Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13720 and PS417_13725 are separated by 8 nucleotides PS417_13725 and PS417_13730 are separated by 86 nucleotides PS417_13730 and PS417_13735 overlap by 1 nucleotides PS417_13735 and PS417_13740 are separated by 101 nucleotides PS417_13740 and PS417_13745 are separated by 101 nucleotides
PS417_13720: PS417_13720 - hypothetical protein, at 3,017,219 to 3,017,734
_13720
PS417_13725: PS417_13725 - membrane protein, at 3,017,743 to 3,018,186
_13725
PS417_13730: PS417_13730 - hypothetical protein, at 3,018,273 to 3,018,512
_13730
PS417_13735: PS417_13735 - hypothetical protein, at 3,018,512 to 3,018,766
_13735
PS417_13740: PS417_13740 - hypothetical protein, at 3,018,868 to 3,019,149
_13740
PS417_13745: PS417_13745 - hypothetical protein, at 3,019,251 to 3,020,009
_13745
Position (kb)
3018
3019 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3017.337 kb on + strand, within PS417_13720 at 3017.396 kb on + strand, within PS417_13720 at 3017.397 kb on - strand, within PS417_13720 at 3017.397 kb on - strand, within PS417_13720 at 3017.397 kb on - strand, within PS417_13720 at 3017.682 kb on + strand, within PS417_13720 at 3017.726 kb on + strand at 3017.756 kb on + strand at 3017.774 kb on - strand at 3017.801 kb on + strand, within PS417_13725 at 3017.945 kb on + strand, within PS417_13725 at 3017.945 kb on + strand, within PS417_13725 at 3017.946 kb on - strand, within PS417_13725 at 3018.082 kb on - strand, within PS417_13725 at 3018.111 kb on + strand, within PS417_13725 at 3018.111 kb on + strand, within PS417_13725 at 3018.250 kb on - strand at 3018.275 kb on - strand at 3018.275 kb on - strand at 3018.275 kb on - strand at 3018.388 kb on + strand, within PS417_13730 at 3018.407 kb on - strand, within PS417_13730 at 3018.488 kb on + strand, within PS417_13730 at 3018.489 kb on - strand at 3018.658 kb on + strand, within PS417_13735 at 3018.659 kb on - strand, within PS417_13735 at 3018.743 kb on - strand at 3018.796 kb on + strand at 3018.797 kb on - strand at 3018.882 kb on + strand at 3019.097 kb on - strand, within PS417_13740 at 3019.097 kb on - strand, within PS417_13740 at 3019.097 kb on - strand, within PS417_13740 at 3019.117 kb on + strand, within PS417_13740 at 3019.118 kb on - strand at 3019.118 kb on - strand, within PS417_13740 at 3019.118 kb on - strand, within PS417_13740 at 3019.118 kb on - strand, within PS417_13740 at 3019.164 kb on + strand at 3019.166 kb on + strand at 3019.166 kb on + strand at 3019.166 kb on + strand at 3019.344 kb on + strand, within PS417_13745 at 3019.344 kb on + strand, within PS417_13745 at 3019.344 kb on + strand, within PS417_13745 at 3019.344 kb on + strand, within PS417_13745 at 3019.345 kb on - strand, within PS417_13745 at 3019.345 kb on - strand, within PS417_13745 at 3019.451 kb on + strand, within PS417_13745 at 3019.451 kb on + strand, within PS417_13745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,017,337 + PS417_13720 0.23 -0.4 3,017,396 + PS417_13720 0.34 -0.9 3,017,397 - PS417_13720 0.34 -0.5 3,017,397 - PS417_13720 0.34 +0.4 3,017,397 - PS417_13720 0.34 -0.5 3,017,682 + PS417_13720 0.90 +0.5 3,017,726 + -1.3 3,017,756 + -0.9 3,017,774 - -4.0 3,017,801 + PS417_13725 0.13 +0.9 3,017,945 + PS417_13725 0.45 +1.4 3,017,945 + PS417_13725 0.45 +0.7 3,017,946 - PS417_13725 0.46 +0.1 3,018,082 - PS417_13725 0.76 -0.9 3,018,111 + PS417_13725 0.83 -0.4 3,018,111 + PS417_13725 0.83 -0.5 3,018,250 - -0.4 3,018,275 - +1.2 3,018,275 - -0.7 3,018,275 - -0.7 3,018,388 + PS417_13730 0.48 -2.8 3,018,407 - PS417_13730 0.56 +0.1 3,018,488 + PS417_13730 0.90 -2.1 3,018,489 - -0.1 3,018,658 + PS417_13735 0.57 -0.9 3,018,659 - PS417_13735 0.58 -0.0 3,018,743 - +0.9 3,018,796 + -0.9 3,018,797 - -3.1 3,018,882 + +0.6 3,019,097 - PS417_13740 0.81 -0.3 3,019,097 - PS417_13740 0.81 -1.8 3,019,097 - PS417_13740 0.81 -1.7 3,019,117 + PS417_13740 0.88 +0.3 3,019,118 - +0.9 3,019,118 - PS417_13740 0.89 +0.3 3,019,118 - PS417_13740 0.89 -1.8 3,019,118 - PS417_13740 0.89 +0.3 3,019,164 + +0.1 3,019,166 + -1.6 3,019,166 + -0.1 3,019,166 + -0.8 3,019,344 + PS417_13745 0.12 -1.3 3,019,344 + PS417_13745 0.12 -0.8 3,019,344 + PS417_13745 0.12 -2.0 3,019,344 + PS417_13745 0.12 +4.6 3,019,345 - PS417_13745 0.12 +1.1 3,019,345 - PS417_13745 0.12 +1.5 3,019,451 + PS417_13745 0.26 +1.6 3,019,451 + PS417_13745 0.26 +0.7
Or see this region's nucleotide sequence