Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16860 and PS417_16865 are separated by 101 nucleotides PS417_16865 and PS417_16870 overlap by 4 nucleotides PS417_16870 and PS417_16875 are separated by 192 nucleotides PS417_16875 and PS417_16880 are separated by 112 nucleotides
PS417_16860: PS417_16860 - diadenosine tetraphosphate hydrolase, at 3,676,780 to 3,677,238
_16860
PS417_16865: PS417_16865 - HxlR family transcriptional regulator, at 3,677,340 to 3,677,669
_16865
PS417_16870: PS417_16870 - short-chain dehydrogenase, at 3,677,666 to 3,678,448
_16870
PS417_16875: PS417_16875 - signal peptidase, at 3,678,641 to 3,679,309
_16875
PS417_16880: PS417_16880 - deaminase/reductase, at 3,679,422 to 3,679,985
_16880
Position (kb)
3677
3678
3679 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3676.723 kb on + strand at 3676.724 kb on - strand at 3676.778 kb on + strand at 3676.842 kb on - strand at 3676.857 kb on + strand, within PS417_16860 at 3676.857 kb on + strand, within PS417_16860 at 3676.860 kb on - strand, within PS417_16860 at 3676.860 kb on - strand, within PS417_16860 at 3676.915 kb on - strand, within PS417_16860 at 3677.094 kb on - strand, within PS417_16860 at 3677.167 kb on - strand, within PS417_16860 at 3677.212 kb on - strand at 3677.274 kb on - strand at 3677.274 kb on - strand at 3677.274 kb on - strand at 3677.301 kb on + strand at 3677.335 kb on - strand at 3677.353 kb on - strand at 3677.586 kb on + strand, within PS417_16865 at 3677.700 kb on + strand at 3677.994 kb on + strand, within PS417_16870 at 3678.197 kb on + strand, within PS417_16870 at 3678.428 kb on + strand at 3678.455 kb on + strand at 3678.542 kb on - strand at 3678.606 kb on - strand at 3678.966 kb on + strand, within PS417_16875 at 3678.966 kb on + strand, within PS417_16875 at 3678.967 kb on - strand, within PS417_16875 at 3678.967 kb on - strand, within PS417_16875 at 3678.967 kb on - strand, within PS417_16875 at 3678.970 kb on + strand, within PS417_16875 at 3679.309 kb on + strand at 3679.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,676,723 + +1.2 3,676,724 - -0.9 3,676,778 + +0.4 3,676,842 - -1.1 3,676,857 + PS417_16860 0.17 +0.1 3,676,857 + PS417_16860 0.17 +1.4 3,676,860 - PS417_16860 0.17 -1.0 3,676,860 - PS417_16860 0.17 -0.7 3,676,915 - PS417_16860 0.29 -0.1 3,677,094 - PS417_16860 0.68 -0.3 3,677,167 - PS417_16860 0.84 +0.1 3,677,212 - -0.1 3,677,274 - +1.6 3,677,274 - -0.2 3,677,274 - +0.0 3,677,301 + -0.2 3,677,335 - -0.7 3,677,353 - +0.4 3,677,586 + PS417_16865 0.75 -2.7 3,677,700 + +1.1 3,677,994 + PS417_16870 0.42 -3.4 3,678,197 + PS417_16870 0.68 -1.0 3,678,428 + +0.7 3,678,455 + +0.1 3,678,542 - -2.2 3,678,606 - -1.7 3,678,966 + PS417_16875 0.49 +0.2 3,678,966 + PS417_16875 0.49 -2.8 3,678,967 - PS417_16875 0.49 +1.1 3,678,967 - PS417_16875 0.49 -0.6 3,678,967 - PS417_16875 0.49 +1.8 3,678,970 + PS417_16875 0.49 -3.7 3,679,309 + -0.9 3,679,310 - -0.2
Or see this region's nucleotide sequence