Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17300 and PS417_17305 are separated by 596 nucleotides PS417_17305 and PS417_17310 are separated by 141 nucleotides PS417_17310 and PS417_17315 are separated by 158 nucleotides
PS417_17300: PS417_17300 - RND transporter, at 3,778,135 to 3,779,553
_17300
PS417_17305: PS417_17305 - hypothetical protein, at 3,780,150 to 3,780,359
_17305
PS417_17310: PS417_17310 - allantoate amidohydrolase, at 3,780,501 to 3,781,784
_17310
PS417_17315: PS417_17315 - nitrate reductase, at 3,781,943 to 3,783,430
_17315
Position (kb)
3780
3781
3782 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3779.507 kb on + strand at 3779.551 kb on + strand at 3779.551 kb on + strand at 3779.552 kb on - strand at 3779.708 kb on - strand at 3779.725 kb on + strand at 3779.747 kb on + strand at 3779.748 kb on - strand at 3779.748 kb on - strand at 3779.880 kb on + strand at 3779.881 kb on - strand at 3779.881 kb on - strand at 3779.914 kb on + strand at 3779.914 kb on + strand at 3779.914 kb on + strand at 3779.914 kb on + strand at 3779.915 kb on - strand at 3779.915 kb on - strand at 3779.915 kb on - strand at 3779.968 kb on + strand at 3779.968 kb on + strand at 3779.969 kb on - strand at 3779.969 kb on - strand at 3780.004 kb on - strand at 3780.058 kb on + strand at 3780.059 kb on - strand at 3780.059 kb on - strand at 3780.059 kb on - strand at 3780.069 kb on + strand at 3780.070 kb on - strand at 3780.070 kb on - strand at 3780.134 kb on + strand at 3780.135 kb on - strand at 3780.329 kb on - strand, within PS417_17305 at 3780.476 kb on - strand at 3780.483 kb on + strand at 3780.773 kb on - strand, within PS417_17310 at 3781.202 kb on - strand, within PS417_17310 at 3781.215 kb on - strand, within PS417_17310 at 3781.417 kb on - strand, within PS417_17310 at 3781.569 kb on - strand, within PS417_17310 at 3781.620 kb on + strand, within PS417_17310 at 3781.620 kb on + strand, within PS417_17310 at 3781.801 kb on + strand at 3781.883 kb on + strand at 3781.944 kb on + strand at 3782.001 kb on + strand at 3782.040 kb on + strand at 3782.041 kb on - strand at 3782.041 kb on - strand at 3782.046 kb on + strand at 3782.046 kb on + strand at 3782.046 kb on + strand at 3782.047 kb on - strand at 3782.154 kb on + strand, within PS417_17315 at 3782.262 kb on + strand, within PS417_17315 at 3782.671 kb on + strand at 3782.671 kb on + strand, within PS417_17315 at 3782.672 kb on - strand, within PS417_17315 at 3782.686 kb on + strand, within PS417_17315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,779,507 + -0.1 3,779,551 + -0.3 3,779,551 + +1.4 3,779,552 - -1.6 3,779,708 - +0.7 3,779,725 + +3.6 3,779,747 + -0.1 3,779,748 - -0.2 3,779,748 - -0.9 3,779,880 + -1.6 3,779,881 - -1.0 3,779,881 - +0.5 3,779,914 + +0.3 3,779,914 + +1.1 3,779,914 + +2.1 3,779,914 + -1.7 3,779,915 - +0.7 3,779,915 - -1.1 3,779,915 - -1.2 3,779,968 + +0.2 3,779,968 + +1.8 3,779,969 - -0.1 3,779,969 - -2.2 3,780,004 - +0.8 3,780,058 + -1.1 3,780,059 - -0.7 3,780,059 - -0.2 3,780,059 - +0.3 3,780,069 + +1.2 3,780,070 - +1.9 3,780,070 - -1.6 3,780,134 + -2.6 3,780,135 - -0.3 3,780,329 - PS417_17305 0.85 -0.1 3,780,476 - -1.6 3,780,483 + -0.3 3,780,773 - PS417_17310 0.21 +0.5 3,781,202 - PS417_17310 0.55 -0.4 3,781,215 - PS417_17310 0.56 -0.7 3,781,417 - PS417_17310 0.71 -1.6 3,781,569 - PS417_17310 0.83 -0.4 3,781,620 + PS417_17310 0.87 -0.2 3,781,620 + PS417_17310 0.87 -0.2 3,781,801 + -0.7 3,781,883 + +0.1 3,781,944 + -1.8 3,782,001 + -0.2 3,782,040 + -0.8 3,782,041 - -1.1 3,782,041 - -2.6 3,782,046 + -0.4 3,782,046 + -0.6 3,782,046 + -2.1 3,782,047 - +2.7 3,782,154 + PS417_17315 0.14 +1.2 3,782,262 + PS417_17315 0.21 -0.1 3,782,671 + +0.9 3,782,671 + PS417_17315 0.49 +0.8 3,782,672 - PS417_17315 0.49 +2.5 3,782,686 + PS417_17315 0.50 -0.7
Or see this region's nucleotide sequence