Strain Fitness in Pseudomonas simiae WCS417 around PS417_01550

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01545 and PS417_01550 overlap by 4 nucleotidesPS417_01550 and PS417_01555 are separated by 325 nucleotides PS417_01545: PS417_01545 - adenine glycosylase, at 340,665 to 341,732 _01545 PS417_01550: PS417_01550 - cell envelope biogenesis protein AsmA, at 341,729 to 343,939 _01550 PS417_01555: PS417_01555 - MFS transporter, at 344,265 to 345,926 _01555 Position (kb) 341 342 343 344Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 340.760 kb on + strandat 340.760 kb on + strandat 340.762 kb on + strandat 340.763 kb on - strandat 340.763 kb on - strandat 340.763 kb on - strandat 341.485 kb on + strand, within PS417_01545at 341.485 kb on + strand, within PS417_01545at 341.486 kb on - strand, within PS417_01545at 341.486 kb on - strand, within PS417_01545at 341.514 kb on + strand, within PS417_01545at 341.514 kb on + strand, within PS417_01545at 341.581 kb on + strand, within PS417_01545at 341.582 kb on - strand, within PS417_01545at 341.693 kb on - strandat 341.986 kb on + strand, within PS417_01550at 342.225 kb on - strand, within PS417_01550at 342.225 kb on - strand, within PS417_01550at 342.462 kb on + strand, within PS417_01550at 342.462 kb on + strand, within PS417_01550at 342.463 kb on - strand, within PS417_01550at 342.763 kb on - strand, within PS417_01550at 342.763 kb on - strand, within PS417_01550at 343.076 kb on + strand, within PS417_01550at 343.411 kb on - strand, within PS417_01550at 343.608 kb on - strand, within PS417_01550at 343.613 kb on + strand, within PS417_01550at 343.834 kb on + strandat 343.834 kb on + strandat 343.835 kb on - strandat 343.835 kb on - strandat 343.835 kb on - strandat 343.837 kb on - strandat 343.837 kb on - strandat 343.837 kb on - strandat 343.837 kb on - strandat 343.874 kb on + strandat 343.875 kb on - strandat 343.907 kb on + strandat 343.908 kb on - strandat 343.953 kb on + strandat 343.954 kb on - strandat 343.954 kb on - strandat 343.982 kb on + strandat 343.982 kb on + strandat 343.983 kb on - strandat 343.983 kb on - strandat 343.983 kb on - strandat 343.984 kb on + strandat 344.020 kb on - strandat 344.027 kb on - strandat 344.089 kb on - strandat 344.122 kb on - strandat 344.122 kb on - strandat 344.167 kb on - strandat 344.242 kb on - strandat 344.242 kb on - strandat 344.242 kb on - strandat 344.245 kb on - strandat 344.406 kb on + strandat 344.547 kb on + strand, within PS417_01555at 344.547 kb on + strand, within PS417_01555at 344.547 kb on + strand, within PS417_01555at 344.547 kb on + strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.548 kb on - strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.621 kb on + strand, within PS417_01555at 344.622 kb on - strand, within PS417_01555at 344.682 kb on - strand, within PS417_01555at 344.685 kb on + strandat 344.685 kb on + strand, within PS417_01555at 344.685 kb on + strand, within PS417_01555at 344.685 kb on + strand, within PS417_01555at 344.686 kb on - strand, within PS417_01555at 344.736 kb on - strand, within PS417_01555at 344.754 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.756 kb on + strand, within PS417_01555at 344.757 kb on - strand, within PS417_01555

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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340,760 + +0.1
340,760 + -0.1
340,762 + -0.5
340,763 - +0.4
340,763 - -1.0
340,763 - +0.2
341,485 + PS417_01545 0.77 +0.1
341,485 + PS417_01545 0.77 +0.3
341,486 - PS417_01545 0.77 -1.2
341,486 - PS417_01545 0.77 -0.5
341,514 + PS417_01545 0.79 +0.9
341,514 + PS417_01545 0.79 +0.1
341,581 + PS417_01545 0.86 -1.0
341,582 - PS417_01545 0.86 -0.2
341,693 - -2.1
341,986 + PS417_01550 0.12 +1.5
342,225 - PS417_01550 0.22 +0.5
342,225 - PS417_01550 0.22 -0.2
342,462 + PS417_01550 0.33 -2.6
342,462 + PS417_01550 0.33 +1.1
342,463 - PS417_01550 0.33 +2.1
342,763 - PS417_01550 0.47 +1.4
342,763 - PS417_01550 0.47 +0.7
343,076 + PS417_01550 0.61 +0.6
343,411 - PS417_01550 0.76 -1.7
343,608 - PS417_01550 0.85 +2.3
343,613 + PS417_01550 0.85 +1.1
343,834 + -1.5
343,834 + +2.1
343,835 - -0.2
343,835 - +2.1
343,835 - -0.6
343,837 - +1.1
343,837 - +1.2
343,837 - +0.9
343,837 - -1.0
343,874 + +2.2
343,875 - +0.4
343,907 + +1.4
343,908 - -1.8
343,953 + +0.9
343,954 - +0.8
343,954 - -0.3
343,982 + +0.1
343,982 + +1.9
343,983 - -2.7
343,983 - -1.0
343,983 - -1.4
343,984 + -1.9
344,020 - -1.5
344,027 - -0.3
344,089 - -2.9
344,122 - -4.1
344,122 - -2.1
344,167 - +0.2
344,242 - -3.2
344,242 - -2.7
344,242 - -2.5
344,245 - +0.1
344,406 + +0.6
344,547 + PS417_01555 0.17 +0.4
344,547 + PS417_01555 0.17 +0.4
344,547 + PS417_01555 0.17 +0.2
344,547 + PS417_01555 0.17 -0.2
344,548 - PS417_01555 0.17 -1.0
344,548 - PS417_01555 0.17 +2.8
344,548 - PS417_01555 0.17 -3.9
344,548 - PS417_01555 0.17 -1.0
344,548 - PS417_01555 0.17 -1.0
344,548 - PS417_01555 0.17 +0.1
344,621 + PS417_01555 0.21 -1.4
344,621 + PS417_01555 0.21 +0.4
344,621 + PS417_01555 0.21 +2.4
344,621 + PS417_01555 0.21 +2.5
344,622 - PS417_01555 0.21 -0.5
344,682 - PS417_01555 0.25 -5.3
344,685 + +0.8
344,685 + PS417_01555 0.25 +0.4
344,685 + PS417_01555 0.25 +0.2
344,685 + PS417_01555 0.25 -1.4
344,686 - PS417_01555 0.25 +0.5
344,736 - PS417_01555 0.28 -1.1
344,754 + PS417_01555 0.29 +0.4
344,756 + PS417_01555 0.30 +0.5
344,756 + PS417_01555 0.30 -1.0
344,756 + PS417_01555 0.30 +1.1
344,756 + PS417_01555 0.30 +1.2
344,757 - PS417_01555 0.30 +0.3

Or see this region's nucleotide sequence