Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16470 and PS417_16475 are separated by 16 nucleotides PS417_16475 and PS417_16480 overlap by 1 nucleotides PS417_16480 and PS417_16485 are separated by 10 nucleotides PS417_16485 and PS417_16490 are separated by 54 nucleotides
PS417_16470: PS417_16470 - seryl-tRNA synthetase, at 3,597,703 to 3,598,983
_16470
PS417_16475: PS417_16475 - camphor resistance protein CrcB, at 3,599,000 to 3,599,374
_16475
PS417_16480: PS417_16480 - recombination factor protein RarA, at 3,599,374 to 3,600,696
_16480
PS417_16485: PS417_16485 - membrane protein, at 3,600,707 to 3,601,330
_16485
PS417_16490: PS417_16490 - cell division protein FtsK, at 3,601,385 to 3,603,793
_16490
Position (kb)
3599
3600
3601 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3599.151 kb on - strand, within PS417_16475 at 3599.218 kb on - strand, within PS417_16475 at 3599.218 kb on - strand, within PS417_16475 at 3599.330 kb on - strand, within PS417_16475 at 3599.358 kb on - strand at 3599.504 kb on + strand at 3599.546 kb on + strand, within PS417_16480 at 3599.669 kb on + strand, within PS417_16480 at 3599.703 kb on - strand, within PS417_16480 at 3599.703 kb on - strand, within PS417_16480 at 3599.928 kb on + strand, within PS417_16480 at 3599.928 kb on + strand, within PS417_16480 at 3599.929 kb on - strand, within PS417_16480 at 3599.952 kb on - strand, within PS417_16480 at 3599.952 kb on - strand, within PS417_16480 at 3600.224 kb on + strand, within PS417_16480 at 3600.225 kb on - strand, within PS417_16480 at 3600.225 kb on - strand, within PS417_16480 at 3600.317 kb on + strand, within PS417_16480 at 3600.318 kb on - strand, within PS417_16480 at 3600.318 kb on - strand, within PS417_16480 at 3600.318 kb on - strand, within PS417_16480 at 3600.623 kb on + strand at 3600.624 kb on - strand at 3600.708 kb on + strand at 3601.573 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,599,151 - PS417_16475 0.40 -0.7 3,599,218 - PS417_16475 0.58 -0.0 3,599,218 - PS417_16475 0.58 -0.6 3,599,330 - PS417_16475 0.88 -0.9 3,599,358 - +0.4 3,599,504 + -1.6 3,599,546 + PS417_16480 0.13 +3.9 3,599,669 + PS417_16480 0.22 -0.5 3,599,703 - PS417_16480 0.25 +1.3 3,599,703 - PS417_16480 0.25 +1.6 3,599,928 + PS417_16480 0.42 +2.1 3,599,928 + PS417_16480 0.42 +1.0 3,599,929 - PS417_16480 0.42 +0.2 3,599,952 - PS417_16480 0.44 -0.1 3,599,952 - PS417_16480 0.44 +1.4 3,600,224 + PS417_16480 0.64 +0.9 3,600,225 - PS417_16480 0.64 -0.3 3,600,225 - PS417_16480 0.64 -0.1 3,600,317 + PS417_16480 0.71 +0.6 3,600,318 - PS417_16480 0.71 -1.4 3,600,318 - PS417_16480 0.71 +0.7 3,600,318 - PS417_16480 0.71 -0.6 3,600,623 + -1.6 3,600,624 - -0.4 3,600,708 + +0.0 3,601,573 - -1.1
Or see this region's nucleotide sequence