Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17195 and PS417_17200 are separated by 1 nucleotides PS417_17200 and PS417_17205 are separated by 89 nucleotides PS417_17205 and PS417_17210 are separated by 119 nucleotides
PS417_17195: PS417_17195 - pyruvate dehydrogenase, at 3,753,905 to 3,756,565
_17195
PS417_17200: PS417_17200 - hypothetical protein, at 3,756,567 to 3,757,340
_17200
PS417_17205: PS417_17205 - LysR family transcriptional regulator, at 3,757,430 to 3,758,329
_17205
PS417_17210: PS417_17210 - MFS transporter, at 3,758,449 to 3,759,654
_17210
Position (kb)
3757
3758
3759 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3756.563 kb on + strand at 3756.564 kb on - strand at 3756.569 kb on - strand at 3756.794 kb on + strand, within PS417_17200 at 3756.795 kb on - strand at 3756.795 kb on - strand, within PS417_17200 at 3756.889 kb on - strand, within PS417_17200 at 3757.053 kb on + strand, within PS417_17200 at 3757.053 kb on + strand, within PS417_17200 at 3757.053 kb on + strand at 3757.053 kb on + strand, within PS417_17200 at 3757.148 kb on - strand, within PS417_17200 at 3757.223 kb on + strand, within PS417_17200 at 3757.252 kb on + strand, within PS417_17200 at 3757.253 kb on - strand, within PS417_17200 at 3757.431 kb on + strand at 3757.468 kb on + strand at 3757.468 kb on + strand at 3757.468 kb on + strand at 3757.469 kb on - strand at 3757.469 kb on - strand at 3757.552 kb on - strand, within PS417_17205 at 3757.552 kb on - strand, within PS417_17205 at 3757.603 kb on + strand, within PS417_17205 at 3757.604 kb on - strand, within PS417_17205 at 3757.604 kb on - strand, within PS417_17205 at 3757.604 kb on - strand, within PS417_17205 at 3757.605 kb on + strand, within PS417_17205 at 3757.605 kb on + strand, within PS417_17205 at 3757.605 kb on + strand, within PS417_17205 at 3757.605 kb on + strand, within PS417_17205 at 3757.606 kb on - strand, within PS417_17205 at 3757.606 kb on - strand, within PS417_17205 at 3757.834 kb on + strand, within PS417_17205 at 3758.424 kb on + strand at 3758.511 kb on - strand at 3758.601 kb on - strand, within PS417_17210 at 3758.613 kb on - strand, within PS417_17210 at 3758.636 kb on - strand, within PS417_17210 at 3758.636 kb on - strand, within PS417_17210 at 3758.715 kb on - strand, within PS417_17210 at 3758.927 kb on + strand, within PS417_17210 at 3759.005 kb on + strand, within PS417_17210 at 3759.006 kb on - strand, within PS417_17210 at 3759.123 kb on + strand, within PS417_17210 at 3759.124 kb on - strand, within PS417_17210 at 3759.127 kb on + strand, within PS417_17210 at 3759.127 kb on + strand, within PS417_17210 at 3759.127 kb on + strand, within PS417_17210 at 3759.127 kb on + strand, within PS417_17210 at 3759.127 kb on + strand, within PS417_17210 at 3759.128 kb on - strand, within PS417_17210 at 3759.128 kb on - strand, within PS417_17210 at 3759.128 kb on - strand, within PS417_17210 at 3759.128 kb on - strand, within PS417_17210 at 3759.131 kb on + strand, within PS417_17210 at 3759.131 kb on + strand, within PS417_17210 at 3759.132 kb on - strand, within PS417_17210 at 3759.132 kb on - strand, within PS417_17210 at 3759.132 kb on - strand, within PS417_17210 at 3759.152 kb on - strand, within PS417_17210 at 3759.157 kb on + strand, within PS417_17210 at 3759.157 kb on + strand, within PS417_17210 at 3759.158 kb on - strand, within PS417_17210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,756,563 + +2.3 3,756,564 - -0.1 3,756,569 - +1.9 3,756,794 + PS417_17200 0.29 +2.2 3,756,795 - +3.5 3,756,795 - PS417_17200 0.29 +0.9 3,756,889 - PS417_17200 0.42 -1.8 3,757,053 + PS417_17200 0.63 +0.9 3,757,053 + PS417_17200 0.63 +1.8 3,757,053 + -0.7 3,757,053 + PS417_17200 0.63 -1.4 3,757,148 - PS417_17200 0.75 +2.6 3,757,223 + PS417_17200 0.85 +2.1 3,757,252 + PS417_17200 0.89 -0.1 3,757,253 - PS417_17200 0.89 +2.6 3,757,431 + +2.6 3,757,468 + +0.3 3,757,468 + +1.1 3,757,468 + -1.8 3,757,469 - -3.4 3,757,469 - -2.8 3,757,552 - PS417_17205 0.14 -0.1 3,757,552 - PS417_17205 0.14 -1.6 3,757,603 + PS417_17205 0.19 +0.6 3,757,604 - PS417_17205 0.19 -2.8 3,757,604 - PS417_17205 0.19 -1.6 3,757,604 - PS417_17205 0.19 -4.9 3,757,605 + PS417_17205 0.19 -1.0 3,757,605 + PS417_17205 0.19 +1.3 3,757,605 + PS417_17205 0.19 -0.5 3,757,605 + PS417_17205 0.19 -0.1 3,757,606 - PS417_17205 0.20 -1.4 3,757,606 - PS417_17205 0.20 -2.9 3,757,834 + PS417_17205 0.45 -1.1 3,758,424 + +0.9 3,758,511 - -1.7 3,758,601 - PS417_17210 0.13 -2.0 3,758,613 - PS417_17210 0.14 -2.5 3,758,636 - PS417_17210 0.16 -3.0 3,758,636 - PS417_17210 0.16 -2.8 3,758,715 - PS417_17210 0.22 -1.8 3,758,927 + PS417_17210 0.40 +0.5 3,759,005 + PS417_17210 0.46 +0.3 3,759,006 - PS417_17210 0.46 -1.7 3,759,123 + PS417_17210 0.56 -2.4 3,759,124 - PS417_17210 0.56 +0.7 3,759,127 + PS417_17210 0.56 -1.1 3,759,127 + PS417_17210 0.56 -1.7 3,759,127 + PS417_17210 0.56 -0.2 3,759,127 + PS417_17210 0.56 -1.6 3,759,127 + PS417_17210 0.56 +0.3 3,759,128 - PS417_17210 0.56 +2.9 3,759,128 - PS417_17210 0.56 -1.1 3,759,128 - PS417_17210 0.56 +2.5 3,759,128 - PS417_17210 0.56 -0.1 3,759,131 + PS417_17210 0.57 +0.0 3,759,131 + PS417_17210 0.57 -3.3 3,759,132 - PS417_17210 0.57 -1.8 3,759,132 - PS417_17210 0.57 -1.1 3,759,132 - PS417_17210 0.57 -2.0 3,759,152 - PS417_17210 0.58 +0.6 3,759,157 + PS417_17210 0.59 -3.0 3,759,157 + PS417_17210 0.59 +1.6 3,759,158 - PS417_17210 0.59 +0.5
Or see this region's nucleotide sequence