Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01320 and PS417_01325 overlap by 4 nucleotides PS417_01325 and PS417_01330 overlap by 4 nucleotides PS417_01330 and PS417_01335 are separated by 151 nucleotides
PS417_01320: PS417_01320 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 286,327 to 286,872
_01320
PS417_01325: PS417_01325 - glucose-1-phosphate thymidylyltransferase, at 286,869 to 287,744
_01325
PS417_01330: PS417_01330 - dTDP-glucose 4,6-dehydratase, at 287,741 to 288,823
_01330
PS417_01335: PS417_01335 - hypothetical protein, at 288,975 to 291,875
_01335
Position (kb) 287
288
289 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 287.790 kb on + strand at 288.868 kb on + strand at 288.868 kb on + strand at 288.869 kb on - strand at 288.945 kb on + strand at 288.946 kb on - strand at 288.946 kb on - strand at 288.946 kb on - strand at 289.009 kb on + strand at 289.063 kb on - strand at 289.114 kb on - strand at 289.114 kb on - strand at 289.114 kb on - strand at 289.209 kb on - strand at 289.314 kb on + strand, within PS417_01335 at 289.315 kb on - strand, within PS417_01335 at 289.315 kb on - strand, within PS417_01335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 287,790 + -0.5 288,868 + +0.0 288,868 + +0.6 288,869 - +0.2 288,945 + -1.8 288,946 - -3.0 288,946 - +0.4 288,946 - -0.2 289,009 + -1.5 289,063 - -1.2 289,114 - -2.5 289,114 - -0.2 289,114 - -2.5 289,209 - +0.2 289,314 + PS417_01335 0.12 -1.1 289,315 - PS417_01335 0.12 -0.0 289,315 - PS417_01335 0.12 -0.9
Or see this region's nucleotide sequence