Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14970 and PS417_14975 overlap by 45 nucleotides PS417_14975 and PS417_14980 are separated by 151 nucleotides
PS417_14970: PS417_14970 - MFS transporter, at 3,272,620 to 3,273,819
_14970
PS417_14975: PS417_14975 - tRNA hydroxylase, at 3,273,775 to 3,274,377
_14975
PS417_14980: PS417_14980 - universal stress protein UspA, at 3,274,529 to 3,275,392
_14980
Position (kb)
3273
3274
3275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3273.004 kb on + strand, within PS417_14970 at 3273.005 kb on - strand, within PS417_14970 at 3273.023 kb on - strand, within PS417_14970 at 3273.373 kb on + strand, within PS417_14970 at 3273.373 kb on + strand, within PS417_14970 at 3273.373 kb on + strand, within PS417_14970 at 3273.373 kb on + strand, within PS417_14970 at 3273.373 kb on + strand, within PS417_14970 at 3273.373 kb on + strand, within PS417_14970 at 3273.374 kb on - strand, within PS417_14970 at 3273.374 kb on - strand, within PS417_14970 at 3273.472 kb on + strand, within PS417_14970 at 3273.521 kb on + strand, within PS417_14970 at 3273.522 kb on - strand, within PS417_14970 at 3273.527 kb on + strand, within PS417_14970 at 3273.527 kb on + strand, within PS417_14970 at 3273.529 kb on + strand, within PS417_14970 at 3273.530 kb on - strand, within PS417_14970 at 3273.714 kb on + strand at 3273.893 kb on + strand, within PS417_14975 at 3273.908 kb on + strand, within PS417_14975 at 3274.097 kb on + strand, within PS417_14975 at 3274.223 kb on + strand, within PS417_14975 at 3274.223 kb on + strand, within PS417_14975 at 3274.416 kb on + strand at 3274.489 kb on + strand at 3274.489 kb on + strand at 3274.490 kb on - strand at 3274.879 kb on + strand, within PS417_14980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,273,004 + PS417_14970 0.32 -4.6 3,273,005 - PS417_14970 0.32 -2.5 3,273,023 - PS417_14970 0.34 -0.2 3,273,373 + PS417_14970 0.63 -1.5 3,273,373 + PS417_14970 0.63 -1.2 3,273,373 + PS417_14970 0.63 -3.5 3,273,373 + PS417_14970 0.63 -0.5 3,273,373 + PS417_14970 0.63 +0.8 3,273,373 + PS417_14970 0.63 -0.1 3,273,374 - PS417_14970 0.63 +1.8 3,273,374 - PS417_14970 0.63 -2.7 3,273,472 + PS417_14970 0.71 +0.2 3,273,521 + PS417_14970 0.75 +0.1 3,273,522 - PS417_14970 0.75 +1.1 3,273,527 + PS417_14970 0.76 +0.5 3,273,527 + PS417_14970 0.76 -3.8 3,273,529 + PS417_14970 0.76 -0.6 3,273,530 - PS417_14970 0.76 -0.2 3,273,714 + -0.3 3,273,893 + PS417_14975 0.20 -0.2 3,273,908 + PS417_14975 0.22 +0.0 3,274,097 + PS417_14975 0.53 -0.3 3,274,223 + PS417_14975 0.74 -0.9 3,274,223 + PS417_14975 0.74 -1.4 3,274,416 + -1.6 3,274,489 + +1.1 3,274,489 + -0.3 3,274,490 - +2.1 3,274,879 + PS417_14980 0.41 +0.1
Or see this region's nucleotide sequence