Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14875 and PS417_14880 are separated by 133 nucleotides PS417_14880 and PS417_14885 are separated by 43 nucleotides PS417_14885 and PS417_14890 overlap by 4 nucleotides
PS417_14875: PS417_14875 - polyisoprenoid-binding protein, at 3,249,543 to 3,250,097
_14875
PS417_14880: PS417_14880 - thioesterase, at 3,250,231 to 3,250,725
_14880
PS417_14885: PS417_14885 - dienelactone hydrolase, at 3,250,769 to 3,251,656
_14885
PS417_14890: PS417_14890 - LysR family transcriptional regulator, at 3,251,653 to 3,252,528
_14890
Position (kb)
3250
3251
3252 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3249.794 kb on - strand, within PS417_14875 at 3249.968 kb on - strand, within PS417_14875 at 3250.034 kb on - strand, within PS417_14875 at 3250.060 kb on + strand at 3250.061 kb on - strand at 3250.099 kb on - strand at 3250.099 kb on - strand at 3250.099 kb on - strand at 3250.193 kb on + strand at 3250.344 kb on - strand, within PS417_14880 at 3250.932 kb on - strand, within PS417_14885 at 3250.932 kb on - strand, within PS417_14885 at 3250.982 kb on + strand, within PS417_14885 at 3250.991 kb on + strand, within PS417_14885 at 3250.992 kb on - strand, within PS417_14885 at 3251.008 kb on + strand, within PS417_14885 at 3251.008 kb on + strand, within PS417_14885 at 3251.134 kb on + strand, within PS417_14885 at 3251.454 kb on - strand, within PS417_14885 at 3251.496 kb on - strand, within PS417_14885 at 3251.529 kb on - strand, within PS417_14885 at 3251.537 kb on + strand, within PS417_14885 at 3251.537 kb on + strand, within PS417_14885 at 3251.538 kb on - strand, within PS417_14885 at 3251.538 kb on - strand, within PS417_14885 at 3251.539 kb on + strand, within PS417_14885 at 3251.540 kb on - strand, within PS417_14885 at 3251.543 kb on + strand, within PS417_14885 at 3251.544 kb on - strand, within PS417_14885 at 3251.544 kb on - strand, within PS417_14885 at 3251.544 kb on - strand, within PS417_14885 at 3251.588 kb on + strand at 3251.641 kb on + strand at 3251.642 kb on - strand at 3251.645 kb on + strand at 3251.645 kb on + strand at 3251.738 kb on + strand at 3252.162 kb on + strand, within PS417_14890 at 3252.322 kb on + strand, within PS417_14890 at 3252.378 kb on + strand, within PS417_14890 at 3252.440 kb on - strand, within PS417_14890
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,249,794 - PS417_14875 0.45 +0.3 3,249,968 - PS417_14875 0.77 +0.0 3,250,034 - PS417_14875 0.88 +0.4 3,250,060 + +0.8 3,250,061 - +0.6 3,250,099 - -0.7 3,250,099 - -4.4 3,250,099 - -1.2 3,250,193 + +0.4 3,250,344 - PS417_14880 0.23 -0.0 3,250,932 - PS417_14885 0.18 -0.2 3,250,932 - PS417_14885 0.18 -0.9 3,250,982 + PS417_14885 0.24 +0.8 3,250,991 + PS417_14885 0.25 +0.6 3,250,992 - PS417_14885 0.25 +0.1 3,251,008 + PS417_14885 0.27 -1.7 3,251,008 + PS417_14885 0.27 -0.4 3,251,134 + PS417_14885 0.41 -2.7 3,251,454 - PS417_14885 0.77 -0.1 3,251,496 - PS417_14885 0.82 -2.2 3,251,529 - PS417_14885 0.86 +0.6 3,251,537 + PS417_14885 0.86 +0.9 3,251,537 + PS417_14885 0.86 -2.1 3,251,538 - PS417_14885 0.87 -1.1 3,251,538 - PS417_14885 0.87 -0.9 3,251,539 + PS417_14885 0.87 +0.0 3,251,540 - PS417_14885 0.87 -2.6 3,251,543 + PS417_14885 0.87 +0.1 3,251,544 - PS417_14885 0.87 +1.5 3,251,544 - PS417_14885 0.87 +0.6 3,251,544 - PS417_14885 0.87 +1.8 3,251,588 + +0.3 3,251,641 + +0.3 3,251,642 - -0.1 3,251,645 + -0.6 3,251,645 + -2.3 3,251,738 + -2.7 3,252,162 + PS417_14890 0.58 -0.6 3,252,322 + PS417_14890 0.76 -2.3 3,252,378 + PS417_14890 0.83 +2.7 3,252,440 - PS417_14890 0.90 +2.1
Or see this region's nucleotide sequence