Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15210 and PS417_15215 are separated by 132 nucleotides PS417_15215 and PS417_15220 are separated by 132 nucleotides PS417_15220 and PS417_15225 are separated by 294 nucleotides
PS417_15210: PS417_15210 - protein activator of alkane oxidation PraB, at 3,335,521 to 3,336,015
_15210
PS417_15215: PS417_15215 - protein activator of alkane oxidation PraA, at 3,336,148 to 3,336,642
_15215
PS417_15220: PS417_15220 - alkane 1-monooxygenase, at 3,336,775 to 3,338,055
_15220
PS417_15225: PS417_15225 - alpha/beta hydrolase, at 3,338,350 to 3,339,273
_15225
Position (kb)
3336
3337
3338
3339 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3335.940 kb on - strand, within PS417_15210 at 3336.183 kb on + strand at 3336.261 kb on + strand, within PS417_15215 at 3336.436 kb on + strand, within PS417_15215 at 3336.581 kb on + strand, within PS417_15215 at 3336.648 kb on - strand at 3336.648 kb on - strand at 3336.751 kb on + strand at 3336.764 kb on + strand at 3336.800 kb on + strand at 3336.914 kb on + strand, within PS417_15220 at 3336.914 kb on + strand, within PS417_15220 at 3336.914 kb on + strand, within PS417_15220 at 3336.915 kb on - strand, within PS417_15220 at 3336.987 kb on - strand, within PS417_15220 at 3336.987 kb on - strand, within PS417_15220 at 3336.990 kb on + strand, within PS417_15220 at 3336.990 kb on + strand, within PS417_15220 at 3336.991 kb on - strand, within PS417_15220 at 3337.145 kb on + strand, within PS417_15220 at 3337.146 kb on - strand, within PS417_15220 at 3337.175 kb on + strand, within PS417_15220 at 3337.175 kb on + strand, within PS417_15220 at 3337.176 kb on - strand, within PS417_15220 at 3337.254 kb on - strand, within PS417_15220 at 3337.254 kb on - strand, within PS417_15220 at 3337.472 kb on + strand, within PS417_15220 at 3337.472 kb on + strand, within PS417_15220 at 3337.473 kb on - strand, within PS417_15220 at 3337.473 kb on - strand, within PS417_15220 at 3337.473 kb on - strand, within PS417_15220 at 3337.598 kb on + strand, within PS417_15220 at 3337.632 kb on + strand, within PS417_15220 at 3337.632 kb on + strand, within PS417_15220 at 3337.633 kb on - strand, within PS417_15220 at 3337.633 kb on - strand at 3337.633 kb on - strand, within PS417_15220 at 3337.647 kb on - strand, within PS417_15220 at 3337.647 kb on - strand, within PS417_15220 at 3337.647 kb on - strand, within PS417_15220 at 3337.809 kb on - strand, within PS417_15220 at 3337.909 kb on + strand, within PS417_15220 at 3337.909 kb on + strand, within PS417_15220 at 3337.909 kb on + strand, within PS417_15220 at 3337.910 kb on - strand, within PS417_15220 at 3337.966 kb on + strand at 3337.966 kb on + strand at 3337.967 kb on - strand at 3337.967 kb on - strand at 3338.130 kb on - strand at 3338.161 kb on + strand at 3338.161 kb on + strand at 3338.663 kb on + strand, within PS417_15225 at 3338.663 kb on + strand at 3338.664 kb on - strand, within PS417_15225 at 3338.969 kb on - strand, within PS417_15225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,335,940 - PS417_15210 0.85 +0.5 3,336,183 + +0.4 3,336,261 + PS417_15215 0.23 -0.5 3,336,436 + PS417_15215 0.58 +1.3 3,336,581 + PS417_15215 0.87 +0.4 3,336,648 - -2.6 3,336,648 - +1.0 3,336,751 + -3.5 3,336,764 + -1.0 3,336,800 + -0.1 3,336,914 + PS417_15220 0.11 -0.9 3,336,914 + PS417_15220 0.11 -0.2 3,336,914 + PS417_15220 0.11 +0.6 3,336,915 - PS417_15220 0.11 -2.8 3,336,987 - PS417_15220 0.17 -0.2 3,336,987 - PS417_15220 0.17 +1.1 3,336,990 + PS417_15220 0.17 +1.9 3,336,990 + PS417_15220 0.17 +0.3 3,336,991 - PS417_15220 0.17 -0.2 3,337,145 + PS417_15220 0.29 -0.5 3,337,146 - PS417_15220 0.29 +1.1 3,337,175 + PS417_15220 0.31 +0.7 3,337,175 + PS417_15220 0.31 -1.8 3,337,176 - PS417_15220 0.31 -2.4 3,337,254 - PS417_15220 0.37 +0.7 3,337,254 - PS417_15220 0.37 +0.5 3,337,472 + PS417_15220 0.54 +1.2 3,337,472 + PS417_15220 0.54 +0.8 3,337,473 - PS417_15220 0.54 +0.1 3,337,473 - PS417_15220 0.54 +0.8 3,337,473 - PS417_15220 0.54 +1.1 3,337,598 + PS417_15220 0.64 +0.9 3,337,632 + PS417_15220 0.67 +0.3 3,337,632 + PS417_15220 0.67 +1.5 3,337,633 - PS417_15220 0.67 +0.9 3,337,633 - -0.2 3,337,633 - PS417_15220 0.67 +0.2 3,337,647 - PS417_15220 0.68 +0.2 3,337,647 - PS417_15220 0.68 +0.9 3,337,647 - PS417_15220 0.68 +0.5 3,337,809 - PS417_15220 0.81 -0.5 3,337,909 + PS417_15220 0.89 -0.8 3,337,909 + PS417_15220 0.89 +0.6 3,337,909 + PS417_15220 0.89 +1.2 3,337,910 - PS417_15220 0.89 +0.6 3,337,966 + -1.2 3,337,966 + -0.8 3,337,967 - +2.4 3,337,967 - -1.4 3,338,130 - +0.0 3,338,161 + +0.8 3,338,161 + +0.2 3,338,663 + PS417_15225 0.34 +0.5 3,338,663 + -0.8 3,338,664 - PS417_15225 0.34 -1.2 3,338,969 - PS417_15225 0.67 -1.7
Or see this region's nucleotide sequence