Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01305 and PS417_01310 overlap by 4 nucleotides PS417_01310 and PS417_01315 are separated by 36 nucleotides PS417_01315 and PS417_01320 overlap by 4 nucleotides
PS417_01305: PS417_01305 - Fis family transcriptional regulator, at 282,243 to 283,619
_01305
PS417_01310: PS417_01310 - ATPase, at 283,616 to 285,424
_01310
PS417_01315: PS417_01315 - dTDP-4-dehydrorhamnose reductase, at 285,461 to 286,330
_01315
PS417_01320: PS417_01320 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 286,327 to 286,872
_01320
Position (kb) 283
284
285
286 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 282.701 kb on - strand, within PS417_01305 at 282.703 kb on + strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 283.315 kb on + strand, within PS417_01305 at 283.619 kb on + strand at 283.619 kb on + strand at 283.619 kb on + strand at 283.620 kb on - strand at 283.620 kb on - strand at 283.620 kb on - strand at 283.800 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 284.941 kb on - strand, within PS417_01310 at 285.102 kb on + strand, within PS417_01310 at 285.456 kb on + strand at 285.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 282,701 - PS417_01305 0.33 +0.1 282,703 + PS417_01305 0.33 -2.0 282,704 - PS417_01305 0.33 +0.0 282,704 - PS417_01305 0.33 -0.4 282,704 - PS417_01305 0.33 -1.0 283,315 + PS417_01305 0.78 +1.7 283,619 + +1.3 283,619 + -0.9 283,619 + -0.0 283,620 - +0.3 283,620 - +0.7 283,620 - +1.6 283,800 + PS417_01310 0.10 -1.5 283,863 + PS417_01310 0.14 +0.2 283,863 + PS417_01310 0.14 -0.0 283,863 + PS417_01310 0.14 +0.6 284,941 - PS417_01310 0.73 +0.2 285,102 + PS417_01310 0.82 +3.2 285,456 + +0.5 285,457 - -0.8
Or see this region's nucleotide sequence