Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13485 and PS417_13490 are separated by 40 nucleotides PS417_13490 and PS417_13495 are separated by 186 nucleotides PS417_13495 and PS417_13500 overlap by 4 nucleotides
PS417_13485: PS417_13485 - lipase, at 2,971,548 to 2,972,960
_13485
PS417_13490: PS417_13490 - hypothetical protein, at 2,973,001 to 2,973,225
_13490
PS417_13495: PS417_13495 - nuclease, at 2,973,412 to 2,975,058
_13495
PS417_13500: PS417_13500 - ATP-dependent DNA helicase, at 2,975,055 to 2,977,310
_13500
Position (kb)
2973
2974
2975
2976 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2972.603 kb on + strand, within PS417_13485 at 2972.651 kb on + strand, within PS417_13485 at 2972.651 kb on + strand, within PS417_13485 at 2972.831 kb on - strand at 2972.843 kb on - strand at 2972.994 kb on + strand at 2973.122 kb on + strand, within PS417_13490 at 2973.123 kb on - strand, within PS417_13490 at 2973.337 kb on + strand at 2973.337 kb on + strand at 2973.338 kb on - strand at 2973.342 kb on - strand at 2973.342 kb on - strand at 2973.342 kb on - strand at 2973.439 kb on + strand at 2973.439 kb on + strand at 2973.443 kb on - strand at 2973.443 kb on - strand at 2973.619 kb on + strand, within PS417_13495 at 2973.619 kb on + strand, within PS417_13495 at 2973.620 kb on - strand, within PS417_13495 at 2973.693 kb on - strand, within PS417_13495 at 2973.869 kb on - strand, within PS417_13495 at 2973.970 kb on + strand, within PS417_13495 at 2973.970 kb on + strand, within PS417_13495 at 2973.970 kb on + strand, within PS417_13495 at 2973.971 kb on - strand, within PS417_13495 at 2974.122 kb on + strand, within PS417_13495 at 2974.358 kb on + strand, within PS417_13495 at 2974.738 kb on + strand, within PS417_13495 at 2975.061 kb on + strand at 2975.062 kb on - strand at 2975.197 kb on - strand at 2975.216 kb on - strand at 2975.216 kb on - strand at 2975.302 kb on - strand at 2975.344 kb on + strand, within PS417_13500 at 2975.344 kb on + strand, within PS417_13500 at 2975.345 kb on - strand, within PS417_13500 at 2975.345 kb on - strand, within PS417_13500 at 2975.368 kb on - strand, within PS417_13500 at 2975.424 kb on + strand, within PS417_13500 at 2975.637 kb on + strand at 2975.706 kb on - strand, within PS417_13500 at 2975.934 kb on + strand, within PS417_13500 at 2975.935 kb on - strand, within PS417_13500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,972,603 + PS417_13485 0.75 -2.1 2,972,651 + PS417_13485 0.78 +0.2 2,972,651 + PS417_13485 0.78 -0.1 2,972,831 - +1.1 2,972,843 - -1.7 2,972,994 + +1.1 2,973,122 + PS417_13490 0.54 -0.5 2,973,123 - PS417_13490 0.54 -0.4 2,973,337 + -1.9 2,973,337 + +0.5 2,973,338 - -0.1 2,973,342 - -2.4 2,973,342 - -1.8 2,973,342 - +0.3 2,973,439 + -0.2 2,973,439 + +0.4 2,973,443 - -0.5 2,973,443 - -0.4 2,973,619 + PS417_13495 0.13 -1.3 2,973,619 + PS417_13495 0.13 -1.6 2,973,620 - PS417_13495 0.13 +1.9 2,973,693 - PS417_13495 0.17 +1.8 2,973,869 - PS417_13495 0.28 -3.1 2,973,970 + PS417_13495 0.34 +1.3 2,973,970 + PS417_13495 0.34 +0.2 2,973,970 + PS417_13495 0.34 -0.7 2,973,971 - PS417_13495 0.34 +3.2 2,974,122 + PS417_13495 0.43 -0.5 2,974,358 + PS417_13495 0.57 -1.1 2,974,738 + PS417_13495 0.81 -0.0 2,975,061 + -0.4 2,975,062 - +0.1 2,975,197 - -1.2 2,975,216 - +1.2 2,975,216 - -0.4 2,975,302 - -0.4 2,975,344 + PS417_13500 0.13 +1.2 2,975,344 + PS417_13500 0.13 -1.3 2,975,345 - PS417_13500 0.13 +1.0 2,975,345 - PS417_13500 0.13 +0.9 2,975,368 - PS417_13500 0.14 -2.8 2,975,424 + PS417_13500 0.16 +0.8 2,975,637 + +1.7 2,975,706 - PS417_13500 0.29 +0.9 2,975,934 + PS417_13500 0.39 -2.0 2,975,935 - PS417_13500 0.39 -0.9
Or see this region's nucleotide sequence