Strain Fitness in Pseudomonas simiae WCS417 around PS417_13730

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_13720 and PS417_13725 are separated by 8 nucleotidesPS417_13725 and PS417_13730 are separated by 86 nucleotidesPS417_13730 and PS417_13735 overlap by 1 nucleotidesPS417_13735 and PS417_13740 are separated by 101 nucleotidesPS417_13740 and PS417_13745 are separated by 101 nucleotides PS417_13720: PS417_13720 - hypothetical protein, at 3,017,219 to 3,017,734 _13720 PS417_13725: PS417_13725 - membrane protein, at 3,017,743 to 3,018,186 _13725 PS417_13730: PS417_13730 - hypothetical protein, at 3,018,273 to 3,018,512 _13730 PS417_13735: PS417_13735 - hypothetical protein, at 3,018,512 to 3,018,766 _13735 PS417_13740: PS417_13740 - hypothetical protein, at 3,018,868 to 3,019,149 _13740 PS417_13745: PS417_13745 - hypothetical protein, at 3,019,251 to 3,020,009 _13745 Position (kb) 3018 3019Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 3017.337 kb on + strand, within PS417_13720at 3017.396 kb on + strand, within PS417_13720at 3017.397 kb on - strand, within PS417_13720at 3017.397 kb on - strand, within PS417_13720at 3017.397 kb on - strand, within PS417_13720at 3017.682 kb on + strand, within PS417_13720at 3017.726 kb on + strandat 3017.756 kb on + strandat 3017.774 kb on - strandat 3017.801 kb on + strand, within PS417_13725at 3017.945 kb on + strand, within PS417_13725at 3017.945 kb on + strand, within PS417_13725at 3017.946 kb on - strand, within PS417_13725at 3018.082 kb on - strand, within PS417_13725at 3018.111 kb on + strand, within PS417_13725at 3018.111 kb on + strand, within PS417_13725at 3018.250 kb on - strandat 3018.275 kb on - strandat 3018.275 kb on - strandat 3018.275 kb on - strandat 3018.388 kb on + strand, within PS417_13730at 3018.407 kb on - strand, within PS417_13730at 3018.488 kb on + strand, within PS417_13730at 3018.489 kb on - strandat 3018.658 kb on + strand, within PS417_13735at 3018.659 kb on - strand, within PS417_13735at 3018.743 kb on - strandat 3018.796 kb on + strandat 3018.797 kb on - strandat 3018.882 kb on + strandat 3019.097 kb on - strand, within PS417_13740at 3019.097 kb on - strand, within PS417_13740at 3019.097 kb on - strand, within PS417_13740at 3019.117 kb on + strand, within PS417_13740at 3019.118 kb on - strandat 3019.118 kb on - strand, within PS417_13740at 3019.118 kb on - strand, within PS417_13740at 3019.118 kb on - strand, within PS417_13740at 3019.164 kb on + strandat 3019.166 kb on + strandat 3019.166 kb on + strandat 3019.166 kb on + strandat 3019.344 kb on + strand, within PS417_13745at 3019.344 kb on + strand, within PS417_13745at 3019.344 kb on + strand, within PS417_13745at 3019.344 kb on + strand, within PS417_13745at 3019.345 kb on - strand, within PS417_13745at 3019.345 kb on - strand, within PS417_13745at 3019.451 kb on + strand, within PS417_13745at 3019.451 kb on + strand, within PS417_13745

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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3,017,337 + PS417_13720 0.23 -0.4
3,017,396 + PS417_13720 0.34 -0.9
3,017,397 - PS417_13720 0.34 -0.5
3,017,397 - PS417_13720 0.34 +0.4
3,017,397 - PS417_13720 0.34 -0.5
3,017,682 + PS417_13720 0.90 +0.5
3,017,726 + -1.3
3,017,756 + -0.9
3,017,774 - -4.0
3,017,801 + PS417_13725 0.13 +0.9
3,017,945 + PS417_13725 0.45 +1.4
3,017,945 + PS417_13725 0.45 +0.7
3,017,946 - PS417_13725 0.46 +0.1
3,018,082 - PS417_13725 0.76 -0.9
3,018,111 + PS417_13725 0.83 -0.4
3,018,111 + PS417_13725 0.83 -0.5
3,018,250 - -0.4
3,018,275 - +1.2
3,018,275 - -0.7
3,018,275 - -0.7
3,018,388 + PS417_13730 0.48 -2.8
3,018,407 - PS417_13730 0.56 +0.1
3,018,488 + PS417_13730 0.90 -2.1
3,018,489 - -0.1
3,018,658 + PS417_13735 0.57 -0.9
3,018,659 - PS417_13735 0.58 -0.0
3,018,743 - +0.9
3,018,796 + -0.9
3,018,797 - -3.1
3,018,882 + +0.6
3,019,097 - PS417_13740 0.81 -0.3
3,019,097 - PS417_13740 0.81 -1.8
3,019,097 - PS417_13740 0.81 -1.7
3,019,117 + PS417_13740 0.88 +0.3
3,019,118 - +0.9
3,019,118 - PS417_13740 0.89 +0.3
3,019,118 - PS417_13740 0.89 -1.8
3,019,118 - PS417_13740 0.89 +0.3
3,019,164 + +0.1
3,019,166 + -1.6
3,019,166 + -0.1
3,019,166 + -0.8
3,019,344 + PS417_13745 0.12 -1.3
3,019,344 + PS417_13745 0.12 -0.8
3,019,344 + PS417_13745 0.12 -2.0
3,019,344 + PS417_13745 0.12 +4.6
3,019,345 - PS417_13745 0.12 +1.1
3,019,345 - PS417_13745 0.12 +1.5
3,019,451 + PS417_13745 0.26 +1.6
3,019,451 + PS417_13745 0.26 +0.7

Or see this region's nucleotide sequence