Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16180 and PS417_16185 are separated by 123 nucleotides PS417_16185 and PS417_16190 are separated by 86 nucleotides PS417_16190 and PS417_16195 are separated by 86 nucleotides PS417_16195 and PS417_16200 are separated by 72 nucleotides
PS417_16180: PS417_16180 - MFS transporter, at 3,547,115 to 3,548,299
_16180
PS417_16185: PS417_16185 - hypothetical protein, at 3,548,423 to 3,548,782
_16185
PS417_16190: PS417_16190 - bile acid:sodium symporter, at 3,548,869 to 3,549,867
_16190
PS417_16195: PS417_16195 - AraC family transcriptional regulator, at 3,549,954 to 3,550,742
_16195
PS417_16200: PS417_16200 - sulfonate ABC transporter substrate-binding protein, at 3,550,815 to 3,551,816
_16200
Position (kb)
3548
3549
3550 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3548.036 kb on - strand, within PS417_16180 at 3548.130 kb on + strand, within PS417_16180 at 3548.131 kb on - strand, within PS417_16180 at 3548.367 kb on - strand at 3548.367 kb on - strand at 3548.368 kb on + strand at 3548.449 kb on - strand at 3548.449 kb on - strand at 3548.490 kb on - strand, within PS417_16185 at 3548.494 kb on - strand, within PS417_16185 at 3548.506 kb on - strand, within PS417_16185 at 3548.509 kb on - strand, within PS417_16185 at 3548.834 kb on + strand at 3548.865 kb on + strand at 3549.236 kb on + strand, within PS417_16190 at 3549.403 kb on + strand, within PS417_16190 at 3549.403 kb on + strand, within PS417_16190 at 3549.404 kb on - strand, within PS417_16190 at 3549.555 kb on - strand, within PS417_16190 at 3549.555 kb on - strand, within PS417_16190 at 3549.569 kb on + strand, within PS417_16190 at 3549.570 kb on - strand, within PS417_16190 at 3549.570 kb on - strand, within PS417_16190 at 3549.570 kb on - strand, within PS417_16190 at 3549.570 kb on - strand, within PS417_16190 at 3549.867 kb on + strand at 3549.868 kb on - strand at 3549.916 kb on + strand at 3550.234 kb on - strand at 3550.341 kb on + strand, within PS417_16195 at 3550.777 kb on + strand at 3550.778 kb on - strand at 3550.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,548,036 - PS417_16180 0.78 -2.1 3,548,130 + PS417_16180 0.86 -0.2 3,548,131 - PS417_16180 0.86 -0.2 3,548,367 - -2.2 3,548,367 - +0.4 3,548,368 + +1.6 3,548,449 - -1.9 3,548,449 - -0.3 3,548,490 - PS417_16185 0.19 -0.8 3,548,494 - PS417_16185 0.20 +0.1 3,548,506 - PS417_16185 0.23 +0.4 3,548,509 - PS417_16185 0.24 +0.3 3,548,834 + -0.1 3,548,865 + -0.6 3,549,236 + PS417_16190 0.37 -0.5 3,549,403 + PS417_16190 0.53 +1.5 3,549,403 + PS417_16190 0.53 -0.3 3,549,404 - PS417_16190 0.54 +0.5 3,549,555 - PS417_16190 0.69 +0.6 3,549,555 - PS417_16190 0.69 -1.3 3,549,569 + PS417_16190 0.70 +0.7 3,549,570 - PS417_16190 0.70 +0.6 3,549,570 - PS417_16190 0.70 -1.5 3,549,570 - PS417_16190 0.70 -1.3 3,549,570 - PS417_16190 0.70 -1.5 3,549,867 + -1.5 3,549,868 - +0.9 3,549,916 + -1.6 3,550,234 - -0.5 3,550,341 + PS417_16195 0.49 +2.2 3,550,777 + -1.6 3,550,778 - -0.2 3,550,853 - +0.2
Or see this region's nucleotide sequence