Strain Fitness in Pseudomonas simiae WCS417 around PS417_14945

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_14940 and PS417_14945 are separated by 116 nucleotidesPS417_14945 and PS417_14950 are separated by 5 nucleotidesPS417_14950 and PS417_14955 are separated by 152 nucleotides PS417_14940: PS417_14940 - LysR family transcriptional regulator, at 3,262,631 to 3,263,521 _14940 PS417_14945: PS417_14945 - beta-lactamase, at 3,263,638 to 3,265,452 _14945 PS417_14950: PS417_14950 - structural protein MipA, at 3,265,458 to 3,266,261 _14950 PS417_14955: PS417_14955 - hypothetical protein, at 3,266,414 to 3,270,472 _14955 Position (kb) 3263 3264 3265 3266Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2 3 4at 3263.044 kb on - strand, within PS417_14940at 3263.741 kb on - strandat 3264.020 kb on - strand, within PS417_14945at 3264.020 kb on - strand, within PS417_14945at 3264.140 kb on - strand, within PS417_14945at 3264.140 kb on - strand, within PS417_14945at 3264.211 kb on + strand, within PS417_14945at 3264.457 kb on + strandat 3264.471 kb on - strand, within PS417_14945at 3264.488 kb on - strandat 3264.636 kb on + strand, within PS417_14945at 3264.898 kb on + strand, within PS417_14945at 3265.065 kb on - strand, within PS417_14945at 3265.065 kb on - strand, within PS417_14945at 3265.196 kb on - strand, within PS417_14945at 3265.309 kb on + strandat 3265.309 kb on + strandat 3265.310 kb on - strandat 3265.310 kb on - strandat 3265.429 kb on - strandat 3265.554 kb on + strand, within PS417_14950at 3265.840 kb on - strand, within PS417_14950at 3265.957 kb on - strand, within PS417_14950at 3266.102 kb on + strand, within PS417_14950at 3266.251 kb on - strandat 3266.410 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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3,263,044 - PS417_14940 0.46 +0.2
3,263,741 - -4.0
3,264,020 - PS417_14945 0.21 -6.6
3,264,020 - PS417_14945 0.21 -0.2
3,264,140 - PS417_14945 0.28 -3.2
3,264,140 - PS417_14945 0.28 -3.5
3,264,211 + PS417_14945 0.32 -4.8
3,264,457 + -1.3
3,264,471 - PS417_14945 0.46 -2.4
3,264,488 - +0.3
3,264,636 + PS417_14945 0.55 -2.2
3,264,898 + PS417_14945 0.69 -2.2
3,265,065 - PS417_14945 0.79 -1.8
3,265,065 - PS417_14945 0.79 +4.3
3,265,196 - PS417_14945 0.86 -2.2
3,265,309 + -2.7
3,265,309 + -4.4
3,265,310 - -5.6
3,265,310 - -2.5
3,265,429 - -2.9
3,265,554 + PS417_14950 0.12 +2.4
3,265,840 - PS417_14950 0.48 +1.8
3,265,957 - PS417_14950 0.62 +1.3
3,266,102 + PS417_14950 0.80 +0.7
3,266,251 - -0.8
3,266,410 - -0.6

Or see this region's nucleotide sequence