Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13620 and PS417_13625 are separated by 181 nucleotides PS417_13625 and PS417_13635 are separated by 164 nucleotides PS417_13635 and PS417_13640 overlap by 8 nucleotides
PS417_13620: PS417_13620 - thermostable hemolysin, at 2,998,739 to 2,999,413
_13620
PS417_13625: PS417_13625 - cytochrome B561, at 2,999,595 to 3,000,137
_13625
PS417_13635: PS417_13635 - D-ribose transporter ATP-binding protein, at 3,000,302 to 3,001,834
_13635
PS417_13640: PS417_13640 - ribose ABC transporter permease, at 3,001,827 to 3,002,804
_13640
Position (kb)
3000
3001
3002 Strain fitness (log2 ratio)
-1
0
1 at 2999.357 kb on - strand at 2999.622 kb on + strand at 2999.622 kb on + strand at 2999.623 kb on - strand at 2999.679 kb on + strand, within PS417_13625 at 2999.680 kb on - strand, within PS417_13625 at 2999.867 kb on + strand, within PS417_13625 at 2999.868 kb on - strand, within PS417_13625 at 2999.883 kb on + strand, within PS417_13625 at 2999.884 kb on - strand, within PS417_13625 at 3000.217 kb on + strand at 3000.218 kb on - strand at 3000.894 kb on - strand, within PS417_13635 at 3000.894 kb on - strand, within PS417_13635 at 3000.963 kb on - strand, within PS417_13635 at 3001.043 kb on + strand, within PS417_13635 at 3001.043 kb on + strand, within PS417_13635 at 3001.065 kb on - strand, within PS417_13635 at 3001.067 kb on - strand, within PS417_13635 at 3001.771 kb on + strand at 3002.418 kb on + strand, within PS417_13640 at 3002.545 kb on + strand, within PS417_13640 at 3002.730 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,999,357 - +1.5 2,999,622 + +1.1 2,999,622 + +0.9 2,999,623 - +1.6 2,999,679 + PS417_13625 0.15 +1.2 2,999,680 - PS417_13625 0.16 +0.3 2,999,867 + PS417_13625 0.50 -0.1 2,999,868 - PS417_13625 0.50 +1.2 2,999,883 + PS417_13625 0.53 +0.7 2,999,884 - PS417_13625 0.53 +0.0 3,000,217 + +1.4 3,000,218 - +0.4 3,000,894 - PS417_13635 0.39 +0.7 3,000,894 - PS417_13635 0.39 -0.8 3,000,963 - PS417_13635 0.43 +0.3 3,001,043 + PS417_13635 0.48 +0.0 3,001,043 + PS417_13635 0.48 +1.4 3,001,065 - PS417_13635 0.50 +1.2 3,001,067 - PS417_13635 0.50 +0.9 3,001,771 + +0.7 3,002,418 + PS417_13640 0.60 -0.3 3,002,545 + PS417_13640 0.73 +0.3 3,002,730 + +1.6
Or see this region's nucleotide sequence