Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14210 and PS417_14215 are separated by 125 nucleotides PS417_14215 and PS417_14220 overlap by 29 nucleotides PS417_14220 and PS417_14225 are separated by 196 nucleotides PS417_14225 and PS417_14230 are separated by 180 nucleotides PS417_14230 and PS417_14235 are separated by 248 nucleotides
PS417_14210: PS417_14210 - hypothetical protein, at 3,114,211 to 3,114,900
_14210
PS417_14215: PS417_14215 - diguanylate cyclase, at 3,115,026 to 3,115,553
_14215
PS417_14220: PS417_14220 - aldehyde-activating protein, at 3,115,525 to 3,115,998
_14220
PS417_14225: PS417_14225 - hypothetical protein, at 3,116,195 to 3,116,476
_14225
PS417_14230: PS417_14230 - tRNA-Leu, at 3,116,657 to 3,116,743
_14230
PS417_14235: PS417_14235 - hypothetical protein, at 3,116,992 to 3,117,189
_14235
Position (kb)
3115
3116 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3114.883 kb on + strand at 3114.883 kb on + strand at 3114.989 kb on + strand at 3114.989 kb on + strand at 3114.990 kb on - strand at 3115.062 kb on + strand at 3115.063 kb on - strand at 3115.090 kb on - strand at 3115.298 kb on + strand, within PS417_14215 at 3115.299 kb on - strand, within PS417_14215 at 3115.300 kb on + strand, within PS417_14215 at 3115.300 kb on + strand, within PS417_14215 at 3115.300 kb on + strand at 3115.300 kb on + strand at 3115.301 kb on - strand, within PS417_14215 at 3115.301 kb on - strand, within PS417_14215 at 3115.301 kb on - strand, within PS417_14215 at 3115.301 kb on - strand, within PS417_14215 at 3115.301 kb on - strand, within PS417_14215 at 3115.301 kb on - strand at 3115.301 kb on - strand, within PS417_14215 at 3115.301 kb on - strand, within PS417_14215 at 3115.404 kb on - strand, within PS417_14215 at 3115.458 kb on - strand, within PS417_14215 at 3115.526 kb on + strand at 3115.610 kb on + strand, within PS417_14220 at 3115.610 kb on + strand, within PS417_14220 at 3115.610 kb on + strand, within PS417_14220 at 3115.711 kb on + strand, within PS417_14220 at 3115.712 kb on - strand, within PS417_14220 at 3115.779 kb on - strand, within PS417_14220 at 3115.977 kb on - strand at 3115.977 kb on - strand at 3116.011 kb on - strand at 3116.014 kb on - strand at 3116.014 kb on - strand at 3116.103 kb on + strand at 3116.109 kb on + strand at 3116.110 kb on - strand at 3116.192 kb on + strand at 3116.192 kb on + strand at 3116.192 kb on + strand at 3116.192 kb on + strand at 3116.193 kb on - strand at 3116.193 kb on - strand at 3116.193 kb on - strand at 3116.193 kb on - strand at 3116.193 kb on - strand at 3116.193 kb on - strand at 3116.193 kb on - strand at 3116.193 kb on - strand at 3116.291 kb on - strand, within PS417_14225 at 3116.475 kb on - strand at 3116.562 kb on + strand at 3116.563 kb on - strand at 3116.612 kb on + strand at 3116.775 kb on - strand at 3116.858 kb on + strand at 3116.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,114,883 + -0.0 3,114,883 + -0.1 3,114,989 + +2.2 3,114,989 + +0.6 3,114,990 - +1.6 3,115,062 + +0.8 3,115,063 - -0.5 3,115,090 - -1.2 3,115,298 + PS417_14215 0.52 -0.5 3,115,299 - PS417_14215 0.52 -4.2 3,115,300 + PS417_14215 0.52 -1.2 3,115,300 + PS417_14215 0.52 -1.8 3,115,300 + +0.3 3,115,300 + +2.3 3,115,301 - PS417_14215 0.52 -0.1 3,115,301 - PS417_14215 0.52 -1.5 3,115,301 - PS417_14215 0.52 -0.1 3,115,301 - PS417_14215 0.52 -0.3 3,115,301 - PS417_14215 0.52 -1.2 3,115,301 - -0.7 3,115,301 - PS417_14215 0.52 -0.1 3,115,301 - PS417_14215 0.52 -0.2 3,115,404 - PS417_14215 0.72 -1.2 3,115,458 - PS417_14215 0.82 -0.7 3,115,526 + -2.9 3,115,610 + PS417_14220 0.18 -3.2 3,115,610 + PS417_14220 0.18 +0.3 3,115,610 + PS417_14220 0.18 -0.3 3,115,711 + PS417_14220 0.39 +0.3 3,115,712 - PS417_14220 0.39 -1.7 3,115,779 - PS417_14220 0.54 -2.7 3,115,977 - -4.1 3,115,977 - +0.3 3,116,011 - +0.4 3,116,014 - -1.6 3,116,014 - +0.1 3,116,103 + -0.4 3,116,109 + -0.0 3,116,110 - +0.7 3,116,192 + -3.5 3,116,192 + +0.5 3,116,192 + +0.6 3,116,192 + -0.7 3,116,193 - +0.7 3,116,193 - +1.8 3,116,193 - -0.9 3,116,193 - -0.6 3,116,193 - -1.2 3,116,193 - +1.0 3,116,193 - -0.9 3,116,193 - +1.8 3,116,291 - PS417_14225 0.34 -1.9 3,116,475 - -1.1 3,116,562 + +1.3 3,116,563 - -1.0 3,116,612 + +0.8 3,116,775 - +0.2 3,116,858 + -2.1 3,116,859 - -1.0
Or see this region's nucleotide sequence