_01725 - sulfonate ABC transporter substrate-binding protein, at 382,541 to 383,998
_01725
PS417_01730: PS417_01730 - histidine ABC transporter substrate-binding protein, at 384,068 to 385,033
_01730
PS417_01735: PS417_01735 - ABC transporter permease, at 385,105 to 385,956
_01735
Position (kb) 384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 383.223 kb on - strand, within PS417_01725 at 383.363 kb on + strand, within PS417_01725 at 383.364 kb on - strand, within PS417_01725 at 383.364 kb on - strand, within PS417_01725 at 383.408 kb on + strand, within PS417_01725 at 383.409 kb on - strand, within PS417_01725 at 383.456 kb on + strand, within PS417_01725 at 383.672 kb on + strand, within PS417_01725 at 383.672 kb on + strand, within PS417_01725 at 383.766 kb on - strand at 383.813 kb on + strand, within PS417_01725 at 383.851 kb on + strand, within PS417_01725 at 383.891 kb on + strand at 383.891 kb on + strand at 383.892 kb on - strand at 383.894 kb on + strand at 383.894 kb on + strand at 383.895 kb on - strand at 383.895 kb on - strand at 383.965 kb on - strand at 384.037 kb on - strand at 384.079 kb on + strand at 384.080 kb on - strand at 384.080 kb on - strand at 384.243 kb on - strand, within PS417_01730 at 384.310 kb on + strand, within PS417_01730 at 384.425 kb on + strand, within PS417_01730 at 384.425 kb on + strand, within PS417_01730 at 384.425 kb on + strand, within PS417_01730 at 384.437 kb on + strand, within PS417_01730 at 384.438 kb on - strand, within PS417_01730 at 384.497 kb on - strand, within PS417_01730 at 384.515 kb on + strand, within PS417_01730 at 384.516 kb on - strand, within PS417_01730 at 384.516 kb on - strand, within PS417_01730 at 384.563 kb on + strand, within PS417_01730 at 384.563 kb on + strand, within PS417_01730 at 384.639 kb on - strand at 384.639 kb on - strand, within PS417_01730 at 384.695 kb on + strand, within PS417_01730 at 384.695 kb on + strand, within PS417_01730 at 384.695 kb on + strand, within PS417_01730 at 384.725 kb on + strand, within PS417_01730 at 384.726 kb on - strand, within PS417_01730 at 385.032 kb on - strand at 385.032 kb on - strand at 385.104 kb on + strand at 385.177 kb on + strand at 385.177 kb on + strand at 385.178 kb on - strand at 385.178 kb on - strand at 385.352 kb on + strand, within PS417_01735 at 385.352 kb on + strand, within PS417_01735 at 385.352 kb on + strand, within PS417_01735 at 385.528 kb on + strand, within PS417_01735 at 385.594 kb on + strand, within PS417_01735 at 385.925 kb on - strand at 385.958 kb on + strand at 385.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 383,223 - PS417_01725 0.47 +1.4 383,363 + PS417_01725 0.56 +0.3 383,364 - PS417_01725 0.56 -0.6 383,364 - PS417_01725 0.56 -2.0 383,408 + PS417_01725 0.59 +0.6 383,409 - PS417_01725 0.60 -0.1 383,456 + PS417_01725 0.63 +0.2 383,672 + PS417_01725 0.78 +0.8 383,672 + PS417_01725 0.78 -2.4 383,766 - -1.7 383,813 + PS417_01725 0.87 -1.9 383,851 + PS417_01725 0.90 -0.5 383,891 + -0.8 383,891 + +0.3 383,892 - +1.4 383,894 + +1.6 383,894 + +1.0 383,895 - -0.4 383,895 - -3.2 383,965 - +0.6 384,037 - +1.1 384,079 + -0.9 384,080 - -0.4 384,080 - +0.8 384,243 - PS417_01730 0.18 -1.1 384,310 + PS417_01730 0.25 -1.8 384,425 + PS417_01730 0.37 +2.2 384,425 + PS417_01730 0.37 +0.1 384,425 + PS417_01730 0.37 -1.4 384,437 + PS417_01730 0.38 -1.1 384,438 - PS417_01730 0.38 -0.3 384,497 - PS417_01730 0.44 -1.5 384,515 + PS417_01730 0.46 -0.7 384,516 - PS417_01730 0.46 -2.1 384,516 - PS417_01730 0.46 -0.3 384,563 + PS417_01730 0.51 +0.9 384,563 + PS417_01730 0.51 +0.2 384,639 - -0.2 384,639 - PS417_01730 0.59 -2.2 384,695 + PS417_01730 0.65 +0.5 384,695 + PS417_01730 0.65 -2.5 384,695 + PS417_01730 0.65 -1.2 384,725 + PS417_01730 0.68 -0.1 384,726 - PS417_01730 0.68 -2.3 385,032 - -0.2 385,032 - -2.1 385,104 + +1.4 385,177 + -0.4 385,177 + -0.2 385,178 - -0.5 385,178 - +0.8 385,352 + PS417_01735 0.29 -1.3 385,352 + PS417_01735 0.29 -0.2 385,352 + PS417_01735 0.29 +0.8 385,528 + PS417_01735 0.50 -0.2 385,594 + PS417_01735 0.57 +0.7 385,925 - +0.1 385,958 + -1.7 385,958 + +2.8
Or see this region's nucleotide sequence