Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12875 and PS417_12880 overlap by 33 nucleotides PS417_12880 and PS417_12885 are separated by 176 nucleotides PS417_12885 and PS417_12890 are separated by 149 nucleotides
PS417_12875: PS417_12875 - NADH pyrophosphatase, at 2,816,004 to 2,816,837
_12875
PS417_12880: PS417_12880 - membrane protein, at 2,816,805 to 2,817,584
_12880
PS417_12885: PS417_12885 - phosphatidylcholine synthase, at 2,817,761 to 2,818,480
_12885
PS417_12890: PS417_12890 - methyltransferase, at 2,818,630 to 2,819,544
_12890
Position (kb)
2817
2818
2819 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2816.791 kb on - strand at 2817.227 kb on - strand, within PS417_12880 at 2817.564 kb on + strand at 2817.595 kb on + strand at 2817.595 kb on + strand at 2817.596 kb on - strand at 2817.690 kb on + strand at 2817.691 kb on - strand at 2817.806 kb on - strand at 2817.822 kb on + strand at 2817.823 kb on - strand at 2817.925 kb on + strand, within PS417_12885 at 2818.042 kb on + strand, within PS417_12885 at 2818.042 kb on + strand, within PS417_12885 at 2818.044 kb on + strand at 2818.044 kb on + strand, within PS417_12885 at 2818.045 kb on - strand, within PS417_12885 at 2818.045 kb on - strand, within PS417_12885 at 2818.194 kb on + strand, within PS417_12885 at 2818.223 kb on + strand, within PS417_12885 at 2818.223 kb on + strand, within PS417_12885 at 2818.223 kb on + strand, within PS417_12885 at 2818.224 kb on - strand, within PS417_12885 at 2818.224 kb on - strand, within PS417_12885 at 2818.224 kb on - strand, within PS417_12885 at 2818.227 kb on + strand, within PS417_12885 at 2818.227 kb on + strand, within PS417_12885 at 2818.228 kb on - strand, within PS417_12885 at 2818.228 kb on - strand, within PS417_12885 at 2818.237 kb on - strand at 2818.410 kb on - strand at 2818.427 kb on - strand at 2818.460 kb on + strand at 2818.461 kb on - strand at 2818.475 kb on + strand at 2818.606 kb on + strand at 2818.795 kb on + strand, within PS417_12890 at 2818.795 kb on + strand, within PS417_12890 at 2818.796 kb on - strand, within PS417_12890 at 2818.812 kb on + strand, within PS417_12890 at 2819.050 kb on + strand, within PS417_12890 at 2819.050 kb on + strand at 2819.051 kb on - strand, within PS417_12890 at 2819.051 kb on - strand, within PS417_12890 at 2819.097 kb on + strand, within PS417_12890 at 2819.315 kb on + strand, within PS417_12890 at 2819.316 kb on - strand, within PS417_12890
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,816,791 - +0.7 2,817,227 - PS417_12880 0.54 +1.9 2,817,564 + +1.4 2,817,595 + -0.3 2,817,595 + +0.8 2,817,596 - +3.0 2,817,690 + -3.0 2,817,691 - +0.2 2,817,806 - +0.1 2,817,822 + -0.1 2,817,823 - +0.6 2,817,925 + PS417_12885 0.23 +1.7 2,818,042 + PS417_12885 0.39 +2.0 2,818,042 + PS417_12885 0.39 -0.7 2,818,044 + +0.8 2,818,044 + PS417_12885 0.39 +0.5 2,818,045 - PS417_12885 0.39 -3.0 2,818,045 - PS417_12885 0.39 -0.6 2,818,194 + PS417_12885 0.60 +0.8 2,818,223 + PS417_12885 0.64 +1.0 2,818,223 + PS417_12885 0.64 +1.1 2,818,223 + PS417_12885 0.64 -3.6 2,818,224 - PS417_12885 0.64 -1.3 2,818,224 - PS417_12885 0.64 +0.5 2,818,224 - PS417_12885 0.64 +2.4 2,818,227 + PS417_12885 0.65 +0.7 2,818,227 + PS417_12885 0.65 -1.5 2,818,228 - PS417_12885 0.65 +1.8 2,818,228 - PS417_12885 0.65 +0.7 2,818,237 - -0.7 2,818,410 - +0.6 2,818,427 - +1.0 2,818,460 + -0.0 2,818,461 - +1.6 2,818,475 + +0.7 2,818,606 + -0.8 2,818,795 + PS417_12890 0.18 +0.4 2,818,795 + PS417_12890 0.18 +1.5 2,818,796 - PS417_12890 0.18 +0.8 2,818,812 + PS417_12890 0.20 -1.2 2,819,050 + PS417_12890 0.46 -0.7 2,819,050 + -1.0 2,819,051 - PS417_12890 0.46 -0.5 2,819,051 - PS417_12890 0.46 +1.3 2,819,097 + PS417_12890 0.51 -1.0 2,819,315 + PS417_12890 0.75 -1.8 2,819,316 - PS417_12890 0.75 -1.1
Or see this region's nucleotide sequence