Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13980 and PS417_13985 are separated by 35 nucleotides PS417_13985 and PS417_13990 are separated by 100 nucleotides PS417_13990 and PS417_13995 are separated by 79 nucleotides
PS417_13980: PS417_13980 - MFS transporter permease, at 3,071,384 to 3,072,694
_13980
PS417_13985: PS417_13985 - dehydrogenase, at 3,072,730 to 3,073,434
_13985
PS417_13990: PS417_13990 - LysR family transcriptional regulator, at 3,073,535 to 3,074,437
_13990
PS417_13995: PS417_13995 - NADP-dependent oxidoreductase, at 3,074,517 to 3,075,527
_13995
Position (kb)
3073
3074
3075 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3072.572 kb on + strand at 3072.573 kb on - strand at 3072.573 kb on - strand at 3072.574 kb on + strand at 3072.692 kb on + strand at 3072.693 kb on - strand at 3072.800 kb on + strand at 3072.801 kb on - strand, within PS417_13985 at 3072.913 kb on - strand, within PS417_13985 at 3072.913 kb on - strand, within PS417_13985 at 3072.913 kb on - strand, within PS417_13985 at 3073.129 kb on + strand, within PS417_13985 at 3073.130 kb on - strand, within PS417_13985 at 3074.049 kb on - strand, within PS417_13990 at 3074.049 kb on - strand, within PS417_13990 at 3074.098 kb on + strand, within PS417_13990 at 3074.099 kb on - strand, within PS417_13990 at 3074.166 kb on + strand, within PS417_13990 at 3074.404 kb on - strand at 3074.490 kb on + strand at 3074.491 kb on - strand at 3074.491 kb on - strand at 3074.491 kb on - strand at 3074.513 kb on - strand at 3074.513 kb on - strand at 3074.865 kb on + strand at 3074.865 kb on + strand, within PS417_13995 at 3074.865 kb on + strand, within PS417_13995 at 3074.865 kb on + strand, within PS417_13995 at 3074.865 kb on + strand, within PS417_13995 at 3074.865 kb on + strand, within PS417_13995 at 3074.866 kb on - strand, within PS417_13995 at 3074.866 kb on - strand, within PS417_13995 at 3074.869 kb on - strand, within PS417_13995 at 3075.051 kb on + strand, within PS417_13995 at 3075.052 kb on - strand, within PS417_13995 at 3075.052 kb on - strand, within PS417_13995 at 3075.126 kb on + strand at 3075.126 kb on + strand, within PS417_13995 at 3075.128 kb on + strand, within PS417_13995 at 3075.162 kb on + strand, within PS417_13995 at 3075.276 kb on + strand, within PS417_13995 at 3075.391 kb on - strand, within PS417_13995 at 3075.393 kb on - strand, within PS417_13995 at 3075.393 kb on - strand, within PS417_13995
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,072,572 + +0.9 3,072,573 - +0.1 3,072,573 - -1.3 3,072,574 + -1.4 3,072,692 + +0.5 3,072,693 - +0.1 3,072,800 + -2.4 3,072,801 - PS417_13985 0.10 +1.2 3,072,913 - PS417_13985 0.26 +0.4 3,072,913 - PS417_13985 0.26 -0.9 3,072,913 - PS417_13985 0.26 -1.5 3,073,129 + PS417_13985 0.57 -2.2 3,073,130 - PS417_13985 0.57 -1.3 3,074,049 - PS417_13990 0.57 +1.5 3,074,049 - PS417_13990 0.57 +0.3 3,074,098 + PS417_13990 0.62 +1.3 3,074,099 - PS417_13990 0.62 +1.6 3,074,166 + PS417_13990 0.70 +2.7 3,074,404 - +1.6 3,074,490 + +0.3 3,074,491 - +0.3 3,074,491 - -1.1 3,074,491 - +1.2 3,074,513 - -2.9 3,074,513 - -0.5 3,074,865 + +0.9 3,074,865 + PS417_13995 0.34 -0.3 3,074,865 + PS417_13995 0.34 -0.8 3,074,865 + PS417_13995 0.34 +1.7 3,074,865 + PS417_13995 0.34 -0.3 3,074,865 + PS417_13995 0.34 +0.9 3,074,866 - PS417_13995 0.35 -0.0 3,074,866 - PS417_13995 0.35 +1.0 3,074,869 - PS417_13995 0.35 -0.9 3,075,051 + PS417_13995 0.53 -0.9 3,075,052 - PS417_13995 0.53 +0.7 3,075,052 - PS417_13995 0.53 -0.0 3,075,126 + -0.7 3,075,126 + PS417_13995 0.60 -0.2 3,075,128 + PS417_13995 0.60 -0.2 3,075,162 + PS417_13995 0.64 +2.1 3,075,276 + PS417_13995 0.75 -2.1 3,075,391 - PS417_13995 0.86 -0.0 3,075,393 - PS417_13995 0.87 -0.6 3,075,393 - PS417_13995 0.87 -0.9
Or see this region's nucleotide sequence