Strain Fitness in Pseudomonas simiae WCS417 around PS417_14220

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_14210 and PS417_14215 are separated by 125 nucleotidesPS417_14215 and PS417_14220 overlap by 29 nucleotidesPS417_14220 and PS417_14225 are separated by 196 nucleotidesPS417_14225 and PS417_14230 are separated by 180 nucleotidesPS417_14230 and PS417_14235 are separated by 248 nucleotides PS417_14210: PS417_14210 - hypothetical protein, at 3,114,211 to 3,114,900 _14210 PS417_14215: PS417_14215 - diguanylate cyclase, at 3,115,026 to 3,115,553 _14215 PS417_14220: PS417_14220 - aldehyde-activating protein, at 3,115,525 to 3,115,998 _14220 PS417_14225: PS417_14225 - hypothetical protein, at 3,116,195 to 3,116,476 _14225 PS417_14230: PS417_14230 - tRNA-Leu, at 3,116,657 to 3,116,743 _14230 PS417_14235: PS417_14235 - hypothetical protein, at 3,116,992 to 3,117,189 _14235 Position (kb) 3115 3116Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3114.883 kb on + strandat 3114.883 kb on + strandat 3114.989 kb on + strandat 3114.989 kb on + strandat 3114.990 kb on - strandat 3115.062 kb on + strandat 3115.063 kb on - strandat 3115.090 kb on - strandat 3115.298 kb on + strand, within PS417_14215at 3115.299 kb on - strand, within PS417_14215at 3115.300 kb on + strand, within PS417_14215at 3115.300 kb on + strand, within PS417_14215at 3115.300 kb on + strandat 3115.300 kb on + strandat 3115.301 kb on - strand, within PS417_14215at 3115.301 kb on - strand, within PS417_14215at 3115.301 kb on - strand, within PS417_14215at 3115.301 kb on - strand, within PS417_14215at 3115.301 kb on - strand, within PS417_14215at 3115.301 kb on - strandat 3115.301 kb on - strand, within PS417_14215at 3115.301 kb on - strand, within PS417_14215at 3115.404 kb on - strand, within PS417_14215at 3115.458 kb on - strand, within PS417_14215at 3115.526 kb on + strandat 3115.610 kb on + strand, within PS417_14220at 3115.610 kb on + strand, within PS417_14220at 3115.610 kb on + strand, within PS417_14220at 3115.711 kb on + strand, within PS417_14220at 3115.712 kb on - strand, within PS417_14220at 3115.779 kb on - strand, within PS417_14220at 3115.977 kb on - strandat 3115.977 kb on - strandat 3116.011 kb on - strandat 3116.014 kb on - strandat 3116.014 kb on - strandat 3116.103 kb on + strandat 3116.109 kb on + strandat 3116.110 kb on - strandat 3116.192 kb on + strandat 3116.192 kb on + strandat 3116.192 kb on + strandat 3116.192 kb on + strandat 3116.193 kb on - strandat 3116.193 kb on - strandat 3116.193 kb on - strandat 3116.193 kb on - strandat 3116.193 kb on - strandat 3116.193 kb on - strandat 3116.193 kb on - strandat 3116.193 kb on - strandat 3116.291 kb on - strand, within PS417_14225at 3116.475 kb on - strandat 3116.562 kb on + strandat 3116.563 kb on - strandat 3116.612 kb on + strandat 3116.775 kb on - strandat 3116.858 kb on + strandat 3116.859 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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3,114,883 + -0.0
3,114,883 + -0.1
3,114,989 + +2.2
3,114,989 + +0.6
3,114,990 - +1.6
3,115,062 + +0.8
3,115,063 - -0.5
3,115,090 - -1.2
3,115,298 + PS417_14215 0.52 -0.5
3,115,299 - PS417_14215 0.52 -4.2
3,115,300 + PS417_14215 0.52 -1.2
3,115,300 + PS417_14215 0.52 -1.8
3,115,300 + +0.3
3,115,300 + +2.3
3,115,301 - PS417_14215 0.52 -0.1
3,115,301 - PS417_14215 0.52 -1.5
3,115,301 - PS417_14215 0.52 -0.1
3,115,301 - PS417_14215 0.52 -0.3
3,115,301 - PS417_14215 0.52 -1.2
3,115,301 - -0.7
3,115,301 - PS417_14215 0.52 -0.1
3,115,301 - PS417_14215 0.52 -0.2
3,115,404 - PS417_14215 0.72 -1.2
3,115,458 - PS417_14215 0.82 -0.7
3,115,526 + -2.9
3,115,610 + PS417_14220 0.18 -3.2
3,115,610 + PS417_14220 0.18 +0.3
3,115,610 + PS417_14220 0.18 -0.3
3,115,711 + PS417_14220 0.39 +0.3
3,115,712 - PS417_14220 0.39 -1.7
3,115,779 - PS417_14220 0.54 -2.7
3,115,977 - -4.1
3,115,977 - +0.3
3,116,011 - +0.4
3,116,014 - -1.6
3,116,014 - +0.1
3,116,103 + -0.4
3,116,109 + -0.0
3,116,110 - +0.7
3,116,192 + -3.5
3,116,192 + +0.5
3,116,192 + +0.6
3,116,192 + -0.7
3,116,193 - +0.7
3,116,193 - +1.8
3,116,193 - -0.9
3,116,193 - -0.6
3,116,193 - -1.2
3,116,193 - +1.0
3,116,193 - -0.9
3,116,193 - +1.8
3,116,291 - PS417_14225 0.34 -1.9
3,116,475 - -1.1
3,116,562 + +1.3
3,116,563 - -1.0
3,116,612 + +0.8
3,116,775 - +0.2
3,116,858 + -2.1
3,116,859 - -1.0

Or see this region's nucleotide sequence