Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14380 and PS417_14385 are separated by 348 nucleotides PS417_14385 and PS417_14390 are separated by 212 nucleotides PS417_14390 and PS417_14395 are separated by 236 nucleotides PS417_14395 and PS417_14400 are separated by 6 nucleotides PS417_14400 and PS417_14405 are separated by 111 nucleotides
PS417_14380: PS417_14380 - hypothetical protein, at 3,142,953 to 3,145,391
_14380
PS417_14385: PS417_14385 - tRNA-Cys, at 3,145,740 to 3,145,813
_14385
PS417_14390: PS417_14390 - acyl-homoserine lactone acylase subunit beta, at 3,146,026 to 3,148,311
_14390
PS417_14395: PS417_14395 - fe2+ zn2+ uptake regulation protein, at 3,148,548 to 3,148,928
_14395
PS417_14400: PS417_14400 - hypothetical protein, at 3,148,935 to 3,149,123
_14400
PS417_14405: PS417_14405 - ABC transporter permease, at 3,149,235 to 3,149,996
_14405
Position (kb)
3146
3147
3148
3149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3145.083 kb on + strand, within PS417_14380 at 3145.277 kb on - strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.470 kb on + strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.471 kb on - strand at 3145.472 kb on + strand at 3145.472 kb on + strand at 3145.493 kb on + strand at 3145.494 kb on - strand at 3145.567 kb on + strand at 3146.288 kb on + strand, within PS417_14390 at 3146.289 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3146.688 kb on - strand, within PS417_14390 at 3147.069 kb on - strand, within PS417_14390 at 3147.116 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.295 kb on - strand, within PS417_14390 at 3147.296 kb on + strand, within PS417_14390 at 3147.296 kb on + strand, within PS417_14390 at 3147.297 kb on - strand, within PS417_14390 at 3147.393 kb on + strand, within PS417_14390 at 3147.393 kb on + strand, within PS417_14390 at 3147.393 kb on + strand, within PS417_14390 at 3147.394 kb on - strand, within PS417_14390 at 3147.394 kb on - strand, within PS417_14390 at 3147.395 kb on + strand, within PS417_14390 at 3147.395 kb on + strand, within PS417_14390 at 3147.395 kb on + strand, within PS417_14390 at 3147.396 kb on - strand, within PS417_14390 at 3147.396 kb on - strand, within PS417_14390 at 3147.396 kb on - strand, within PS417_14390 at 3147.733 kb on + strand, within PS417_14390 at 3147.808 kb on + strand, within PS417_14390 at 3148.279 kb on - strand at 3148.353 kb on + strand at 3148.353 kb on + strand at 3148.353 kb on + strand at 3148.354 kb on - strand at 3148.364 kb on + strand at 3148.364 kb on + strand at 3148.364 kb on + strand at 3148.365 kb on - strand at 3148.407 kb on + strand at 3148.407 kb on + strand at 3148.493 kb on + strand at 3148.493 kb on + strand at 3148.493 kb on + strand at 3148.494 kb on - strand at 3148.551 kb on + strand at 3148.551 kb on + strand at 3148.551 kb on + strand at 3148.551 kb on + strand at 3148.552 kb on - strand at 3148.552 kb on - strand at 3148.552 kb on - strand at 3148.793 kb on - strand, within PS417_14395 at 3149.047 kb on + strand, within PS417_14400 at 3149.074 kb on - strand, within PS417_14400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,145,083 + PS417_14380 0.87 -2.0 3,145,277 - +0.9 3,145,470 + -2.4 3,145,470 + -3.1 3,145,470 + -0.0 3,145,470 + -2.7 3,145,470 + +0.9 3,145,470 + +0.3 3,145,471 - +0.6 3,145,471 - +1.2 3,145,471 - +2.9 3,145,471 - +0.1 3,145,471 - +1.3 3,145,472 + +0.7 3,145,472 + +1.7 3,145,493 + +0.4 3,145,494 - -3.1 3,145,567 + -0.5 3,146,288 + PS417_14390 0.11 +0.9 3,146,289 - PS417_14390 0.12 +0.5 3,146,688 - PS417_14390 0.29 +1.2 3,146,688 - PS417_14390 0.29 +0.8 3,146,688 - PS417_14390 0.29 +0.9 3,146,688 - PS417_14390 0.29 +0.0 3,147,069 - PS417_14390 0.46 -0.1 3,147,116 - PS417_14390 0.48 +0.6 3,147,295 - PS417_14390 0.56 +0.8 3,147,295 - PS417_14390 0.56 +0.5 3,147,295 - PS417_14390 0.56 -0.7 3,147,295 - PS417_14390 0.56 +0.1 3,147,295 - PS417_14390 0.56 +2.6 3,147,296 + PS417_14390 0.56 -1.3 3,147,296 + PS417_14390 0.56 +1.8 3,147,297 - PS417_14390 0.56 -2.4 3,147,393 + PS417_14390 0.60 -0.3 3,147,393 + PS417_14390 0.60 +0.4 3,147,393 + PS417_14390 0.60 +1.9 3,147,394 - PS417_14390 0.60 +0.0 3,147,394 - PS417_14390 0.60 +1.2 3,147,395 + PS417_14390 0.60 +1.7 3,147,395 + PS417_14390 0.60 +0.4 3,147,395 + PS417_14390 0.60 +1.1 3,147,396 - PS417_14390 0.60 +0.3 3,147,396 - PS417_14390 0.60 +0.8 3,147,396 - PS417_14390 0.60 +1.0 3,147,733 + PS417_14390 0.75 +1.6 3,147,808 + PS417_14390 0.78 -0.6 3,148,279 - -2.6 3,148,353 + +0.7 3,148,353 + -2.1 3,148,353 + -2.5 3,148,354 - -0.6 3,148,364 + -0.1 3,148,364 + +1.5 3,148,364 + +0.9 3,148,365 - -1.5 3,148,407 + -0.1 3,148,407 + +0.6 3,148,493 + +0.3 3,148,493 + -0.9 3,148,493 + -0.7 3,148,494 - -2.0 3,148,551 + -3.1 3,148,551 + +1.8 3,148,551 + +0.9 3,148,551 + -1.2 3,148,552 - -2.4 3,148,552 - -1.0 3,148,552 - -0.1 3,148,793 - PS417_14395 0.64 +1.0 3,149,047 + PS417_14400 0.59 +0.2 3,149,074 - PS417_14400 0.74 -0.2
Or see this region's nucleotide sequence