Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14895 and PS417_14900 are separated by 132 nucleotides PS417_14900 and PS417_14905 overlap by 1 nucleotides PS417_14905 and PS417_14910 are separated by 149 nucleotides
PS417_14895: PS417_14895 - beta-lactamase/D-alanine carboxypeptidase, at 3,252,657 to 3,253,811
_14895
PS417_14900: PS417_14900 - C4-dicarboxylate ABC transporter permease, at 3,253,944 to 3,255,224
_14900
PS417_14905: PS417_14905 - C4-dicarboxylate ABC transporter, at 3,255,224 to 3,255,856
_14905
PS417_14910: PS417_14910 - C4-dicarboxylate ABC transporter, at 3,256,006 to 3,256,989
_14910
Position (kb)
3253
3254
3255
3256 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3253.057 kb on - strand, within PS417_14895 at 3253.330 kb on - strand, within PS417_14895 at 3253.387 kb on - strand, within PS417_14895 at 3253.422 kb on + strand, within PS417_14895 at 3253.423 kb on - strand, within PS417_14895 at 3253.423 kb on - strand, within PS417_14895 at 3253.438 kb on + strand, within PS417_14895 at 3253.438 kb on + strand, within PS417_14895 at 3253.438 kb on + strand, within PS417_14895 at 3253.438 kb on + strand, within PS417_14895 at 3253.439 kb on - strand, within PS417_14895 at 3253.497 kb on + strand, within PS417_14895 at 3253.497 kb on + strand, within PS417_14895 at 3253.500 kb on + strand at 3253.501 kb on - strand, within PS417_14895 at 3253.501 kb on - strand, within PS417_14895 at 3253.501 kb on - strand, within PS417_14895 at 3253.501 kb on - strand, within PS417_14895 at 3253.542 kb on + strand, within PS417_14895 at 3253.542 kb on + strand, within PS417_14895 at 3253.696 kb on - strand at 3253.696 kb on - strand at 3253.809 kb on + strand at 3253.946 kb on - strand at 3253.946 kb on - strand at 3253.946 kb on - strand at 3253.949 kb on + strand at 3253.950 kb on - strand at 3253.991 kb on - strand at 3253.991 kb on - strand at 3254.458 kb on + strand, within PS417_14900 at 3254.459 kb on - strand, within PS417_14900 at 3254.533 kb on + strand, within PS417_14900 at 3254.656 kb on + strand, within PS417_14900 at 3254.656 kb on + strand, within PS417_14900 at 3254.656 kb on + strand, within PS417_14900 at 3254.656 kb on + strand, within PS417_14900 at 3254.657 kb on - strand, within PS417_14900 at 3254.750 kb on - strand, within PS417_14900 at 3255.063 kb on - strand, within PS417_14900 at 3255.063 kb on - strand at 3255.064 kb on + strand, within PS417_14900 at 3255.064 kb on + strand, within PS417_14900 at 3255.064 kb on + strand at 3255.195 kb on + strand at 3255.483 kb on + strand, within PS417_14905 at 3255.768 kb on + strand, within PS417_14905 at 3255.880 kb on + strand at 3255.880 kb on + strand at 3256.096 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,253,057 - PS417_14895 0.35 -0.2 3,253,330 - PS417_14895 0.58 +1.0 3,253,387 - PS417_14895 0.63 +0.9 3,253,422 + PS417_14895 0.66 +0.6 3,253,423 - PS417_14895 0.66 +2.1 3,253,423 - PS417_14895 0.66 -2.4 3,253,438 + PS417_14895 0.68 +0.7 3,253,438 + PS417_14895 0.68 +0.1 3,253,438 + PS417_14895 0.68 -1.0 3,253,438 + PS417_14895 0.68 -4.0 3,253,439 - PS417_14895 0.68 +1.6 3,253,497 + PS417_14895 0.73 -0.7 3,253,497 + PS417_14895 0.73 +0.3 3,253,500 + +5.0 3,253,501 - PS417_14895 0.73 +0.5 3,253,501 - PS417_14895 0.73 +1.7 3,253,501 - PS417_14895 0.73 +1.3 3,253,501 - PS417_14895 0.73 -1.3 3,253,542 + PS417_14895 0.77 -2.1 3,253,542 + PS417_14895 0.77 -1.0 3,253,696 - +3.5 3,253,696 - -0.4 3,253,809 + +0.3 3,253,946 - -0.1 3,253,946 - +0.5 3,253,946 - -0.1 3,253,949 + -2.0 3,253,950 - -0.5 3,253,991 - +0.9 3,253,991 - -0.7 3,254,458 + PS417_14900 0.40 +0.1 3,254,459 - PS417_14900 0.40 -0.6 3,254,533 + PS417_14900 0.46 +0.4 3,254,656 + PS417_14900 0.56 +3.4 3,254,656 + PS417_14900 0.56 -3.0 3,254,656 + PS417_14900 0.56 +0.3 3,254,656 + PS417_14900 0.56 -1.1 3,254,657 - PS417_14900 0.56 -2.2 3,254,750 - PS417_14900 0.63 -1.9 3,255,063 - PS417_14900 0.87 +0.3 3,255,063 - +1.8 3,255,064 + PS417_14900 0.87 -0.3 3,255,064 + PS417_14900 0.87 -0.7 3,255,064 + +3.1 3,255,195 + +1.8 3,255,483 + PS417_14905 0.41 +0.5 3,255,768 + PS417_14905 0.86 +0.1 3,255,880 + -0.2 3,255,880 + +0.8 3,256,096 + +3.3
Or see this region's nucleotide sequence