Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14775 and PS417_14780 are separated by 143 nucleotides PS417_14780 and PS417_14785 are separated by 153 nucleotides PS417_14785 and PS417_14790 are separated by 51 nucleotides PS417_14790 and PS417_14800 are separated by 245 nucleotides
PS417_14775: PS417_14775 - major facilitator transporter, at 3,229,233 to 3,230,636
_14775
PS417_14780: PS417_14780 - GNAT family acetyltraansferase, at 3,230,780 to 3,231,310
_14780
PS417_14785: PS417_14785 - glycine/betaine ABC transporter, at 3,231,464 to 3,232,966
_14785
PS417_14790: PS417_14790 - diguanylate cyclase, at 3,233,018 to 3,233,521
_14790
PS417_14800: PS417_14800 - diguanylate cyclase, at 3,233,767 to 3,236,349
_14800
Position (kb)
3231
3232
3233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3230.590 kb on - strand at 3230.658 kb on + strand at 3230.658 kb on + strand at 3230.662 kb on + strand at 3230.669 kb on + strand at 3230.670 kb on - strand at 3230.706 kb on + strand at 3230.707 kb on - strand at 3231.037 kb on + strand, within PS417_14780 at 3231.084 kb on - strand, within PS417_14780 at 3231.084 kb on - strand, within PS417_14780 at 3231.084 kb on - strand, within PS417_14780 at 3231.084 kb on - strand, within PS417_14780 at 3231.161 kb on - strand, within PS417_14780 at 3231.378 kb on + strand at 3231.378 kb on + strand at 3231.451 kb on + strand at 3231.452 kb on - strand at 3231.472 kb on - strand at 3231.596 kb on + strand at 3231.611 kb on + strand at 3231.611 kb on + strand at 3231.612 kb on - strand at 3231.655 kb on - strand, within PS417_14785 at 3231.740 kb on - strand, within PS417_14785 at 3231.743 kb on + strand, within PS417_14785 at 3231.744 kb on - strand, within PS417_14785 at 3231.829 kb on + strand, within PS417_14785 at 3231.899 kb on + strand, within PS417_14785 at 3231.899 kb on + strand, within PS417_14785 at 3231.900 kb on - strand, within PS417_14785 at 3231.900 kb on - strand, within PS417_14785 at 3231.929 kb on + strand, within PS417_14785 at 3232.112 kb on + strand, within PS417_14785 at 3232.289 kb on + strand, within PS417_14785 at 3232.292 kb on + strand, within PS417_14785 at 3232.293 kb on - strand, within PS417_14785 at 3232.293 kb on - strand at 3232.293 kb on - strand, within PS417_14785 at 3232.293 kb on - strand, within PS417_14785 at 3232.307 kb on + strand, within PS417_14785 at 3232.307 kb on + strand, within PS417_14785 at 3232.308 kb on - strand, within PS417_14785 at 3232.308 kb on - strand, within PS417_14785 at 3232.308 kb on - strand, within PS417_14785 at 3232.319 kb on + strand, within PS417_14785 at 3232.320 kb on - strand, within PS417_14785 at 3232.320 kb on - strand, within PS417_14785 at 3232.341 kb on - strand, within PS417_14785 at 3232.625 kb on - strand, within PS417_14785 at 3232.718 kb on + strand, within PS417_14785 at 3232.718 kb on + strand, within PS417_14785 at 3232.718 kb on + strand, within PS417_14785 at 3232.718 kb on + strand, within PS417_14785 at 3232.718 kb on + strand, within PS417_14785 at 3232.719 kb on - strand, within PS417_14785 at 3232.730 kb on + strand, within PS417_14785 at 3232.942 kb on - strand at 3232.977 kb on + strand at 3232.977 kb on + strand at 3233.490 kb on + strand at 3233.500 kb on + strand at 3233.947 kb on + strand at 3233.947 kb on + strand at 3233.955 kb on + strand at 3233.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,230,590 - +0.5 3,230,658 + +0.2 3,230,658 + -0.1 3,230,662 + -0.5 3,230,669 + -0.7 3,230,670 - -0.4 3,230,706 + -0.5 3,230,707 - -0.3 3,231,037 + PS417_14780 0.48 -0.2 3,231,084 - PS417_14780 0.57 -2.5 3,231,084 - PS417_14780 0.57 +1.3 3,231,084 - PS417_14780 0.57 +0.7 3,231,084 - PS417_14780 0.57 +0.2 3,231,161 - PS417_14780 0.72 -3.3 3,231,378 + -0.7 3,231,378 + +0.7 3,231,451 + +0.4 3,231,452 - -0.2 3,231,472 - -0.1 3,231,596 + +0.1 3,231,611 + +1.3 3,231,611 + -0.7 3,231,612 - -3.0 3,231,655 - PS417_14785 0.13 -0.2 3,231,740 - PS417_14785 0.18 +0.8 3,231,743 + PS417_14785 0.19 +0.9 3,231,744 - PS417_14785 0.19 +1.3 3,231,829 + PS417_14785 0.24 -3.4 3,231,899 + PS417_14785 0.29 +1.6 3,231,899 + PS417_14785 0.29 -0.8 3,231,900 - PS417_14785 0.29 +0.1 3,231,900 - PS417_14785 0.29 +2.6 3,231,929 + PS417_14785 0.31 -2.6 3,232,112 + PS417_14785 0.43 +0.4 3,232,289 + PS417_14785 0.55 +1.3 3,232,292 + PS417_14785 0.55 -1.1 3,232,293 - PS417_14785 0.55 -0.1 3,232,293 - +3.2 3,232,293 - PS417_14785 0.55 -1.7 3,232,293 - PS417_14785 0.55 -0.1 3,232,307 + PS417_14785 0.56 -2.9 3,232,307 + PS417_14785 0.56 +2.7 3,232,308 - PS417_14785 0.56 -0.3 3,232,308 - PS417_14785 0.56 -0.3 3,232,308 - PS417_14785 0.56 +1.3 3,232,319 + PS417_14785 0.57 +0.0 3,232,320 - PS417_14785 0.57 -2.2 3,232,320 - PS417_14785 0.57 +1.6 3,232,341 - PS417_14785 0.58 -1.3 3,232,625 - PS417_14785 0.77 -2.0 3,232,718 + PS417_14785 0.83 +0.0 3,232,718 + PS417_14785 0.83 +0.7 3,232,718 + PS417_14785 0.83 -0.2 3,232,718 + PS417_14785 0.83 +1.5 3,232,718 + PS417_14785 0.83 -0.7 3,232,719 - PS417_14785 0.83 +1.4 3,232,730 + PS417_14785 0.84 +1.1 3,232,942 - +1.3 3,232,977 + -2.2 3,232,977 + +3.0 3,233,490 + -0.9 3,233,500 + -3.6 3,233,947 + -2.6 3,233,947 + -0.3 3,233,955 + -1.0 3,233,956 - +0.5
Or see this region's nucleotide sequence